Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bacteria among isolates. The assignment to this taxonomic species has been corroborated by phylogenetic reconstruction using multilocus sequence analysis, and a short-sequence repeat (SSR) marker has been employed to estimate genetic diversity across the isolates. CRISPR analysis has revealed both a previously unreported CRISPR-2 array pattern and a close relationship of Kyrgyz E. amylovora isolates to strains present in Europe and the Middle East. This study presents the first consistent molecular taxonomic characterization of E. amylovora bacteria from Kyrgyzstan.
Басылманын аты (dc.title) | Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan |
Автор/лор (dc.contributor.yazarlar) | Tinatin Doolotkeldieva, Saykal Bobusheva, Christina Schuster, Maxabat Konurbaeva, Andreas Leclerque |
Басылманын түрү (dc.type) | Makale |
Тили (dc.language) | İngilizce |
Жарыяланган жылы (dc.date.issued) | 2019 |
Улуттук/Эл аралык (dc.identifier.ulusaluluslararasi) | Uluslararası |
Булагы (dc.relation.journal) | European Journal of Plant Pathology |
Саны (dc.identifier.issue) | 2 |
Том/№ (dc.identifier.volume) | 155 |
Барагы (dc.identifier.startpage) | 677-686 |
ISSN/ISBN (dc.identifier.issn) | ISSN: 0929-1873; Online ISSN: 1573-8469 |
Басмаканасы (dc.publisher) | Springer |
Маалымат базалар (dc.contributor.veritaban) | Web of Science Core Collection |
Маалымат базалар (dc.contributor.veritaban) | Springer |
Маалымат базалар (dc.contributor.veritaban) | Scopus |
Индекс түрү (dc.identifier.index) | SCI Expanded |
Индекс түрү (dc.identifier.index) | Scopus |
Импакт-фактору (dc.identifier.etkifaktoru) | 1,744 / 2018-WOS / 5 Year: 1,754 |
Қысқаша (dc.description.abstract) | Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bacteria among isolates. The assignment to this taxonomic species has been corroborated by phylogenetic reconstruction using multilocus sequence analysis, and a short-sequence repeat (SSR) marker has been employed to estimate genetic diversity across the isolates. CRISPR analysis has revealed both a previously unreported CRISPR-2 array pattern and a close relationship of Kyrgyz E. amylovora isolates to strains present in Europe and the Middle East. This study presents the first consistent molecular taxonomic characterization of E. amylovora bacteria from Kyrgyzstan. |
URL (dc.rights) | https://link.springer.com/article/10.1007/s10658-019-01790-3 |
DOI (dc.identifier.doi) | 10.1007/s10658-019-01790-3 |
Факультет / Институт (dc.identifier.fakulte) | Ziraat Fakültesi |
Бөлүмү (dc.identifier.bolum) | Bitki Koruma Bölümü |
Мекемедеги автор(лор) (dc.contributor.author) | Tinatin DOOLOTKELDİEVA |
Мекемедеги автор(лор) (dc.contributor.author) | Saykal BOBUŞOVA |
Мекемедеги автор(лор) (dc.contributor.author) | Mahabat KONURBAYEVA |
Каттоо № (dc.identifier.kayitno) | BLB5C86959 |
Каттоо киргизүү датасы (dc.date.available) | 2019-10-31 |
Эскертме (Жарыяланган жылы) (dc.identifier.notyayinyili) | October 2019 |
Wos No (dc.identifier.wos) | WOS:000490304400025 |
Предметтик рубрикатор (dc.subject) | fire blight |
Предметтик рубрикатор (dc.subject) | erwinia amylovora |
Предметтик рубрикатор (dc.subject) | Kyrgyzstan |
Предметтик рубрикатор (dc.subject) | diagnostic PCR |
Предметтик рубрикатор (dc.subject) | multilocus sequence analysis (MLSA) |
Предметтик рубрикатор (dc.subject) | short-sequence repeat (SSR) |
Предметтик рубрикатор (dc.subject) | CRISPR arrays |