A highly sensitive method for detection of DNA hybridization was developed. This method was based on the modification of glassy carbon electrode with gold nanoparticles (AuNPs) involving p-aminothiophenol (ATP) functionalized graphene oxide (GO). This GO was used as a platform for impedimetric genosensing using 5′-TA GGG CCA CTT GGA CCT-(CH2)3-SH-3′ single-stranded probe (ss-DNA), 5′-AGG TCC AAG TGG CCC TA-3′ (target DNA), 5′-SH-C6-TAG GGC CA-3′ (non-complementary-1) and 5′-SH-C6-TGC CCG TTA CG 3-′ (non-complementary-2) oligonucleotide sequences. The film exhibited excellent properties for immobilizing DNA probes and sensing DNA hybridization. The DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), and found that the charge transfer resistance (Rct) of the electrode increased with the concentration of the target DNA hybridized with the ss-DNA. The linear detection range was from 1.0 × 10−13 M to 1.0 × 10−7 M and the detection limit was 1.10 × 10−14 M (n = 6). Compared with the other electrochemical DNA biosensors, the proposed biosensor showed its own performance of simplicity, good stability, and high sensitivity. -
Keywords: Graphene oxide; Gold nanoparticles; Electrochemical impedance spectroscopy; DNA nanobiosensor
Название публикации (dc.title) | A novel impedimetric biosensor based on graphene oxide/gold nanoplatform for detection of DNA arrays |
Автор/ы (dc.contributor.yazarlar) | Vinod Kumar Gupta, Mehmet Lütfi Yola, Munewar Saeed Qureshi, Ali Osman Solak, Necip Atar, Zafer Üstündağ |
Вид публикации (dc.type) | Makale |
Язык (dc.language) | İngilizce |
Год публикации (dc.date.issued) | 2013 |
Национальный/Международный (dc.identifier.ulusaluluslararasi) | Uluslararası |
Источник (dc.relation.journal) | Sensors and Actuators B: Chemical |
Том/№ (dc.identifier.volume) | 188 |
Страница (dc.identifier.startpage) | 1201–1211 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 0925-4005 |
Издатель (dc.publisher) | Elsevier |
Базы данных (dc.contributor.veritaban) | Web of Science Core Collection |
Базы данных (dc.contributor.veritaban) | Sciencedirect |
Базы данных (dc.contributor.veritaban) | Scopus |
Вид индекса (dc.identifier.index) | SCI Expanded |
Вид индекса (dc.identifier.index) | Scopus |
Импакт-фактор (dc.identifier.etkifaktoru) | 5,667 / 2017-WOS / 5 Year: 5,118 |
Резюме (dc.description.abstract) | A highly sensitive method for detection of DNA hybridization was developed. This method was based on the modification of glassy carbon electrode with gold nanoparticles (AuNPs) involving p-aminothiophenol (ATP) functionalized graphene oxide (GO). This GO was used as a platform for impedimetric genosensing using 5′-TA GGG CCA CTT GGA CCT-(CH2)3-SH-3′ single-stranded probe (ss-DNA), 5′-AGG TCC AAG TGG CCC TA-3′ (target DNA), 5′-SH-C6-TAG GGC CA-3′ (non-complementary-1) and 5′-SH-C6-TGC CCG TTA CG 3-′ (non-complementary-2) oligonucleotide sequences. The film exhibited excellent properties for immobilizing DNA probes and sensing DNA hybridization. The DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), and found that the charge transfer resistance (Rct) of the electrode increased with the concentration of the target DNA hybridized with the ss-DNA. The linear detection range was from 1.0 × 10−13 M to 1.0 × 10−7 M and the detection limit was 1.10 × 10−14 M (n = 6). Compared with the other electrochemical DNA biosensors, the proposed biosensor showed its own performance of simplicity, good stability, and high sensitivity. - |
Резюме (dc.description.abstract) | Keywords: Graphene oxide; Gold nanoparticles; Electrochemical impedance spectroscopy; DNA nanobiosensor |
URL (dc.rights) | http://www.sciencedirect.com/science/article/pii/S0925400513009581 |
DOI (dc.identifier.doi) | 10.1016/j.snb.2013.08.034 |
Факультет / Институт (dc.identifier.fakulte) | Mühendislik Fakültesi |
Кафедра (dc.identifier.bolum) | Kimya Mühendisliği Bölümü |
Автор(ы) в учреждении (dc.contributor.author) | Ali Osman SOLAK |
№ регистрации (dc.identifier.kayitno) | BL554CFB4C |
Дата регистрации (dc.date.available) | 2016-03-17 |
Заметка (Год публикации) (dc.identifier.notyayinyili) | November 2013 |
Wos No (dc.identifier.wos) | WOS:000326345600161 |
Тематический рубрикатор (dc.subject) | graphene oxide |
Тематический рубрикатор (dc.subject) | gold nanoparticles |
Тематический рубрикатор (dc.subject) | electrochemical impedance spectroscopy |
Тематический рубрикатор (dc.subject) | DNA nanobiosensor |