Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.
Keyword: diagnostic PCR; metarhizium anisopliae; metarhizium brunneum; metarhizium robertsii; metarhizum pinghaense; PARB clade; ribosomal intergenic spacer (rIGS); single-marker phylogeny; species delineation
Yayın Adı (dc.title) | Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy |
Yazar/lar (dc.contributor.yazarlar) | Christina Schuster, Yamilé Baró Robaina, Haifa Ben Gharsa, Saikal Bobushova, Romina Guadalupe Manfrino, Alejandra C. Gutierrez, Claudia C. Lopez Lastra, Tinatin Doolotkeldieva, Andreas Leclerque |
Yayın Türü (dc.type) | Makale |
Dil (dc.language) | İngilizce |
Yayımlanma Yılı (dc.date.issued) | 2023 |
Ulusal/Uluslararası (dc.identifier.ulusaluluslararasi) | Uluslararası |
Kaynak (dc.relation.journal) | Journal of Fungi |
Süreli Sayı (dc.identifier.issue) | 10 |
Cilt/Sayı (dc.identifier.volume) | 9 |
Sayfa (dc.identifier.startpage) | Article Number: 996 |
ISSN/ISBN (dc.identifier.issn) | Online ISSN: 2309-608X |
Yayıncı (dc.publisher) | MDPI, Switzerland |
Veri Tabanları (dc.contributor.veritaban) | Web of Science Core Collection |
Veri Tabanları (dc.contributor.veritaban) | MDPI |
Veri Tabanları (dc.contributor.veritaban) | Scopus |
İndex Türü (dc.identifier.index) | SCI Expanded |
İndex Türü (dc.identifier.index) | Scopus |
Etki Faktörü (dc.identifier.etkifaktoru) | 4,7 / 2022-WOS / Son 5 yıl: 5,2 |
Özet (dc.description.abstract) | Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker. |
Özet (dc.description.abstract) | Keyword: diagnostic PCR; metarhizium anisopliae; metarhizium brunneum; metarhizium robertsii; metarhizum pinghaense; PARB clade; ribosomal intergenic spacer (rIGS); single-marker phylogeny; species delineation |
URL (dc.rights) | https://www.mdpi.com/2309-608X/9/10/996 |
DOI (dc.identifier.doi) | 10.3390/jof9100996 |
Fakültesi / Enstitütü (dc.identifier.fakulte) | Ziraat Fakültesi |
Bölümü (dc.identifier.bolum) | Bitki Koruma Bölümü |
Kurumdaki Yazar/lar (dc.contributor.author) | Saykal BOBUŞOVA |
Kurumdaki Yazar/lar (dc.contributor.author) | Tinatin DOOLOTKELDİEVA |
Kayıt No (dc.identifier.kayitno) | BL443F51C2 |
Kayıt Giriş Tarihi (dc.date.available) | 2023-11-09 |
Not (Yayımlanma Yılı) (dc.identifier.notyayinyili) | October 2023 |
Wos No (dc.identifier.wos) | WOS:001092626300001 |
Konu Başlıkları (dc.subject) | diagnostic PCR |
Konu Başlıkları (dc.subject) | metarhizium anisopliae |
Konu Başlıkları (dc.subject) | metarhizium brunneum |
Konu Başlıkları (dc.subject) | metarhizium robertsii |
Konu Başlıkları (dc.subject) | metarhizum pinghaense |
Konu Başlıkları (dc.subject) | PARB clade |
Konu Başlıkları (dc.subject) | ribosomal intergenic spacer (rIGS) |
Konu Başlıkları (dc.subject) | single-marker phylogeny |
Konu Başlıkları (dc.subject) | species delineation |