A highly sensitive method for detection of DNA hybridization was developed. This method was based on the modification of glassy carbon electrode with gold nanoparticles (AuNPs) involving p-aminothiophenol (ATP) functionalized graphene oxide (GO). This GO was used as a platform for impedimetric genosensing using 5′-TA GGG CCA CTT GGA CCT-(CH2)3-SH-3′ single-stranded probe (ss-DNA), 5′-AGG TCC AAG TGG CCC TA-3′ (target DNA), 5′-SH-C6-TAG GGC CA-3′ (non-complementary-1) and 5′-SH-C6-TGC CCG TTA CG 3-′ (non-complementary-2) oligonucleotide sequences. The film exhibited excellent properties for immobilizing DNA probes and sensing DNA hybridization. The DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), and found that the charge transfer resistance (Rct) of the electrode increased with the concentration of the target DNA hybridized with the ss-DNA. The linear detection range was from 1.0 × 10−13 M to 1.0 × 10−7 M and the detection limit was 1.10 × 10−14 M (n = 6). Compared with the other electrochemical DNA biosensors, the proposed biosensor showed its own performance of simplicity, good stability, and high sensitivity. -
Keywords: Graphene oxide; Gold nanoparticles; Electrochemical impedance spectroscopy; DNA nanobiosensor
Publication Name (dc.title) | A novel impedimetric biosensor based on graphene oxide/gold nanoplatform for detection of DNA arrays |
Author/s (dc.contributor.yazarlar) | Vinod Kumar Gupta, Mehmet Lütfi Yola, Munewar Saeed Qureshi, Ali Osman Solak, Necip Atar, Zafer Üstündağ |
Publication type (dc.type) | Makale |
Language (dc.language) | İngilizce |
Publication year (dc.date.issued) | 2013 |
National/International (dc.identifier.ulusaluluslararasi) | Uluslararası |
Source (dc.relation.journal) | Sensors and Actuators B: Chemical |
Volume/Issue (dc.identifier.volume) | 188 |
Page (dc.identifier.startpage) | 1201–1211 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 0925-4005 |
Publisher (dc.publisher) | Elsevier |
Databases (dc.contributor.veritaban) | Web of Science Core Collection |
Databases (dc.contributor.veritaban) | Sciencedirect |
Databases (dc.contributor.veritaban) | Scopus |
Index Type (dc.identifier.index) | SCI Expanded |
Index Type (dc.identifier.index) | Scopus |
Impact Factor (dc.identifier.etkifaktoru) | 5,667 / 2017-WOS / 5 Year: 5,118 |
Abstract (dc.description.abstract) | A highly sensitive method for detection of DNA hybridization was developed. This method was based on the modification of glassy carbon electrode with gold nanoparticles (AuNPs) involving p-aminothiophenol (ATP) functionalized graphene oxide (GO). This GO was used as a platform for impedimetric genosensing using 5′-TA GGG CCA CTT GGA CCT-(CH2)3-SH-3′ single-stranded probe (ss-DNA), 5′-AGG TCC AAG TGG CCC TA-3′ (target DNA), 5′-SH-C6-TAG GGC CA-3′ (non-complementary-1) and 5′-SH-C6-TGC CCG TTA CG 3-′ (non-complementary-2) oligonucleotide sequences. The film exhibited excellent properties for immobilizing DNA probes and sensing DNA hybridization. The DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), and found that the charge transfer resistance (Rct) of the electrode increased with the concentration of the target DNA hybridized with the ss-DNA. The linear detection range was from 1.0 × 10−13 M to 1.0 × 10−7 M and the detection limit was 1.10 × 10−14 M (n = 6). Compared with the other electrochemical DNA biosensors, the proposed biosensor showed its own performance of simplicity, good stability, and high sensitivity. - |
Abstract (dc.description.abstract) | Keywords: Graphene oxide; Gold nanoparticles; Electrochemical impedance spectroscopy; DNA nanobiosensor |
URL (dc.rights) | http://www.sciencedirect.com/science/article/pii/S0925400513009581 |
DOI (dc.identifier.doi) | 10.1016/j.snb.2013.08.034 |
Faculty / Institute (dc.identifier.fakulte) | Mühendislik Fakültesi |
Department (dc.identifier.bolum) | Kimya Mühendisliği Bölümü |
Author(s) in the Institution (dc.contributor.author) | Ali Osman SOLAK |
Kayıt No (dc.identifier.kayitno) | BL554CFB4C |
Record Add Date (dc.date.available) | 2016-03-17 |
Notes (Publication year) (dc.identifier.notyayinyili) | November 2013 |
Wos No (dc.identifier.wos) | WOS:000326345600161 |
Subject Headings (dc.subject) | graphene oxide |
Subject Headings (dc.subject) | gold nanoparticles |
Subject Headings (dc.subject) | electrochemical impedance spectroscopy |
Subject Headings (dc.subject) | DNA nanobiosensor |