We report on the enzootic foci of the insect pathogenic fungus, Beauveria bassiana, found in high meadows in the middle mountain steppes of Kyrgyzstan, at elevations from 1000 m to 2200 m. The growth characteristics of various B. bassiana isolates on different media and as a function of temperature were studied. In addition, the ability of the fungal isolates to produce enzymes with amylase, protease and lipase activities was investigated. Dense biomass production on inexpensive solid media (oatmeal and bean oil meal) produced conidia used for insect bioassays targeting white grub larvae (Phyllophaga fullo, Coleoptera, Scarabaeidae) and nymphal and adult populations of whiteflies (Trialeurodes vaporariorum, Hemiptera, Aleyrodidae). The efficacies of the tested B. bassiana strains for third instar white grub larvae varied, with only two strains showing high entomopathogenic activity. At 25 degrees C, mortality reached 73?or Bay.5-Gal and 74?or Bay.1-Lep at 55 d post-infection, but was lower, 27?nd 29?respectively, at 12 degrees C. These two strains produced significantly higher mortality in adult and whitefly nymphs, with 65-75?ortality 6 d post-infection. Based on morphological characters, including production of ellipsoidal conidia, and molecular characters (ITS, partial 18S (SSU rDNA) and EF1-alpha sequences), the isolates were identified as Beauveria bassiana belonging to Clade E from Asia. Our results add to data on the diversity of ecosystems inhabited by B. bassiana and provide a local resource for pest control efforts.
Басылманын аты (dc.title) | Characterization of Beauveria bassiana isolates from Kyrgyzstan |
Автор/лор (dc.contributor.yazarlar) | Tinatin Doolotkeldieva, Saikal Bobusheva, Aijamal Kulmanbetova, Sezim Zholdoshbekova, Aygerim Amanbek Kyzy |
Басылманын түрү (dc.type) | Makale |
Тили (dc.language) | İngilizce |
Жарыяланган жылы (dc.date.issued) | 2019 |
Улуттук/Эл аралык (dc.identifier.ulusaluluslararasi) | Uluslararası |
Булагы (dc.relation.journal) | Journal of Invertebrate Pathology |
Том/№ (dc.identifier.volume) | 167 |
Барагы (dc.identifier.startpage) | Article Number: 107243 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 0022-2011; Online ISSN: 1096-0805 |
Басмаканасы (dc.publisher) | Elsevier |
Маалымат базалар (dc.contributor.veritaban) | Web of Science Core Collection |
Маалымат базалар (dc.contributor.veritaban) | Sciencedirect |
Маалымат базалар (dc.contributor.veritaban) | Scopus |
Индекс түрү (dc.identifier.index) | SCI Expanded |
Индекс түрү (dc.identifier.index) | Scopus |
Импакт-фактору (dc.identifier.etkifaktoru) | 2,101 / 2018-WOS / 5 Year: 2,535 |
Қысқаша (dc.description.abstract) | We report on the enzootic foci of the insect pathogenic fungus, Beauveria bassiana, found in high meadows in the middle mountain steppes of Kyrgyzstan, at elevations from 1000 m to 2200 m. The growth characteristics of various B. bassiana isolates on different media and as a function of temperature were studied. In addition, the ability of the fungal isolates to produce enzymes with amylase, protease and lipase activities was investigated. Dense biomass production on inexpensive solid media (oatmeal and bean oil meal) produced conidia used for insect bioassays targeting white grub larvae (Phyllophaga fullo, Coleoptera, Scarabaeidae) and nymphal and adult populations of whiteflies (Trialeurodes vaporariorum, Hemiptera, Aleyrodidae). The efficacies of the tested B. bassiana strains for third instar white grub larvae varied, with only two strains showing high entomopathogenic activity. At 25 degrees C, mortality reached 73?or Bay.5-Gal and 74?or Bay.1-Lep at 55 d post-infection, but was lower, 27?nd 29?respectively, at 12 degrees C. These two strains produced significantly higher mortality in adult and whitefly nymphs, with 65-75?ortality 6 d post-infection. Based on morphological characters, including production of ellipsoidal conidia, and molecular characters (ITS, partial 18S (SSU rDNA) and EF1-alpha sequences), the isolates were identified as Beauveria bassiana belonging to Clade E from Asia. Our results add to data on the diversity of ecosystems inhabited by B. bassiana and provide a local resource for pest control efforts. |
URL (dc.rights) | https://www.sciencedirect.com/science/article/abs/pii/S0022201118303045?via=ihub |
DOI (dc.identifier.doi) | 10.1016/j.jip.2019.107243 |
Факультет / Институт (dc.identifier.fakulte) | Ziraat Fakültesi |
Бөлүмү (dc.identifier.bolum) | Bitki Koruma Bölümü |
Мекемедеги автор(лор) (dc.contributor.author) | Tinatin DOOLOTKELDİEVA |
Мекемедеги автор(лор) (dc.contributor.author) | Saykal BOBUŞOVA |
Мекемедеги автор(лор) (dc.contributor.author) | Sezim COLDOŞBEKOVA |
Каттоо № (dc.identifier.kayitno) | BL1C7A51D8 |
Каттоо киргизүү датасы (dc.date.available) | 2019-11-11 |
Эскертме (Жарыяланган жылы) (dc.identifier.notyayinyili) | October 2019 |
Wos No (dc.identifier.wos) | WOS:000492799800002 |
Предметтик рубрикатор (dc.subject) | beauveria bassiana isolates |
Предметтик рубрикатор (dc.subject) | entomopathogenic activity |
Предметтик рубрикатор (dc.subject) | biological control of soil and ground pests |