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Найденный: 28 Экземпляр 0.003 sn
Ученые : Saykal BOBUŞOVA ✕
Коллекция [2]
Ученые [7]
Устойчивое развитие ООН [6]
Факультет / Институт [2]
Вид публикации [3]
Год публикации [13]
Язык [2]
Источник [22]
Базы данных [16]
Вид индекса [6]
Вид индекса 2 [1]
Издатель [14]
Кафедра [2]
Национальный/Международный [2]
Тематический рубрикатор [35]
Dr. Saykal BOBUŞOVAZiraat Fakültesi
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Characterization of Pseudomonas syringae PV. syringae from diseased stone fruits in Kyrgyzstan and testing of biological agents against pathogen

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan. The conventional phenotypic (LOPAT, API tests) and PCR-assisted isolation were used for the identificationof Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach (Prunus persica), cherry (Prunus subgen), apricot (Prunus armeniaca), and plum (Prunus salicina) samples taken from the Chy, Is ...Более

Статья 2020 Ziraat Fakültesi International Journal of Phytopathology9 ( 2 ) 16 Просмотры
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An app for apples: Citizen-led mapping of fire blight in Central Asia

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Fire blight, caused by the bacterial pathogen Erwinia amylovora, is a severe bacterial disease of apple and pear that can quickly destroy whole plants. In the last decade, it was also detected in Central Asia, where wild pomaceous fruit plants represent the dominant species in mid-altitude forests and constitute a critical foundation for the entire ecosystem. Efficiently informing farmers, forestry services and private persons about the instances and dangers of fire blight, the correct way to recognize the symptoms, and the methods of disease control is thus of paramount importance in a vast a ...Более

Статья 2023 Ziraat Fakültesi Journal of Plant Pathology ( Published online: 30 May 2023 ) 36 Просмотры
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Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS se ...Более

Статья 2023 Ziraat Fakültesi Journal of Fungi9 ( 10 ) 26 Просмотры
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Identification of Microbial Populations Present in Agricultural and Nonarable Soils in the Talas Valley, Northern Kyrgyzstan, in Autumn

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Soil bacterial and fungal communities were investigated in relation to soil type and farm management practices after vegetation harvesting in autumn. Soils from fields cultivated with Phaseolus vulgaris (bean) and Pyrus comminus (pear) and nonarable, natural areas were studied. Microbial diversity was analysed using cultivation-dependent methods (isolation of pure cultures) and cultivation-independent methods (direct extraction of DNA from soil, followed by PCR amplification of the 16S rRNA and 18S rRNA genes). The use of cultivation-dependent methods revealed that there were no differences in ...Более

Статья 2023 Ziraat Fakültesi Applied and Environmental Soil Science2023 17 Просмотры
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In vitro and in vivo screening of bacterial species from contaminated soil for heavy metal biotransformation activity

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

Heavy metals (HMs) are widely used in various industries. High concentrations of HMs can be severely toxic to plants, animals and humans. Microorganism-based bioremediation has shown significant potential in degrading and detoxifying specific HM contaminants. In this study, we cultivated a range of bacterial strains in liquid and solid nutrient medium containing different concentrations of different HMs to select and analyze bacteria capable of transforming HMs. The bacterial strains most resistant to selected HMs and exhibiting the ability to remove HMs from contaminated soils were identified ...Более

Статья 2024 Ziraat Fakültesi Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes ( Published online: 27 Apr 2024 ) 0 Просмотры
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In situ microbial bioremediation of obsolete pesticides at their disposal sites

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

Soil pollution by pesticides is a global problem in many countries, including Kyrgyzstan. Currently, 50 storage facilities for obsolete banned pesticides exist, severely threatening the surrounding populations and environment. In this study, in situ bioremediation technology was used to remove pesticide contamination from soil around the storage facilities. Three conditions were tested: application of fertile soil and bioproduct, application of fertile soil and no bioproduct (Control 1), and application of fertile soil and no bioproduct (Control 2). An aerobic bioremediation approach was used: ...Более

Статья 2024 Ziraat Fakültesi Bioremediation Journal ( Published online: 25 Apr 2024 ) 0 Просмотры
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Микробные сообщества высокогорных и низкотемпературных почв долины Сон-Куль Кыргызстана

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

С помощью классического метода микробиологии и анализа последовательностей 16SrRNA впервые было изучено количественное соотношение и биоразнообразие микробных комплексов низкотемпературных почв долины Сон-Куль. Структура видового состава бактерий отличалась в почвах под различным покровам растительности по мере возрастания высоты местности. Анализ последовательностей 16SrRNA показал, что в летний период в почвах высокогорной долины Сон-Куль доминирующими филогенетическими группами были Actinobacteria (55,0%), Gammaproteobacteria ( 33,0%) и Firmicutes phyla (22,0%). Видовой состав обнаруженных ...Более

Статья 2012 Ziraat Fakültesi Фундаментальные исследования ( 9; Часть 2 ) 79 Просмотры
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Screening of Wild-Type Fungal Isolates for Cellulolytic Activity

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Background: In this study, wild-type fungal isolates, producing highly effective cellulolytic enzymes were selected for bioconversion of residues and waste from agriculture and rational utilization of energy resources for food production. Methods: We screened wild-type fungal isolates of Aspergillus, Penicillium, and Trichoderma with an enhanced ability to produce extracellular cellulase. We carried out solid-state fermentation on a medium of agricultural waste products, including wheat bran, beet peels, and cotton oil cake, as well as additional sources of nitrogen and mineral elements. Enzym ...Более

Статья 2011 Ziraat Fakültesi Microbiology Insights ( 4 ) 74 Просмотры
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Biological Activity of Ag and Cu Monometallic Nanoparticles and Ag-Cu Bimetallic Nanocomposites against Plant Pathogens and Seeds

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

The antibacterial activities of silver (Ag) and copper (Cu) monometallic and bimetallic nanoparticles (MNPs and BNPs) against the most economically important and common plant bacterial diseases in Kyrgyzstan were tested, as well as their phytotoxic effects on wheat and corn seeds. To achieve this, a range of Ag and Cu MNPs and BNPs were synthesized using different methods and were tested at different concentrations to compare their biological effects. The antibacterial activities of Ag and Cu MNPs and Ag-Cu BNPs against the plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and ...Более

Статья 2022 Ziraat Fakültesi Journal of Nanomaterials2022 95 Просмотры
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Microbial communities of vegetable seeds and biocontrol microbes for seed treatment

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Thirty-one species of vegetable seeds from Kyrgyzstan's State Register were analysed for mycological and bacterial composition using various methods, from simple visual to molecular. The modified roll-towel method simultaneously assesses the degree of contamination and seed germination. Screening in agar medium allows analysis of species on the outer and inner surfaces of vegetable seeds that are not detected using the roll-towel method. As a result of the phytopathological examination, 15 fungal and three bacterial species were found. Direct DNA extraction and amplification of the universal 1 ...Более

Статья 2022 Ziraat Fakültesi Seed Science and Technology50 ( 1-3 ) 34 Просмотры
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LOW TEMPERATURE AND VEGETATION EFFECTS ON THE SOIL BACTERIAL COMMUNITIES STRUCTURE IN HIGH MOUNTAINOUS AND COLD BIOTOPES IN KYRGYZSTAN

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

It is well known that soil microorganisms play essential roles in the biogeochemical cycling of biogenic elements and soil-forming processes. However, little is known about the effect of the vegetation type on the bacterial community structures in soils from cold regions. For these reasons, we have analysed the bacterial communities of eight biotopes covered with different plants and two biotopes without vegetation in the Son-Kull Valley as the coldest corner in Kyrgyzstan. Using the culture-depended and culture-independent (16S rRNA gene sequencing) methods, we found 4 phylum (Actinobacteria- ...Более

Статья 2022 Ziraat Fakültesi Applied Ecology and Environmental Research20 ( 5 ) 43 Просмотры
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Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bac ...Более

Статья 2019 Ziraat Fakültesi European Journal of Plant Pathology155 ( 2 ) 55 Просмотры
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