When evaluating the negative impacts of heavy metals in the environment, plants can be used as biological indicators in assessing damage done by bio-accumulation of heavy metals. For detecting the genotoxic effects of heavy metals, the RAPD-PCR technique is applied in plants. In this study, Blyophyllum daigremontianum was used as a bio-indicator for Cadmium (Cd) pollution. B. daigremontianum plantlets were obtained from bulbiliferous spurs. They were grown singly in standard pots and each of the experimental groups of 5 replicates were watered daily with Hoagland solution (20 ml) containing 0, 50, 100, 200 and 400 mu M Cd for two months. Plantlets were harvested at the end of the two-month experimental period and photosynthetic pigment (chlorophyll a, chlorophyll b, total chlorophyll, chlorophyll a/b and carotenoids) contents of B. daigremontianum in response to Cd stress were investigated. The comparison between unexposed and exposed B. daigremontianum groups revealed gradual reductions of photosynthetic pigment contents (-40.57% for chlorophyll a, -37.63% for chlorophyll b, -36.27% for total chlorophyll, -20.58% for chlorophyll a/b and -37.66% for carotenoids) at 400 pM and a genomic instability as Cd concentration increases. The results indicated that changes in RAPD-PCR profiles following Cd treatments included a variation in band intensities, a loss of normal bands, and the appearance of new bands compared to unexposed plantlets. Cd concentrations of the collected samples were measured by employing ICP-OES during the study. The concentrations of Cd at 400 mu M Cd treatment were increased significantly in leaves (-5.03 fold), stems (-6.28 fold) and roots (-3.90 fold) of B. daigremontianum. The present study evidences that as an investigation tool for environmental toxicology, the RAPD technique can be useful and can be applied as a suitable biomarker assay for the detection of genotoxic effects of Cd pollution on plants.
Publication Name (dc.title) | Cadmium-induced physiological and genotoxic alterations in Bryophyllum daigremontianum |
Author/s (dc.contributor.yazarlar) | Ibrahim Ilker Ozyigit, Secil Yilmaz, Ilhan Dogan, Mehmet Serdal Sakcali, Guzin Kekec, Goksel Demir, Zeki Severoglu |
Publication type (dc.type) | Konferans Bildirisi |
Language (dc.language) | İngilizce |
Publication year (dc.date.issued) | 2013 |
National/International (dc.identifier.ulusaluluslararasi) | Uluslararası |
Source (dc.relation.journal) | Proceedings of the International Conference on Environmental Science and Technology |
Additional source name / Conference information (dc.identifier.kaynakadiekbilgi) | Proceedings of the 13th International Conference on Environmental Science and Technology (CEST) |
Additional source name / Conference information (dc.identifier.kaynakadiekbilgi) | 5-7 September 2013, Athens, Greece |
ISSN/ISBN (dc.identifier.issn) | ISBN: 978-960-7475-51-0; ISSN: 1106-5516 |
Publisher (dc.publisher) | Global Network for Environmental Science and Technology (Global NEST). University of the Aegean |
Databases (dc.contributor.veritaban) | Web of Science Core Collection |
Databases (dc.contributor.veritaban) | Kaynak web sitesi |
Index Type (dc.identifier.index) | CPCI-S |
Abstract (dc.description.abstract) | When evaluating the negative impacts of heavy metals in the environment, plants can be used as biological indicators in assessing damage done by bio-accumulation of heavy metals. For detecting the genotoxic effects of heavy metals, the RAPD-PCR technique is applied in plants. In this study, Blyophyllum daigremontianum was used as a bio-indicator for Cadmium (Cd) pollution. B. daigremontianum plantlets were obtained from bulbiliferous spurs. They were grown singly in standard pots and each of the experimental groups of 5 replicates were watered daily with Hoagland solution (20 ml) containing 0, 50, 100, 200 and 400 mu M Cd for two months. Plantlets were harvested at the end of the two-month experimental period and photosynthetic pigment (chlorophyll a, chlorophyll b, total chlorophyll, chlorophyll a/b and carotenoids) contents of B. daigremontianum in response to Cd stress were investigated. The comparison between unexposed and exposed B. daigremontianum groups revealed gradual reductions of photosynthetic pigment contents (-40.57% for chlorophyll a, -37.63% for chlorophyll b, -36.27% for total chlorophyll, -20.58% for chlorophyll a/b and -37.66% for carotenoids) at 400 pM and a genomic instability as Cd concentration increases. The results indicated that changes in RAPD-PCR profiles following Cd treatments included a variation in band intensities, a loss of normal bands, and the appearance of new bands compared to unexposed plantlets. Cd concentrations of the collected samples were measured by employing ICP-OES during the study. The concentrations of Cd at 400 mu M Cd treatment were increased significantly in leaves (-5.03 fold), stems (-6.28 fold) and roots (-3.90 fold) of B. daigremontianum. The present study evidences that as an investigation tool for environmental toxicology, the RAPD technique can be useful and can be applied as a suitable biomarker assay for the detection of genotoxic effects of Cd pollution on plants. |
URL (dc.rights) | http://www.gnest.org/proceedings/cest2013/public_html/papers/0492.pdf |
Faculty / Institute (dc.identifier.fakulte) | Fen Fakültesi |
Department (dc.identifier.bolum) | Biyoloji Bölümü |
Author(s) in the Institution (dc.contributor.author) | Zeki SEVEROĞLU |
Kayıt No (dc.identifier.kayitno) | BLD9B0586F |
Record Add Date (dc.date.available) | 2017-04-04 |
Notes (Publication year) (dc.identifier.notyayinyili) | 05-07 September 2013 |
Wos No (dc.identifier.wos) | WOS:000346067900278 |
Subject Headings (dc.subject) | kalanchoe daigremontiana |
Subject Headings (dc.subject) | RAPD-PCR |
Subject Headings (dc.subject) | genotoxicity |
Subject Headings (dc.subject) | heavy metals |