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Базы данных : Web of Science Core ... ✕Тематический рубрикатор : genotoxicity ✕
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Assessment of Cd-induced genotoxic damage in Urtica pilulifera L. using RAPD-PCR analysis

İlhan DOĞAN

Plants can be used as biological indicators in assessing the damage done by bioaccumulation of heavy metals and their negative impact on the environment. In the present research, Roman nettle (Urtica pilulifera L.) was employed as a bioindicator for cadmium (Cd) pollution. The comparisons between unexposed and exposed plant samples revealed inhibition of the root growth (∼25.96% and ∼45.92% after treatment with 100 and 200 µmol/L Cd concentrations, respectively), reduction in the total soluble protein quantities (∼53.92% and ∼66.29% after treatment with 100 and 200 µmol/L Cd concentrations, re ...Более

Статья 2016 Fen Fakültesi Biotechnology and Biotechnological Equipment30 ( 2 ) 108 Просмотры
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Screening of damage induced by lead (Pb) in rye (Secale cereale L.) – a genetic and physiological approach

İlhan DOĞAN

The fields in which lead (Pb) finds application in the modern world have increased dramatically in recent years. As a consequence of this intensive utilization of Pb, its toxicity tends to pose more and more environmental problems. The aim of this study was to evaluate the genotoxic potential of Pb and to characterize some physiological parameters in Secale cereale under Pb stress. Plants were subjected to different exposure levels of Pb (0, 100, 200 and 400 µmol/L) for two weeks. At the end of the experimental period, the effects of Pb exposure on the photosynthetic pigments content (chloroph ...Более

Статья 2016 Fen Fakültesi Biotechnology and Biotechnological Equipment30 ( 3 ) 55 Просмотры
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Detection of physiological and genotoxic damages reflecting toxicity in Kalanchoe clones

İlhan DOĞAN

In order to make assessments in understanding of physiological and genotoxic effects of imposing cadmium (Cd) on photosynthetic pigment contents along with the changes occurring in genetic material of Kalanchoe plants were used in relation to various Cd-treatments. Young plantlets were originated from a single host plant as clones, and developed in vitro. Developed clones were grown in standard pots with daily watering of Hoagland solution (20 ml) containing different concentrations of cadmium chloride for two months. Cd concentrations of the collected samples were measured by employing ICP-OE ...Более

Статья 2016 Fen Fakültesi Global NEST Journal18 ( 1 ) 101 Просмотры
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Cadmium-induced physiological and genotoxic alterations in Bryophyllum daigremontianum

Zeki SEVEROĞLU

When evaluating the negative impacts of heavy metals in the environment, plants can be used as biological indicators in assessing damage done by bio-accumulation of heavy metals. For detecting the genotoxic effects of heavy metals, the RAPD-PCR technique is applied in plants. In this study, Blyophyllum daigremontianum was used as a bio-indicator for Cadmium (Cd) pollution. B. daigremontianum plantlets were obtained from bulbiliferous spurs. They were grown singly in standard pots and each of the experimental groups of 5 replicates were watered daily with Hoagland solution (20 ml) containing 0, ...Более

Доклады конференции 2013 Fen Fakültesi Proceedings of the International Conference on Environmental Science and Technology 28 Просмотры
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DNA fingerprinting and assessment of some physiological changes in Al-induced Bryophyllum daigremontianum clones

İbrahim İlker ÖZYİĞİT

Aluminum (Al) is one of the most important stress factors that reduce plant productivity in acidic soils. Present work thereby analyzed Al-induced genomic alterations in Bryophyllum daigremontianum clones using RAPD and ISSR markers, and investigated responding changes in photosynthetic pigment (chlorophyll a, b, a/b, total chlorophyll and carotenoid) contents and total soluble protein amounts in plant leaves. The main reason for the use of bulbiferous spurs originated clone plants was to increase reliability and acceptability of RAPD and ISSR techniques in DNA fingerprinting. Raised 40 clone ...Более

Статья 2019 Fen Fakültesi Molecular Biology Reports46 ( 3 ) 65 Просмотры
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Lemna minor, a hyperaccumulator shows elevated levels of Cd accumulation and genomic template stability in binary application of Cd and Ni: a physiological and genetic approach

İbrahim İlker ÖZYİĞİT

In this study, to determine whether having potential to be used as hyperaccumulator for Cd and Ni, numerous experiments were designed for conducting assessments for physiological and genotoxic changes along with defining possible alterations on mineral nutrient status of Lemna minor L. by applying Cd-Ni binary treatments (0, 100, 200 and 400 mu M). Our study revealed that there were increases in the concentrations of B, Cr, Fe, K, Mg, and Mn whereas decreases were noticed in the concentrations of Na and Zn and the levels of Ca were inversely proportional to Cd-Ni applications showing tendency ...Более

Статья 2021 Fen Fakültesi International Journal of Phytoremediation23 ( 12 ) 93 Просмотры
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Cytogenetic and Oxidative Status of Cultured Lymphocytes after Exposure to Extracts from Rhizopogon Mushrooms (Agaricomycetes) In Vitro

Ali ASLAN

In this study, acetone and water extracts obtained from edible mushrooms, Rhizopogon luteolus Fr. and Rh. roseolus (Corda) Th. Fr., containing important bioactive components were used. Total antioxidant capacity (TAC) and total oxidative stress (TOS) levels were examined on human peripheral lymphocyte culture treated with the respective extracts. Levels of genetic damage and cytotoxic effects of the respective extracts on lymphocytes were also tested. In general, when TAC levels of the extracts on cells were examined, a concentration-dependent increase was observed; a negative correlation was ...Более

Статья 2021 Fen Fakültesi International Journal of Medicinal Mushrooms23 ( 3 ) 100 Просмотры
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THE PHYSIOLOGICAL AND DNA DAMAGE RESPONSE OF IN THE LICHEN HYPOGYMNIA PHYSODES TO UV AND HEAVY METAL STRESS

Ali ASLAN

This work aims to determine the response of Hypogymnia physodes (L.) Nyl. (hooded tube lichen) collected in an unpolluted site (Yenice Forest in Karabiik, Turkey) to stress conditions. In the present study, the effect of exposure to different heavy metals (Cd 2, Pb 2, and Cr 6) for different durations and UV radiations dosages on lichen was examined at the physiological and molecular levels. The effects of stress conditions were determined in the case of different parameters concerning heavy metal, protein, chlorophyll, and carotenoid contents and changes in the DNA profiles. According to the ...Более

Статья 2020 Fen Fakültesi Applied Ecology and Environmental Research18 ( 2 ) 37 Просмотры
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