Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bacteria among isolates. The assignment to this taxonomic species has been corroborated by phylogenetic reconstruction using multilocus sequence analysis, and a short-sequence repeat (SSR) marker has been employed to estimate genetic diversity across the isolates. CRISPR analysis has revealed both a previously unreported CRISPR-2 array pattern and a close relationship of Kyrgyz E. amylovora isolates to strains present in Europe and the Middle East. This study presents the first consistent molecular taxonomic characterization of E. amylovora bacteria from Kyrgyzstan.
Название публикации (dc.title) | Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan |
Автор/ы (dc.contributor.yazarlar) | Tinatin Doolotkeldieva, Saykal Bobusheva, Christina Schuster, Maxabat Konurbaeva, Andreas Leclerque |
Вид публикации (dc.type) | Makale |
Язык (dc.language) | İngilizce |
Год публикации (dc.date.issued) | 2019 |
Национальный/Международный (dc.identifier.ulusaluluslararasi) | Uluslararası |
Источник (dc.relation.journal) | European Journal of Plant Pathology |
Номер (dc.identifier.issue) | 2 |
Том/№ (dc.identifier.volume) | 155 |
Страница (dc.identifier.startpage) | 677-686 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 0929-1873; Online ISSN: 1573-8469 |
Издатель (dc.publisher) | Springer |
Базы данных (dc.contributor.veritaban) | Web of Science Core Collection |
Базы данных (dc.contributor.veritaban) | Springer |
Базы данных (dc.contributor.veritaban) | Scopus |
Вид индекса (dc.identifier.index) | SCI Expanded |
Вид индекса (dc.identifier.index) | Scopus |
Импакт-фактор (dc.identifier.etkifaktoru) | 1,744 / 2018-WOS / 5 Year: 1,754 |
Резюме (dc.description.abstract) | Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bacteria among isolates. The assignment to this taxonomic species has been corroborated by phylogenetic reconstruction using multilocus sequence analysis, and a short-sequence repeat (SSR) marker has been employed to estimate genetic diversity across the isolates. CRISPR analysis has revealed both a previously unreported CRISPR-2 array pattern and a close relationship of Kyrgyz E. amylovora isolates to strains present in Europe and the Middle East. This study presents the first consistent molecular taxonomic characterization of E. amylovora bacteria from Kyrgyzstan. |
URL (dc.rights) | https://link.springer.com/article/10.1007/s10658-019-01790-3 |
DOI (dc.identifier.doi) | 10.1007/s10658-019-01790-3 |
Факультет / Институт (dc.identifier.fakulte) | Ziraat Fakültesi |
Кафедра (dc.identifier.bolum) | Bitki Koruma Bölümü |
Автор(ы) в учреждении (dc.contributor.author) | Tinatin DOOLOTKELDİEVA |
Автор(ы) в учреждении (dc.contributor.author) | Saykal BOBUŞOVA |
Автор(ы) в учреждении (dc.contributor.author) | Mahabat KONURBAYEVA |
№ регистрации (dc.identifier.kayitno) | BLB5C86959 |
Дата регистрации (dc.date.available) | 2019-10-31 |
Заметка (Год публикации) (dc.identifier.notyayinyili) | October 2019 |
Wos No (dc.identifier.wos) | WOS:000490304400025 |
Тематический рубрикатор (dc.subject) | fire blight |
Тематический рубрикатор (dc.subject) | erwinia amylovora |
Тематический рубрикатор (dc.subject) | Kyrgyzstan |
Тематический рубрикатор (dc.subject) | diagnostic PCR |
Тематический рубрикатор (dc.subject) | multilocus sequence analysis (MLSA) |
Тематический рубрикатор (dc.subject) | short-sequence repeat (SSR) |
Тематический рубрикатор (dc.subject) | CRISPR arrays |