Prof. Dr. Tinatin DOOLOTKELDİEVAZiraat Fakültesi

Characterization of Pseudomonas syringae PV. syringae from diseased stone fruits in Kyrgyzstan and testing of biological agents against pathogen

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan. The conventional phenotypic (LOPAT, API tests) and PCR-assisted isolation were used for the identificationof Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach (Prunus persica), cherry (Prunus subgen), apricot (Prunus armeniaca), and plum (Prunus salicina) samples taken from the Chy, Is ...Daha fazlası

Makale 2020 Ziraat Fakültesi International Journal of Phytopathology9 ( 2 ) 16 Görüntülenme

Streptomyces bio-products against apple and pear diseases in organic orchards

Tinatin DOOLOTKELDİEVA

Erwinia amylovora as a fire blight pathogen and Venturia inaequalis as a scab pathogen were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical and pathogenicity tests, alongside PCR analyses were conducted to identify the local isolates of Erwinia amylovora and Venturia inaequalis. The alternative antagonistic microorganisms which combat bacterium E. amylovora and fungus Venturia inaequalis were tested within in vitro and in vivo conditions. The results revealed ...Daha fazlası

Kitap Bölümü 2018 Ziraat Fakültesi Agricultural Research Updates22 ( Chapter 2 ) 16 Görüntülenme

An app for apples: Citizen-led mapping of fire blight in Central Asia

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Fire blight, caused by the bacterial pathogen Erwinia amylovora, is a severe bacterial disease of apple and pear that can quickly destroy whole plants. In the last decade, it was also detected in Central Asia, where wild pomaceous fruit plants represent the dominant species in mid-altitude forests and constitute a critical foundation for the entire ecosystem. Efficiently informing farmers, forestry services and private persons about the instances and dangers of fire blight, the correct way to recognize the symptoms, and the methods of disease control is thus of paramount importance in a vast a ...Daha fazlası

Makale 2023 Ziraat Fakültesi Journal of Plant Pathology ( Published online: 30 May 2023 ) 36 Görüntülenme

Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS se ...Daha fazlası

Makale 2023 Ziraat Fakültesi Journal of Fungi9 ( 10 ) 26 Görüntülenme

Identification of Microbial Populations Present in Agricultural and Nonarable Soils in the Talas Valley, Northern Kyrgyzstan, in Autumn

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Soil bacterial and fungal communities were investigated in relation to soil type and farm management practices after vegetation harvesting in autumn. Soils from fields cultivated with Phaseolus vulgaris (bean) and Pyrus comminus (pear) and nonarable, natural areas were studied. Microbial diversity was analysed using cultivation-dependent methods (isolation of pure cultures) and cultivation-independent methods (direct extraction of DNA from soil, followed by PCR amplification of the 16S rRNA and 18S rRNA genes). The use of cultivation-dependent methods revealed that there were no differences in ...Daha fazlası

Makale 2023 Ziraat Fakültesi Applied and Environmental Soil Science2023 17 Görüntülenme

In vitro and in vivo screening of bacterial species from contaminated soil for heavy metal biotransformation activity

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

Heavy metals (HMs) are widely used in various industries. High concentrations of HMs can be severely toxic to plants, animals and humans. Microorganism-based bioremediation has shown significant potential in degrading and detoxifying specific HM contaminants. In this study, we cultivated a range of bacterial strains in liquid and solid nutrient medium containing different concentrations of different HMs to select and analyze bacteria capable of transforming HMs. The bacterial strains most resistant to selected HMs and exhibiting the ability to remove HMs from contaminated soils were identified ...Daha fazlası

Makale 2024 Ziraat Fakültesi Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes ( Published online: 27 Apr 2024 ) 0 Görüntülenme

In situ microbial bioremediation of obsolete pesticides at their disposal sites

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

Soil pollution by pesticides is a global problem in many countries, including Kyrgyzstan. Currently, 50 storage facilities for obsolete banned pesticides exist, severely threatening the surrounding populations and environment. In this study, in situ bioremediation technology was used to remove pesticide contamination from soil around the storage facilities. Three conditions were tested: application of fertile soil and bioproduct, application of fertile soil and no bioproduct (Control 1), and application of fertile soil and no bioproduct (Control 2). An aerobic bioremediation approach was used: ...Daha fazlası

Makale 2024 Ziraat Fakültesi Bioremediation Journal ( Published online: 25 Apr 2024 ) 0 Görüntülenme

Biological Control Agents for Crop Protection and Sustainability of Agro-ecosystems in Kyrgyztan

Tinatin DOOLOTKELDİEVA

The main agricultural activities in the Kyrgyz Republic are include the production of cereal grains, vegetables, potatoes and commercial crops such as cotton, tobacco, sugar beets and livestock. The sustainability of the agriculture sector was severely affected by the large scale mono-cropping approach of the Soviet era. Intensive agriculture has caused environmental pollution due to the excessive use of mineral fertilizers and pesticides. To combat this menace we have isolated and introduced non-chemical crop protection agents, the antifungal micro-organisms Streptomyces spp., producing antib ...Daha fazlası

Kitap Bölümü 2013 Ziraat Fakültesi Multifunctional Agriculture, Ecology and Food Security: International Perspectives ( Chapter 14 ) 61 Görüntülenme

Alkane monooxygenase diversity recovered from Central Asia sediments

Tinatin DOOLOTKELDİEVA

Previous studies in our lab and elsewhere showed that different microbial gene groups are obtained from geographically distant sites. Alkane monooxygenases activate terminal carbon-carbon bonds in the first step of alkane degradation with the addition of an oxygen to the compound, resulting in an alcohol product. The aim of this study was to test the hypothesis that unique microbial populations and alkane monooxygenase (alkB and Cytochrome P450) genes can be recovered from geographically distinct sites, with varying degrees of anthropogenic impact. Sediment was collected from six river and str ...Daha fazlası

Kitap Bölümü 2012 Ziraat Fakültesi Diversity of biodegradative gene populations in aquatic sediments examined by gene-targeted metagenomics ( Chapter 3 ) 55 Görüntülenme

Микробные сообщества высокогорных и низкотемпературных почв долины Сон-Куль Кыргызстана

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

С помощью классического метода микробиологии и анализа последовательностей 16SrRNA впервые было изучено количественное соотношение и биоразнообразие микробных комплексов низкотемпературных почв долины Сон-Куль. Структура видового состава бактерий отличалась в почвах под различным покровам растительности по мере возрастания высоты местности. Анализ последовательностей 16SrRNA показал, что в летний период в почвах высокогорной долины Сон-Куль доминирующими филогенетическими группами были Actinobacteria (55,0%), Gammaproteobacteria ( 33,0%) и Firmicutes phyla (22,0%). Видовой состав обнаруженных ...Daha fazlası

Makale 2012 Ziraat Fakültesi Фундаментальные исследования ( 9; Часть 2 ) 79 Görüntülenme

Screening of Wild-Type Fungal Isolates for Cellulolytic Activity

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Background: In this study, wild-type fungal isolates, producing highly effective cellulolytic enzymes were selected for bioconversion of residues and waste from agriculture and rational utilization of energy resources for food production. Methods: We screened wild-type fungal isolates of Aspergillus, Penicillium, and Trichoderma with an enhanced ability to produce extracellular cellulase. We carried out solid-state fermentation on a medium of agricultural waste products, including wheat bran, beet peels, and cotton oil cake, as well as additional sources of nitrogen and mineral elements. Enzym ...Daha fazlası

Makale 2011 Ziraat Fakültesi Microbiology Insights ( 4 ) 74 Görüntülenme

Microbiological Control of Flour-Manufacture: Dissemination of Mycotoxins Producing Fungi in Cereal Products

Tinatin DOOLOTKELDİEVA

Wheat grain and its products are widely consumed as fodder and basic daily food stuffs in Kyrgyzstan. Mycobiota is known to produce hazardous effects to a consumer since it produces mycotoxins. Henceforth, mycobiota starting from the field stage to flour, grain and flour samples were selected for mycological analysis from eight sites of flour manufacture: grain stored in storehouses before milling, mechanically cleaned grain, washed grain, grain dried and prepared for mill, roughly-milled flour, first grade flour and high grade flour. The samples were analyzed using classical mycological and i ...Daha fazlası

Makale 2010 Ziraat Fakültesi Microbiology Insights ( 3 ) 58 Görüntülenme

Detaylı Arama

Filtreler

Koleksiyon [2]

Araştırmacılar [6]

BM Sürdürülebilir Kalkınma [6]

Fakültesi / Enstitütü [2]

Yayın Türü [3]

Yayımlanma Yılı [17]

Dil [2]

Kaynak [28]

Veri Tabanları [19]

İndex Türü [6]

İndex Türü 2 [1]

Yayıncı [17]

Bölümü [2]

Ulusal/Uluslararası [2]

Konu Başlıkları [35]

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Tinatin DOOLOTKELDİEVA

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

Tinatin DOOLOTKELDİEVA

Tinatin DOOLOTKELDİEVA

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Tinatin DOOLOTKELDİEVA