Identification and Prevalence of Ralstonia Solanacearum From Potato Fields of Kyrgyzstan

For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed during storage. For ELISA was used Ralstonia solanacerium PathoScreen R Kit DAS ELISA (Agdia product, USA).Isolated races of Ralstonia solanacearum by biochemical characteristics were classified as a 3-biotype. -

Keywords: The Potato (Solanum tuberosum) Cultivars, Identification of Ralstonia Solanacearum , ELISA assay, Biochemical Tests.

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Yayın Adı
(dc.title)
Identification and Prevalence of Ralstonia Solanacearum From Potato Fields of Kyrgyzstan
Yazar/lar
(dc.contributor.yazarlar)
Tinatin Döölotkeldieva, Saykal Bobuşeva
Yayın Türü
(dc.type)
Makale
Dil
(dc.language)
İngilizce
Yayımlanma Yılı
(dc.date.issued)
2014
Ulusal/Uluslararası
(dc.identifier.ulusaluluslararasi)
Ulusal
Kaynak
(dc.relation.journal)
MANAS Journal of Agriculture, Veterinary and Life Science (MJAVL)
Süreli Sayı
(dc.identifier.issue)
1
Cilt/Sayı
(dc.identifier.volume)
4
Sayfa
(dc.identifier.startpage)
1-9
ISSN/ISBN
(dc.identifier.issn)
Online ISSN: 1694-7932
Yayıncı
(dc.publisher)
Kyrgyz - Turkish Manas University
Veri Tabanları
(dc.contributor.veritaban)
Ulakbim (DergiPark)
İndex Türü
(dc.identifier.index)
Diğer Hakemli Dergi
Özet
(dc.description.abstract)
For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed during storage. For ELISA was used Ralstonia solanacerium PathoScreen R Kit DAS ELISA (Agdia product, USA).Isolated races of Ralstonia solanacearum by biochemical characteristics were classified as a 3-biotype. -
Özet
(dc.description.abstract)
Keywords: The Potato (Solanum tuberosum) Cultivars, Identification of Ralstonia Solanacearum , ELISA assay, Biochemical Tests.
URL
(dc.rights)
https://dergipark.org.tr/tr/pub/mjavl/issue/40468/484814
Fakültesi / Enstitütü
(dc.identifier.fakulte)
Ziraat Fakültesi
Bölümü
(dc.identifier.bolum)
Bitki Koruma Bölümü
Kurumdaki Yazar/lar
(dc.contributor.author)
Tinatin DOOLOTKELDİEVA
Kurumdaki Yazar/lar
(dc.contributor.author)
Saykal BOBUŞOVA
Kayıt No
(dc.identifier.kayitno)
BLBF0AB0AA
Kayıt Giriş Tarihi
(dc.date.available)
2016-07-15
Not (Yayımlanma Yılı)
(dc.identifier.notyayinyili)
2014
Konu Başlıkları
(dc.subject)
the potato (solanum tuberosum) cultivars
Konu Başlıkları
(dc.subject)
identification of ralstonia solanacearum
Konu Başlıkları
(dc.subject)
elisa assay
Konu Başlıkları
(dc.subject)
biochemical tests
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