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After maize, wheat and rice, barley is the most widely planted and economically important cereal crop in the Kyrgyzstan's highlands (Usubaliev et al., 2013) and worldwide. In two barley fields in the Manas district of Bishkek, Kyrgyzstan, diseased plants exhibiting crown and root rot, stunting and brown discolouration on internodes were observed in 2020. Disease incidence was approximately 5 in the two fields.Small sections of root and crown tissues from diseased plants were surface sterilised in 1 (v/v) sodium hypochlorite solution for one minute before being placed onto potato dextrose agar ...Более
İsmail ERPER | Şenol YILDIZ | Sezim COLDOŞBEKOVA | Tair Esenalı Uulu
Fungal species associated with crown and root rot diseases in wheat have been extensively studied in many parts of the world. However, no reports on the relative importance and distribution of pathogens associated with wheat crown and root rot in Kyrgyzstan have been published. Hence, fungal species associated with wheat crown/root rot were surveyed in three main wheat production regions in northern Kyrgyzstan. Fungal species were isolated on 1/5 strength potato-dextrose agar amended with streptomycin (0.1 g/L) and chloramphenicol (0.05 g/L). A total of 598 fungal isolates from symptomatic tis ...Более
The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan. The conventional phenotypic (LOPAT, API tests) and PCR-assisted isolation were used for the identificationof Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach (Prunus persica), cherry (Prunus subgen), apricot (Prunus armeniaca), and plum (Prunus salicina) samples taken from the Chy, Is ...Более
In this study, morphological and molecular characterizations of twenty-four Heterodera populations (cereal cyst nematodes, CCNs) collected from wheat production fields in Turkey were carried out. Light microscopy, species-specific markers, RFLP, and ITS sequencing were used to identify the nematode populations. The obtained CCN populations were identified as Heterodera avenae, H. filipjevi, and H. latipons according to the morphometric analysis, which was confirmed by the molecular techniques. The ITS region sequencing analysis confirmed the species identification, and phylogenetic analysis of ...Более
Erwinia amylovora as a fire blight pathogen and Venturia inaequalis as a scab pathogen were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical and pathogenicity tests, alongside PCR analyses were conducted to identify the local isolates of Erwinia amylovora and Venturia inaequalis. The alternative antagonistic microorganisms which combat bacterium E. amylovora and fungus Venturia inaequalis were tested within in vitro and in vivo conditions. The results revealed ...Более
The study investigated the status of cereal cyst nematodes (CCN) in the main wheat-growing areas of Kyrgyzstan in 2020. Soil samples were taken from 69 different wheat fields located in Chuy and Issyk-Kul provinces. CCN were found in thirty-one out of the sixty-nine locations surveyed. The highest occurrence of CCN was in the Tyup location in Issyk-Kul province with 81 cysts (250 cm3 soil)-1. The CCN populations were identified by both morphological and molecular analyses. According to the results, all populations were identified as Heterodera filipjevi. No variations in rDNA-ITS sequencing da ...Более
Fire blight, caused by the bacterial pathogen Erwinia amylovora, is a severe bacterial disease of apple and pear that can quickly destroy whole plants. In the last decade, it was also detected in Central Asia, where wild pomaceous fruit plants represent the dominant species in mid-altitude forests and constitute a critical foundation for the entire ecosystem. Efficiently informing farmers, forestry services and private persons about the instances and dangers of fire blight, the correct way to recognize the symptoms, and the methods of disease control is thus of paramount importance in a vast a ...Более
center dot Citrus plants are host to several plant parasitic nematodes (PPNs), which are microscopic organisms. Among PPNs, the citrus root nematode, T. semipenetrans (Cobb 1913) (Tylenchida: Tylenchulidae), causes significant damage to citrus plantations worldwide. Understanding citrus nematode populations, precise identification, host preference among citrus species, and damage threshold are crucial to control T. semipenetrans. The minutiae of citrus plant-nematode interactions, nematode density and molecular nematode identification are not well understood. In this study, nematode species an ...Более
Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS se ...Более
Soil bacterial and fungal communities were investigated in relation to soil type and farm management practices after vegetation harvesting in autumn. Soils from fields cultivated with Phaseolus vulgaris (bean) and Pyrus comminus (pear) and nonarable, natural areas were studied. Microbial diversity was analysed using cultivation-dependent methods (isolation of pure cultures) and cultivation-independent methods (direct extraction of DNA from soil, followed by PCR amplification of the 16S rRNA and 18S rRNA genes). The use of cultivation-dependent methods revealed that there were no differences in ...Более
Heavy metals (HMs) are widely used in various industries. High concentrations of HMs can be severely toxic to plants, animals and humans. Microorganism-based bioremediation has shown significant potential in degrading and detoxifying specific HM contaminants. In this study, we cultivated a range of bacterial strains in liquid and solid nutrient medium containing different concentrations of different HMs to select and analyze bacteria capable of transforming HMs. The bacterial strains most resistant to selected HMs and exhibiting the ability to remove HMs from contaminated soils were identified ...Более