The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan. The conventional phenotypic (LOPAT, API tests) and PCR-assisted isolation were used for the identificationof Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach (Prunus persica), cherry (Prunus subgen), apricot (Prunus armeniaca), and plum (Prunus salicina) samples taken from the Chy, Issuk-Kul, and Batken regions of the country. 16S rRNA gene amplification was performed with primers 27F (5'-AGA GTT TGA TCC TGG CTC AG -3') and 907R (5 '–CCG TCA ATT CCT TTG AGT TT-3') for the identification of obtained P.syringae pv. syringaе isolates. From 40 primary isolates of Gram-negative rod-shaped bacteria, 12 were identified as Pseudomonas syringae pv. syringae, while the remaining isolates were identified as bacteria from Stenotrophomonas, Xanthomonas, Erwinia genera. The antagonist bio control agent—Streptomyces bacteria strains were screened and selected against the bacterial canker pathogen in in vitro experiments and on apricot seedlings in vivo conditions. Obtained results could encourage to develop a local bio-product based on this bioagent for spraying stone fruits with the initial manifestation of disease symptoms and to conduct preventive treatments in the fall and spring to increase the plant's resistance to pathogens.
Басылманын аты (dc.title) | Characterization of Pseudomonas syringae PV. syringae from diseased stone fruits in Kyrgyzstan and testing of biological agents against pathogen |
Автор/лор (dc.contributor.yazarlar) | Tinatin Doolotkeldieva, Saikal Bobusheva |
Басылманын түрү (dc.type) | Makale |
Тили (dc.language) | İngilizce |
Жарыяланган жылы (dc.date.issued) | 2020 |
Улуттук/Эл аралык (dc.identifier.ulusaluluslararasi) | Uluslararası |
Булагы (dc.relation.journal) | International Journal of Phytopathology |
Саны (dc.identifier.issue) | 2 |
Том/№ (dc.identifier.volume) | 9 |
Барагы (dc.identifier.startpage) | 71-91 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 2313-1241; Online ISSN: 2312-9344 |
Басмаканасы (dc.publisher) | EScience Press, Pakistan |
Маалымат базалар (dc.contributor.veritaban) | Kaynak web sitesi |
Маалымат базалар (dc.contributor.veritaban) | Scopus |
Индекс түрү (dc.identifier.index) | Scopus |
Қысқаша (dc.description.abstract) | The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan. The conventional phenotypic (LOPAT, API tests) and PCR-assisted isolation were used for the identificationof Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach (Prunus persica), cherry (Prunus subgen), apricot (Prunus armeniaca), and plum (Prunus salicina) samples taken from the Chy, Issuk-Kul, and Batken regions of the country. 16S rRNA gene amplification was performed with primers 27F (5'-AGA GTT TGA TCC TGG CTC AG -3') and 907R (5 '–CCG TCA ATT CCT TTG AGT TT-3') for the identification of obtained P.syringae pv. syringaе isolates. From 40 primary isolates of Gram-negative rod-shaped bacteria, 12 were identified as Pseudomonas syringae pv. syringae, while the remaining isolates were identified as bacteria from Stenotrophomonas, Xanthomonas, Erwinia genera. The antagonist bio control agent—Streptomyces bacteria strains were screened and selected against the bacterial canker pathogen in in vitro experiments and on apricot seedlings in vivo conditions. Obtained results could encourage to develop a local bio-product based on this bioagent for spraying stone fruits with the initial manifestation of disease symptoms and to conduct preventive treatments in the fall and spring to increase the plant's resistance to pathogens. |
URL (dc.rights) | https://esciencepress.net/journals/index.php/phytopath/article/view/3270 |
DOI (dc.identifier.doi) | 10.33687/phytopath.009.02.3270 |
Факультет / Институт (dc.identifier.fakulte) | Ziraat Fakültesi |
Бөлүмү (dc.identifier.bolum) | Bitki Koruma Bölümü |
Мекемедеги автор(лор) (dc.contributor.author) | Tinatin DOOLOTKELDİEVA |
Мекемедеги автор(лор) (dc.contributor.author) | Saykal BOBUŞOVA |
Каттоо № (dc.identifier.kayitno) | BL0136A87D |
Каттоо киргизүү датасы (dc.date.available) | 2023-03-02 |
Эскертме (Жарыяланган жылы) (dc.identifier.notyayinyili) | 2020 |
Предметтик рубрикатор (dc.subject) | stone fruits |
Предметтик рубрикатор (dc.subject) | bacterial cancer |
Предметтик рубрикатор (dc.subject) | pseudomonas syringa pv. syringaе |
Предметтик рубрикатор (dc.subject) | bio control agent |
Предметтик рубрикатор (dc.subject) | streptomyces bacteria |