Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.
Keyword: diagnostic PCR; metarhizium anisopliae; metarhizium brunneum; metarhizium robertsii; metarhizum pinghaense; PARB clade; ribosomal intergenic spacer (rIGS); single-marker phylogeny; species delineation
Басылманын аты (dc.title) | Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy |
Автор/лор (dc.contributor.yazarlar) | Christina Schuster, Yamilé Baró Robaina, Haifa Ben Gharsa, Saikal Bobushova, Romina Guadalupe Manfrino, Alejandra C. Gutierrez, Claudia C. Lopez Lastra, Tinatin Doolotkeldieva, Andreas Leclerque |
Басылманын түрү (dc.type) | Makale |
Тили (dc.language) | İngilizce |
Жарыяланган жылы (dc.date.issued) | 2023 |
Улуттук/Эл аралык (dc.identifier.ulusaluluslararasi) | Uluslararası |
Булагы (dc.relation.journal) | Journal of Fungi |
Саны (dc.identifier.issue) | 10 |
Том/№ (dc.identifier.volume) | 9 |
Барагы (dc.identifier.startpage) | Article Number: 996 |
ISSN/ISBN (dc.identifier.issn) | Online ISSN: 2309-608X |
Басмаканасы (dc.publisher) | MDPI, Switzerland |
Маалымат базалар (dc.contributor.veritaban) | Web of Science Core Collection |
Маалымат базалар (dc.contributor.veritaban) | MDPI |
Маалымат базалар (dc.contributor.veritaban) | Scopus |
Индекс түрү (dc.identifier.index) | SCI Expanded |
Индекс түрү (dc.identifier.index) | Scopus |
Импакт-фактору (dc.identifier.etkifaktoru) | 4,7 / 2022-WOS / Son 5 yıl: 5,2 |
Қысқаша (dc.description.abstract) | Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker. |
Қысқаша (dc.description.abstract) | Keyword: diagnostic PCR; metarhizium anisopliae; metarhizium brunneum; metarhizium robertsii; metarhizum pinghaense; PARB clade; ribosomal intergenic spacer (rIGS); single-marker phylogeny; species delineation |
URL (dc.rights) | https://www.mdpi.com/2309-608X/9/10/996 |
DOI (dc.identifier.doi) | 10.3390/jof9100996 |
Факультет / Институт (dc.identifier.fakulte) | Ziraat Fakültesi |
Бөлүмү (dc.identifier.bolum) | Bitki Koruma Bölümü |
Мекемедеги автор(лор) (dc.contributor.author) | Saykal BOBUŞOVA |
Мекемедеги автор(лор) (dc.contributor.author) | Tinatin DOOLOTKELDİEVA |
Каттоо № (dc.identifier.kayitno) | BL443F51C2 |
Каттоо киргизүү датасы (dc.date.available) | 2023-11-09 |
Эскертме (Жарыяланган жылы) (dc.identifier.notyayinyili) | October 2023 |
Wos No (dc.identifier.wos) | WOS:001092626300001 |
Предметтик рубрикатор (dc.subject) | diagnostic PCR |
Предметтик рубрикатор (dc.subject) | metarhizium anisopliae |
Предметтик рубрикатор (dc.subject) | metarhizium brunneum |
Предметтик рубрикатор (dc.subject) | metarhizium robertsii |
Предметтик рубрикатор (dc.subject) | metarhizum pinghaense |
Предметтик рубрикатор (dc.subject) | PARB clade |
Предметтик рубрикатор (dc.subject) | ribosomal intergenic spacer (rIGS) |
Предметтик рубрикатор (dc.subject) | single-marker phylogeny |
Предметтик рубрикатор (dc.subject) | species delineation |