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Construction, characteristics and high throughput molecular screening methodologies in some special breeding populations: a horticultural perspective

Hasan CAN | İbrahim İlker ÖZYİĞİT | Mustafa PAKSOY

Advanced marker technologies are widely used for evaluation of genetic diversity in cultivated crops, wild ancestors, landraces or any special plant genotypes. Developing agricultural cultivars requires the following steps: (i) determining desired characteristics to be improved, (ii) screening genetic resources to help find a superior cultivar, (iii) intercrossing selected individuals, (iv) generating genetically hybrid populations and screening them for agro-morphological or molecular traits, (v) evaluating the superior cultivar candidates, (vi) testing field performance at different location ...Более

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Heavy Metal Levels and Mineral Nutrient Status of Natural Walnut (Juglans regia L.) Populations in Kyrgyzstan: Nutritional Values of Kernels

Bakıt BORKOYEV | Kalıypa SALİYEVA | Ali Osman SOLAK | İbrahim İlker ÖZYİĞİT

In this study, mineral nutrient and heavy metal (Al, Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn) contents of the walnut kernels and their co-located soil samples collected from the four different zones of natural walnut forests (Sary-Chelek, Arslanbap, and Kara-Alma in Jalal-Abad Region and Kara-Shoro in Osh Region) in Kyrgyzstan were investigated. The highest concentrations for all elements determined in the soil samples were observed in the Sary-Chelek zone whereas the Arslanbap zone was found to be having the lowest concentrations except Fe and Zn. The highest concentrations in the kerne ...Более

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EVALUATION OF ALUMINUM STRESS INDUCED ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES IN ROMAN NETTLE

İbrahim İlker ÖZYİĞİT

In this current work, Roman nettle (Urtica pilulifera L.), a traditional medicinal plant that is very common and widespread species throughout Asia, Europe, and Northern Africa, was used as a model plant to investigate changes in antimicrobial activity following the application of aluminum stress. U pilulifera seedlings were grown in growth-room conditions and 0, 100, and 200 M AlCl3 were applied to the plants together with Hoagland solution (20 ml) for two months. The antimicrobial activities were tested against nine strains of bacteria (Salmonella sp., Staphyllococcus aureus, Escherichia col ...Более

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ECOPHYSIOLOGICAL PROPERTIES OF TURKISH ENDEMIC CENTAUREA CONSANGUINEA DC

İbrahim İlker ÖZYİĞİT

This physiological and ecological study was performed on Centaurea consanguinea DC. to acquire information about certain characteristics (bioclimatic, edaphic, topographic, biotic etc.) of its habitat and distribution in Turkey, determine the requirements for germination, explore the soil-plant relations and obtain data on certain aspects of its population biology. Plant and co-located soil samples were collected from Amasya and Havza District (Samsun) in Turkey using standard methods. Various analyzes were employed for determining of physical, chemical and biological properties of the plant s ...Более

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DNA fingerprinting and assessment of some physiological changes in Al-induced Bryophyllum daigremontianum clones

İbrahim İlker ÖZYİĞİT

Aluminum (Al) is one of the most important stress factors that reduce plant productivity in acidic soils. Present work thereby analyzed Al-induced genomic alterations in Bryophyllum daigremontianum clones using RAPD and ISSR markers, and investigated responding changes in photosynthetic pigment (chlorophyll a, b, a/b, total chlorophyll and carotenoid) contents and total soluble protein amounts in plant leaves. The main reason for the use of bulbiferous spurs originated clone plants was to increase reliability and acceptability of RAPD and ISSR techniques in DNA fingerprinting. Raised 40 clone ...Более

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Comparative analyses of phytochelatin synthase (PCS) genes in higher plants

İbrahim İlker ÖZYİĞİT

Plants employ various defence strategies to ameliorate the effects of heavy metal exposures, leading to re-establishment of metal homeostasis. One of the strategies includes the biosynthesis of main heavy metal detoxifying peptides phytochelatins (PCs) by phytochelatin synthase (PCS). In the present study, 14 PCS homologues were identified in the genomes of 10 selected plants. The size of these PCSs was 452-545 amino acid residues, with characteristic phytochelatin and phytochelatin_C domains. The N-terminal site of the proteins is highly conserved, whereas the C-terminal site is less conserve ...Более

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Abiotic stress-induced regulation of antioxidant genes in different Arabidopsis ecotypes: microarray data evaluation

İbrahim İlker ÖZYİĞİT

Although stresses induce generation of reactive oxygen species (ROS), which are highly reactive and toxic, and cause severe damage to cellular components; plants have very efficient enzymatic ROS-scavenging mechanisms. Despite the substantial knowledge produced about these enzymes, we still have limited knowledge regarding their expression patterns in relation to the stress type, duration and strength. Thus, taking advantage of microarray data, this work evaluated the abiotic stresses (salt, cold, heat and light) induced regulation of six antioxidant enzymes, superoxide dismutase (SOD), catala ...Более

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Construction, characteristics and high throughput molecular screening methodologies in some special breeding populations: a horticultural perspective

Hasan CAN | İbrahim İlker ÖZYİĞİT | Mustafa PAKSOY

Advanced marker technologies are widely used for evaluation of genetic diversity in cultivated crops, wild ancestors, landraces or any special plant genotypes. Developing agricultural cultivars requires the following steps: (i) determining desired characteristics to be improved, (ii) screening genetic resources to help find a superior cultivar, (iii) intercrossing selected individuals, (iv) generating genetically hybrid populations and screening them for agro-morphological or molecular traits, (v) evaluating the superior cultivar candidates, (vi) testing field performance at different location ...Более

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Inhibitory effects of allelopathic crops on seedling growth of weed Hypericum triquetrifolium Turra

İbrahim İlker ÖZYİĞİT

In Laboratory bioassays and Pot culture, we determined the effects of aqueous extracts of 7-allelopathic crops [Alfalfa (Medicago sativa L.), Sorghum (Sorghum bicolor L.), Sunflower (Helianthus annuus L.), Rape (Brassica napus L.), Rye (Secale cereal L.), Barley (Hordeum vulgare L) and Oat (Avena saliva L)] on the seedling growth of weed Hypericum triquetrifolium. In first Lab. bioassay, barley and rape were found most phytotoxic to Hypericum seedlings growth. Then these two crops effects were further tested in pot experiments, with different residues rates (R1, R2, R3 for rape and B1, B2, B3 ...Более

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Heavy Metal Levels and Mineral Nutrient Status in Different Parts of Various Medicinal Plants Collected from Eastern Mediterranean Region of Turkey

İbrahim İlker ÖZYİĞİT

Medicinal plants have been used for treatment of many diseases since the ancient times with traditional knowledge being transferred from generation to generation. However, in recent years, many natural habitats have been contaminated due to increased anthropogenic activities. Plants which are exposed to heavy metal toxicity may experience several serious problems. Furthermore, the inclusion of these plants into the food chain poses a threat to human health as well. Additionally, presence of heavy metals directly effect mineral nutrition and consequently the food quality. The aim of this study ...Более

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