There are mounting concerns over the high concentrations of non-biogenic, toxic halogenated organic compounds being liberated into the ecosystem. Therefore, this study’s isolation of a novel bacterium from a contaminated stream in Fatsa, Ordu, Turkey, adept in degrading 2,2-dichloropropionic (of 2,2-DCP) is a welcome endeavor. The ability of the bacterial isolate to utilize 2,2-DCP as the sole carbon and energy source was discovered when the bacterium was observed to grow well on liquid minimal media containing 20 mM of 2,2-DCP, showing a doubling time of 14.2 h. The following genetic and biochemical characterizations revealed that the 16S rRNA sequence of the fatsa001strain is identical (99) to Pseudomonas fluorescence, after which the sequence was deposited in the NCBI GenBank as Pseudomonas sp. strain fatsa001 (MN098848). The halogen-degrading ability of the P. fluorescens fatsa001 bacterium was again confirmed by the PCR data, which showed the presence of a conserved group of amino acids from the group I dehalogenase gene. It worth mentioning here that this is the first report on a P. fluorescence bacterial strain with the ability to degrade toxic 2,2-DCP. The detoxification ability of this bacterium envisages its practicality as an in situ environmental bioremediation agent.
Keyword: dalapon herbicide; dehalogenase; dehalogenation; recalcitrant
Название публикации (dc.title) | A 2,2-dichloropropionic acid-degrading novel Pseudomonas fluorescence strain fatsa001: isolation, identification, and characterization |
Автор/ы (dc.contributor.yazarlar) | Ulku Binboga Meral, Mohamed Faraj Edbeib, Suleyman Faruk Kirkinci, Hasan Murat Aksoy, Ayhan Akman, Roswanira Abdul Wahab, Fahrul Huyop, Gulbubu Kurmanbekova, Yunus Emre Arvas, Yilmaz Kaya |
Вид публикации (dc.type) | Makale |
Язык (dc.language) | İngilizce |
Год публикации (dc.date.issued) | 2024 |
Национальный/Международный (dc.identifier.ulusaluluslararasi) | Uluslararası |
Источник (dc.relation.journal) | Bioremediation Journal |
Номер (dc.identifier.issue) | Published online: 27 Feb 2024 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 1088-9868; Online ISSN: 1547-6529 |
Издатель (dc.publisher) | Taylor & Francis |
Базы данных (dc.contributor.veritaban) | Web of Science Core Collection |
Базы данных (dc.contributor.veritaban) | Taylor & Francis |
Базы данных (dc.contributor.veritaban) | Scopus |
Вид индекса (dc.identifier.index) | SCI Expanded |
Вид индекса (dc.identifier.index) | Scopus |
Импакт-фактор (dc.identifier.etkifaktoru) | 2 / 2022-WOS / Son 5 yıl: 2,3 |
Резюме (dc.description.abstract) | There are mounting concerns over the high concentrations of non-biogenic, toxic halogenated organic compounds being liberated into the ecosystem. Therefore, this study’s isolation of a novel bacterium from a contaminated stream in Fatsa, Ordu, Turkey, adept in degrading 2,2-dichloropropionic (of 2,2-DCP) is a welcome endeavor. The ability of the bacterial isolate to utilize 2,2-DCP as the sole carbon and energy source was discovered when the bacterium was observed to grow well on liquid minimal media containing 20 mM of 2,2-DCP, showing a doubling time of 14.2 h. The following genetic and biochemical characterizations revealed that the 16S rRNA sequence of the fatsa001strain is identical (99) to Pseudomonas fluorescence, after which the sequence was deposited in the NCBI GenBank as Pseudomonas sp. strain fatsa001 (MN098848). The halogen-degrading ability of the P. fluorescens fatsa001 bacterium was again confirmed by the PCR data, which showed the presence of a conserved group of amino acids from the group I dehalogenase gene. It worth mentioning here that this is the first report on a P. fluorescence bacterial strain with the ability to degrade toxic 2,2-DCP. The detoxification ability of this bacterium envisages its practicality as an in situ environmental bioremediation agent. |
Резюме (dc.description.abstract) | Keyword: dalapon herbicide; dehalogenase; dehalogenation; recalcitrant |
URL (dc.rights) | https://www.tandfonline.com/doi/full/10.1080/10889868.2024.2322466 |
DOI (dc.identifier.doi) | 10.1080/10889868.2024.2322466 |
Факультет / Институт (dc.identifier.fakulte) | Fen Fakültesi |
Кафедра (dc.identifier.bolum) | Biyoloji Bölümü |
Автор(ы) в учреждении (dc.contributor.author) | Gülbübü KURMANBEKOVA |
Автор(ы) в учреждении (dc.contributor.author) | Yılmaz KAYA |
№ регистрации (dc.identifier.kayitno) | BL7AA23A52 |
Дата регистрации (dc.date.available) | 2024-03-13 |
Заметка (Год публикации) (dc.identifier.notyayinyili) | WOS, Scopus Early Access: 27 Feb 2024 |
Wos No (dc.identifier.wos) | WOS:001175990700001 |
Тематический рубрикатор (dc.subject) | dalapon herbicide |
Тематический рубрикатор (dc.subject) | dehalogenase |
Тематический рубрикатор (dc.subject) | dehalogenation |
Тематический рубрикатор (dc.subject) | recalcitrant |