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An acid, 2,2-dichloropropionic acid (2,2-DCP) is an active ingredient in herbicide (Dalapon®). Using 2,2-DCP as a model substrate, an alkalotolerant bacterium was successfully isolated from the Blue Lake, Turkey. This bacterium is a potential bioremediation agent of recalcitrant xenobiotic halogenated compounds. This study aimed to prove the efficacy of the alkalotolerance Bacillus megaterium BHS1 in degrading 2,2-DCP as the sole source of carbon. Biolog GEN III system and 16S rRNA analysis were used for the identification of the bacterium. It was discovered that the strain BHS1 is Bacillus m ...Более
The aim of this study was to isolate and identify Antarctica microalgae collected through the National Antarctic Science Expedition and to examine the growth responses and biochemical contents of Chlorella variabilis under cold and temperate cultivation conditions in growth medium consisting of various nitrogen concentrations under different lighting cycle parameters. In this study, the Antarctic microalgae Chlorella variabilis YTU.ANTARCTIC.001, was cultivated at different temperatures of 4, 13, 20 and 27 °C in modified BG-11 medium with various concentrations of NaNO3 (0, 0.75, 1.5, 2.5 and ...Более
Bermet KIDIRALİYEVA | Nurcamal ÖMÜRZAKOVA | Yılmaz KAYA
Çeltik, Antarktika hariç tüm kıtalarda yetiştirilen dünyanın en değerli gıda ürünleri arasında yer almaktadır. Küresel ölçekte, ekim alanı açısından değerlendirildiğinde buğdaydan sonra ikinci sırada yer almakla birlikte verimi yaklaşık olarak toplam buğday verimine eşittir. Kırgızistan, çeltik yetiştiriciliği bakımından önde gelen ülkeler arasında yer almamakta, bu bölgede sadece yerel ölçekte üretim yapılmaktadır. Kırgızistan’ın güney bölgesi çeltik tarımına elverişli olduğu için son 10 yılda nispeten çeltik ekim alanları ve tüketimi artmıştır. Çeltiğe ilginin artmasına; nüfus artışı, ekonom ...Более
This study presents the initial structural model of L-haloacid dehalogenase (DehLBHS1) from Bacillus megaterium BHS1, an alkalotolerant bacterium known for its ability to degrade halogenated environmental pollutants. The model provides insights into the structural features of DehLBHS1 and expands our understanding of the enzymatic mechanisms involved in the degradation of these hazardous pollutants. Key amino acid residues (Arg40, Phe59, Asn118, Asn176, and Trp178) in DehLBHS1 were identified to play critical roles in catalysis and molecular recognition of haloalkanoic acid, essential for effi ...Более
Changing environmental conditions can cause diseases in plants, leading to novel interactions between plants and stress factors. In this respect, the relationships between pathogens and plants regarding epigenetics have still been investigated. Our aim is to examine the effect of leaf blight bacterial causal agent, Pantoea ananatis isolate PaTo34a1, on barley- and rice-specific retrotransposons’ (Nikita, Osr30 and RIRE1, respectively) movements in different rice cultivars (Oryza sativa L. cv. Tosya, Cameo, Arize, Ronaldo, Osmancik-97, Kale, Baldo, Bafra, Hakkari and Vasco) collected from diffe ...Более
Tomato is one of the best-studied cultivated dicotyledonous plants in molecular studies. Mobile genetic elements constitute large parts of plant genomes. Retrotransposons are mobile genetic elements within the genome and constitute more than 60 of the tomato genome.Transposable elements (TE) or transposons are DNA sequences that can alter their position within a genome, cause mutations and change the genetic identity of the cells and genome size. We aimed to analyze potato specific-P-Tst-1, P-Tst-3 and P-Tst-6 retrotransposon movements in tomatoes at different developmental stages (mature seed ...Более
Mumio is a valuable substance that ranges in color from pale brown to blackish-brown and is obtained from rock layers in various mountain ranges worldwide, particularly in the Tien Shan ranges of Kyrgyzstan. It is also a natural chemical found largely in the Himalayas, created over ages by the progressive degradation of particular plants by bacteria. Mumio is essentially a nutraceutical product with considerable human health advantages. To fully appreciate the predicted impact of mumio use in the medical area, particularly in neurological sciences, more research at both the fundamental biology ...Более
There are mounting concerns over the high concentrations of non-biogenic, toxic halogenated organic compounds being liberated into the ecosystem. Therefore, this study’s isolation of a novel bacterium from a contaminated stream in Fatsa, Ordu, Turkey, adept in degrading 2,2-dichloropropionic (of 2,2-DCP) is a welcome endeavor. The ability of the bacterial isolate to utilize 2,2-DCP as the sole carbon and energy source was discovered when the bacterium was observed to grow well on liquid minimal media containing 20 mM of 2,2-DCP, showing a doubling time of 14.2 h. The following genetic and bioc ...Более
Agrobacterium-mediated transformation of upland rice is established in few numbers of cultivars due to the high cultivar-specificity of regeneration from transformed explants. Further, dehalogenase E (dehE) gene had been characterized in Pseudomonas putida and it produces an enzyme that degrades dalapon. This study aimed to transform Turkish upland rice with the dehE herbicide resistant gene and addresses the challenges of transgenic rice recovery by identifying explant and transformation method. Constructed vector pCAMdehE carrying dehE gene was transferred into the rice shoot apex by Agrobac ...Более
Halophiles are extremophilic microorganisms that grow optimally at high salt concentrations by producing a myriad of equally halotolerant enzymes. Structural haloadaptation of these enzymes adept to thriving under high-salt environments, though are not fully understood. Herein, the study attempts an in silico investigation to identify and comprehend the evolutionary structural adaptation of a halotolerant dehalogenase, DehHX (GenBank accession number: KR297065) of the halotolerant Pseudomonas halophila, over its non-halotolerant counterpart, DehMX1 (GenBank accession number KY129692) produced ...Более