In Kyrgyzstan, many former storehouses and dump sites for obsolete pesticides exist. In 2009/2010, an inventory and assessment of these sites including risks of environmental hazard has been conducted by FAO and the World Bank. Monitoring revealed high concentration of pesticides listed as persistent organic pollutants (POPs). The purpose of this research was to study the microbial structural complexes of the pesticide-contaminated soils in these dumping zones, and to search for and select microorganism’s destructors with cytochrome P450 genes for pesticide degradation. Culture-dependent and culture-independent approaches were used to determine the taxonomic composition of these bacterial communities. The universal primer set for the 16S ribosomal RNA (rRNA) gene and the specific primer set P450R were used to amplify the cytochrome P450 hydroxylase gene. In soils from Suzak A and B and soils from Balykchy dumping sites, the bacteria from the Actinobacteria phylum (Micrococcus genus) were dominant. These bacteria made up 32–47?f the indigenous local microflora; bacteria species from the Pseudomonas genus (Gammaproteobacteria phylum) made up 23?n Suzak, 12?n Balykchy soils. Bacillus species from the Firmicutes phylum were found only in Suzak soils. The 16S rRNA analyses and the specific primer set P450R have revealed bacteria with cytochrome genes which are directly involved in the degradation process of organic carbon compounds. Experiments were carried out to help select active degraders from the bacterial populations isolated and used to degrade Aldrin in laboratory. Active bacterial strains from the Pseudomonas fluorescens and Bacillus polymyxa population were selected which demonstrated high rates of degradation activity on Aldrin
Басылманын аты (dc.title) | Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities |
Автор/лор (dc.contributor.yazarlar) | Tinatin Doolotkeldieva, Maxabat Konurbaeva, Saykal Bobusheva |
Басылманын түрү (dc.type) | Makale |
Тили (dc.language) | İngilizce |
Жарыяланган жылы (dc.date.issued) | 2018 |
Улуттук/Эл аралык (dc.identifier.ulusaluluslararasi) | Uluslararası |
Булагы (dc.relation.journal) | Environmental Science and Pollution Research |
Саны (dc.identifier.issue) | 32 |
Том/№ (dc.identifier.volume) | 25 |
Барагы (dc.identifier.startpage) | 31848–31862 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 0944-1344; Online ISSN: 1614-7499 |
Басмаканасы (dc.publisher) | Springer |
Маалымат базалар (dc.contributor.veritaban) | Web of Science Core Collection |
Маалымат базалар (dc.contributor.veritaban) | Springer |
Маалымат базалар (dc.contributor.veritaban) | Scopus |
Индекс түрү (dc.identifier.index) | SCI Expanded |
Индекс түрү (dc.identifier.index) | Scopus |
Импакт-фактору (dc.identifier.etkifaktoru) | 2,8 / 2017-WOS / 5 Year: 2,989 |
Қысқаша (dc.description.abstract) | In Kyrgyzstan, many former storehouses and dump sites for obsolete pesticides exist. In 2009/2010, an inventory and assessment of these sites including risks of environmental hazard has been conducted by FAO and the World Bank. Monitoring revealed high concentration of pesticides listed as persistent organic pollutants (POPs). The purpose of this research was to study the microbial structural complexes of the pesticide-contaminated soils in these dumping zones, and to search for and select microorganism’s destructors with cytochrome P450 genes for pesticide degradation. Culture-dependent and culture-independent approaches were used to determine the taxonomic composition of these bacterial communities. The universal primer set for the 16S ribosomal RNA (rRNA) gene and the specific primer set P450R were used to amplify the cytochrome P450 hydroxylase gene. In soils from Suzak A and B and soils from Balykchy dumping sites, the bacteria from the Actinobacteria phylum (Micrococcus genus) were dominant. These bacteria made up 32–47?f the indigenous local microflora; bacteria species from the Pseudomonas genus (Gammaproteobacteria phylum) made up 23?n Suzak, 12?n Balykchy soils. Bacillus species from the Firmicutes phylum were found only in Suzak soils. The 16S rRNA analyses and the specific primer set P450R have revealed bacteria with cytochrome genes which are directly involved in the degradation process of organic carbon compounds. Experiments were carried out to help select active degraders from the bacterial populations isolated and used to degrade Aldrin in laboratory. Active bacterial strains from the Pseudomonas fluorescens and Bacillus polymyxa population were selected which demonstrated high rates of degradation activity on Aldrin |
URL (dc.rights) | https://link.springer.com/article/10.1007/s11356-017-0048-5 |
DOI (dc.identifier.doi) | 10.1007/s11356-017-0048-5 |
Факультет / Институт (dc.identifier.fakulte) | Ziraat Fakültesi |
Бөлүмү (dc.identifier.bolum) | Bitki Koruma Bölümü |
Мекемедеги автор(лор) (dc.contributor.author) | Tinatin DOOLOTKELDİEVA |
Мекемедеги автор(лор) (dc.contributor.author) | Mahabat KONURBAYEVA |
Мекемедеги автор(лор) (dc.contributor.author) | Saykal BOBUŞOVA |
Каттоо № (dc.identifier.kayitno) | BL207C754B |
Каттоо киргизүү датасы (dc.date.available) | 2017-10-10 |
Эскертме (Жарыяланган жылы) (dc.identifier.notyayinyili) | November 2018 |
Wos No (dc.identifier.wos) | WOS:000448662700007 |
Предметтик рубрикатор (dc.subject) | obsolete pesticide dump |
Предметтик рубрикатор (dc.subject) | contaminated soil |
Предметтик рубрикатор (dc.subject) | microbial consortia |
Предметтик рубрикатор (dc.subject) | bacterial populations with P450 cytochrome genes |
Предметтик рубрикатор (dc.subject) | active bacteria for aerobic bioremediation of pesticides |