This study reports direct electron transfer (DET) from immobilized glucose oxidase (GOx) via grafted and electropolymerized 1,10-phenanthroline monohydrate (PMH). The layer of poly-1,10-phenanthroline (PPMH) was gained via electrochemical deposition, which was used to create the PPMH-modified GC-electrode (PPMH/GC-electrode). Further, the GOx was immobilized on the PPMH/GC-electrode. The effect of surface-modification by the PPMH on the electron-transfer between enzyme and electrode-surface and some other electrochemical/analytical-parameters of newly designed enzymatic-electrode were evaluated. The PPMH/GC-electrode showed superior DET to/from flavine adenine dinucleotide cofactor of GOx, while some redox-compounds including ferrocene and K3[Fe(CN)6] were completely electrochemically inactive on the PPMH/GC-electrode. It was also found that the resulting GOx/PPMH/GC-electrode functioned as a “direct response type” glucose-biosensor. The biosensor showed excellent selectivity towards glucose and demonstrated good operational-stability. According to our best knowledge, this study is the first scientific report on electrochemical-polymerization of PMH on the GC-electrode in non-aqueous media followed by its application in the design of glucose-biosensor. -
Keywords: Surface-modification; Phenanthroline; Glucose oxidase; Biosensor; Electron transfer; Electrocatalysis
Publication Name (dc.title) | Direct electron transfer from glucose oxidase immobilized on polyphenanthroline-modified glassy carbon electrode |
Author/s (dc.contributor.yazarlar) | Yasemin Oztekin, Almira Ramanaviciene, Zafer Yazicigil, Ali Osman Solak, Arunas Ramanavicius |
Publication type (dc.type) | Makale |
Language (dc.language) | İngilizce |
Publication year (dc.date.issued) | 2011 |
National/International (dc.identifier.ulusaluluslararasi) | Uluslararası |
Source (dc.relation.journal) | Biosensors and Bioelectronics |
Number (dc.identifier.issue) | 5 |
Volume/Issue (dc.identifier.volume) | 26 |
Page (dc.identifier.startpage) | 2541–2546 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 0956-5663 |
Publisher (dc.publisher) | Elsevier |
Databases (dc.contributor.veritaban) | Web of Science Core Collection |
Databases (dc.contributor.veritaban) | Sciencedirect |
Databases (dc.contributor.veritaban) | Scopus |
Index Type (dc.identifier.index) | SCI Expanded |
Index Type (dc.identifier.index) | Scopus |
Impact Factor (dc.identifier.etkifaktoru) | 8,173 / 2017-WOS / 5 Year: 7,291 |
Abstract (dc.description.abstract) | This study reports direct electron transfer (DET) from immobilized glucose oxidase (GOx) via grafted and electropolymerized 1,10-phenanthroline monohydrate (PMH). The layer of poly-1,10-phenanthroline (PPMH) was gained via electrochemical deposition, which was used to create the PPMH-modified GC-electrode (PPMH/GC-electrode). Further, the GOx was immobilized on the PPMH/GC-electrode. The effect of surface-modification by the PPMH on the electron-transfer between enzyme and electrode-surface and some other electrochemical/analytical-parameters of newly designed enzymatic-electrode were evaluated. The PPMH/GC-electrode showed superior DET to/from flavine adenine dinucleotide cofactor of GOx, while some redox-compounds including ferrocene and K3[Fe(CN)6] were completely electrochemically inactive on the PPMH/GC-electrode. It was also found that the resulting GOx/PPMH/GC-electrode functioned as a “direct response type” glucose-biosensor. The biosensor showed excellent selectivity towards glucose and demonstrated good operational-stability. According to our best knowledge, this study is the first scientific report on electrochemical-polymerization of PMH on the GC-electrode in non-aqueous media followed by its application in the design of glucose-biosensor. - |
Abstract (dc.description.abstract) | Keywords: Surface-modification; Phenanthroline; Glucose oxidase; Biosensor; Electron transfer; Electrocatalysis |
URL (dc.rights) | http://www.sciencedirect.com/science/article/pii/S0956566310007621 |
DOI (dc.identifier.doi) | 10.1016/j.bios.2010.11.001 |
Faculty / Institute (dc.identifier.fakulte) | Mühendislik Fakültesi |
Department (dc.identifier.bolum) | Kimya Mühendisliği Bölümü |
Author(s) in the Institution (dc.contributor.author) | Ali Osman SOLAK |
Kayıt No (dc.identifier.kayitno) | BLCB20CD1D |
Record Add Date (dc.date.available) | 2016-03-03 |
Notes (Publication year) (dc.identifier.notyayinyili) | January 2011 |
Wos No (dc.identifier.wos) | WOS:000286904400121 |
Subject Headings (dc.subject) | surface-modification |
Subject Headings (dc.subject) | phenanthroline |
Subject Headings (dc.subject) | glucose oxidase |
Subject Headings (dc.subject) | biosensor |
Subject Headings (dc.subject) | electron transfer |
Subject Headings (dc.subject) | electrocatalysis |