BackgroundThis study investigates the effects of cryopreservation and supplementation of Azeri water buffalo's semen with proline (Lp) and fulvic acid (FA). ObjectivesTherefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Lp and FA for buffalo semen cryopreservation. MethodsThirty semen samples of three buffalo bulls were diluted in Tris-egg yolk extender and divided into 12 equal groups including control (C), Lp-10, Lp-20, Lp-40, Lp-60, Lp-80 (containing 10, 20, 40, 60, 80 mM L-proline, respectively), FA-0.2, FA-0.5, FA-0.8, FA-1.1, FA-1.4 and FA-1.7 (containing 0.2, 0.5, 0.8, 1.1, 1.4 and 1.7 fulvic acid, respectively). ResultsThe velocity parameters, TM and PM were improved by FA-1.7, FA-1.4, Lp-40 and Lp-60 groups compared to the C group but no significant difference was found regarding the amplitude of lateral head displacement and straightness compared to the control groups. The percentage of sperm viability and PMF were increased by FA-1.7, FA-1.4, FA-1.1, Lp-40 and Lp-60 groups compared to C group, while in terms of sperm DNA damage FA-1.7, FA-1.4, FA-1.1, Lp-10, Lp-20, Lp-40 and Lp-60 groups showed better results compared to C group. The results also showed that FA-1.7, FA-1.4, FA-1.1, Lp-20, Lp-40 and Lp-60 groups could improve TAC, SOD, GSH and decrease MDA levels. Also, FA-1.7, FA-1.4, Lp-20 and Lp-40 groups could improve GPx levels but just FA-1.7, and Lp-40 groups could improve CAT levels compared to C group. ConclusionsThus, it can be concluded that L-proline and fulvic acid supplementations can improve the quality parameters of post-thawed buffalo bull semen.
Keyword: azari water buffalo; fulvic acid; L-proline; post-thawed semen
Yayın Adı (dc.title) | The influence of L-proline and fulvic acid on oxidative stress and semen quality of buffalo bull semen following cryopreservation |
Yazar/lar (dc.contributor.yazarlar) | Negin Ramazani, Farid Mahd Gharebagh, Ali Soleimanzadeh, Halil Ozancan Arslan, Esin Keles, Desislava Georgieva Gradinarska-Yanakieva, Damla Arslan-Acaröz, Mahdi Zhandi, Alper Baran, Esmail Ayen, Dursun Ali Dinç |
Yayın Türü (dc.type) | Makale |
Dil (dc.language) | İngilizce |
Yayımlanma Yılı (dc.date.issued) | 2023 |
Ulusal/Uluslararası (dc.identifier.ulusaluluslararasi) | Uluslararası |
Kaynak (dc.relation.journal) | Veterinary Medicine and Science |
Süreli Sayı (dc.identifier.issue) | 4 |
Cilt/Sayı (dc.identifier.volume) | 9 |
Sayfa (dc.identifier.startpage) | 1791-1802 |
ISSN/ISBN (dc.identifier.issn) | Online ISSN: 2053-1095 |
Yayıncı (dc.publisher) | Wiley |
Veri Tabanları (dc.contributor.veritaban) | Web of Science Core Collection |
Veri Tabanları (dc.contributor.veritaban) | Wiley |
Veri Tabanları (dc.contributor.veritaban) | Scopus |
İndex Türü (dc.identifier.index) | SCI Expanded |
İndex Türü (dc.identifier.index) | Scopus |
Etki Faktörü (dc.identifier.etkifaktoru) | 1,7 / 2022-WOS / 5 Year: 1,8 |
Özet (dc.description.abstract) | BackgroundThis study investigates the effects of cryopreservation and supplementation of Azeri water buffalo's semen with proline (Lp) and fulvic acid (FA). ObjectivesTherefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Lp and FA for buffalo semen cryopreservation. MethodsThirty semen samples of three buffalo bulls were diluted in Tris-egg yolk extender and divided into 12 equal groups including control (C), Lp-10, Lp-20, Lp-40, Lp-60, Lp-80 (containing 10, 20, 40, 60, 80 mM L-proline, respectively), FA-0.2, FA-0.5, FA-0.8, FA-1.1, FA-1.4 and FA-1.7 (containing 0.2, 0.5, 0.8, 1.1, 1.4 and 1.7 fulvic acid, respectively). ResultsThe velocity parameters, TM and PM were improved by FA-1.7, FA-1.4, Lp-40 and Lp-60 groups compared to the C group but no significant difference was found regarding the amplitude of lateral head displacement and straightness compared to the control groups. The percentage of sperm viability and PMF were increased by FA-1.7, FA-1.4, FA-1.1, Lp-40 and Lp-60 groups compared to C group, while in terms of sperm DNA damage FA-1.7, FA-1.4, FA-1.1, Lp-10, Lp-20, Lp-40 and Lp-60 groups showed better results compared to C group. The results also showed that FA-1.7, FA-1.4, FA-1.1, Lp-20, Lp-40 and Lp-60 groups could improve TAC, SOD, GSH and decrease MDA levels. Also, FA-1.7, FA-1.4, Lp-20 and Lp-40 groups could improve GPx levels but just FA-1.7, and Lp-40 groups could improve CAT levels compared to C group. ConclusionsThus, it can be concluded that L-proline and fulvic acid supplementations can improve the quality parameters of post-thawed buffalo bull semen. |
Özet (dc.description.abstract) | Keyword: azari water buffalo; fulvic acid; L-proline; post-thawed semen |
URL (dc.rights) | https://onlinelibrary.wiley.com/doi/10.1002/vms3.1158 |
DOI (dc.identifier.doi) | 10.1002/vms3.1158 |
Fakültesi / Enstitütü (dc.identifier.fakulte) | Veteriner Fakültesi |
Bölümü (dc.identifier.bolum) | Veteriner |
Kurumdaki Yazar/lar (dc.contributor.author) | Damla ARSLAN ACARÖZ |
Kayıt No (dc.identifier.kayitno) | BL5FFAC58F |
Kayıt Giriş Tarihi (dc.date.available) | 2023-06-02 |
Not (Yayımlanma Yılı) (dc.identifier.notyayinyili) | July 2023 |
Wos No (dc.identifier.wos) | WOS:000989559500001 |
Konu Başlıkları (dc.subject) | azari water buffalo |
Konu Başlıkları (dc.subject) | fulvic acid |
Konu Başlıkları (dc.subject) | L-proline |
Konu Başlıkları (dc.subject) | post-thawed semen |