An app for apples: Citizen-led mapping of fire blight in Central Asia

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Fire blight, caused by the bacterial pathogen Erwinia amylovora, is a severe bacterial disease of apple and pear that can quickly destroy whole plants. In the last decade, it was also detected in Central Asia, where wild pomaceous fruit plants represent the dominant species in mid-altitude forests and constitute a critical foundation for the entire ecosystem. Efficiently informing farmers, forestry services and private persons about the instances and dangers of fire blight, the correct way to recognize the symptoms, and the methods of disease control is thus of paramount importance in a vast a ...Более

Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bac ...Более

Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

In Kyrgyzstan, many former storehouses and dump sites for obsolete pesticides exist. In 2009/2010, an inventory and assessment of these sites including risks of environmental hazard has been conducted by FAO and the World Bank. Monitoring revealed high concentration of pesticides listed as persistent organic pollutants (POPs). The purpose of this research was to study the microbial structural complexes of the pesticide-contaminated soils in these dumping zones, and to search for and select microorganism’s destructors with cytochrome P450 genes for pesticide degradation. Culture-dependent and c ...Более

Genetic characterization of Erwinia amylovora isolates detected in the wild walnut-fruit forest of South Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Erwinia amylovora, the causal agent of fire blight, was first reported in Kyrgyz orchards in 2008 and, within a few years, the disease has spread throughout most of the northern part of the country. As fire blight expanded and penetrated new areas, it also reached the unique ecosystem constituted by the Arslonbob wild walnut-fruit forests in South Kyrgyzstan. Here, we present the results of field surveys in the forests and in the nearby orchards and gardens of the Jalal-Abad region during the 2019 season. Further, we use genetic profiling of the CRISPR regions to compare the genotypes of the o ...Более

First report of charcoal rot caused by Macrophomina phaseolina on common bean in Kyrgyzstan

İsmail ERPER | Saykal BOBUŞOVA

Isolation, Identification, and Characterization of the Nematophagous Fungus Arthrobotrys oligospora from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Ayperi MURATBEKOVA

Purpose Predatory fungi have been the subject of fundamental studies and their potential as biological control agents against parasitic plant nematodes has been assessed. The aim of the present study was to isolate and identify predatory fungi, performing in vitro and in vivo screening to select highly active strains to control parasitic nematodes. Methods Different nutrient media were used to isolate predatory fungi and determine their morphological and cultural properties. Identification was performed by classical and molecular biology methods. In vitro and in vivo screening was conducted to ...Более