Fabrication of an Electrochemical E. coli Biosensor in Biowells Using Bimetallic Nanoparticle-Labelled Antibodies

Ali Osman SOLAK

An electrochemical biosensor was developed for the determination of Escherichia coli (E. coli) in water. For this purpose, silver-gold core-shell (Ag@Au) bioconjugates and anti-E. coli modified PS-microwells were designed in a sandwich-type format in order to obtain higher sensitivity and selectivity. Ag@Au bimetallic nanoparticles were synthesized by co-reduction method. The core-shell formation was analyzed by using UV-Vis spectroscopy and transmission electron microscopy. Biotin labeled anti-E. coli antibodies were coupled with Ag@Au nanoparticles to form bioconjugates. The electrochemical ...Daha fazlası