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Researcher : İbrahim İlker ÖZYİĞİ ... ✕
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Prof. Dr. İbrahim İlker ÖZYİĞİTFen Fakültesi
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Construction, characteristics and high throughput molecular screening methodologies in some special breeding populations: a horticultural perspective

Hasan CAN | İbrahim İlker ÖZYİĞİT | Mustafa PAKSOY

Advanced marker technologies are widely used for evaluation of genetic diversity in cultivated crops, wild ancestors, landraces or any special plant genotypes. Developing agricultural cultivars requires the following steps: (i) determining desired characteristics to be improved, (ii) screening genetic resources to help find a superior cultivar, (iii) intercrossing selected individuals, (iv) generating genetically hybrid populations and screening them for agro-morphological or molecular traits, (v) evaluating the superior cultivar candidates, (vi) testing field performance at different location ...More

Article 2019 Ziraat Fakültesi Journal of Genetics98 ( 3 ) 61 Views
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Cotton Biotechnology: An Efficient Gene Transfer Protocol via Agrobacterium tumefaciens for a Greater Transgenic Recovery

İbrahim İlker ÖZYİĞİT | Yılmaz KAYA

Due to its economic worth, cotton (Gossypium hirsutum L.) is grown in almost 70 countries and provides income for more than 250 million people. Therefore, producing cotton with having some desired characteristics that includes extended biotic and abiotic stress tolerance, improved fiber quality, promoted nutritional content and increased yield is the main objective for cotton biotechnology. To achieve this goal, many tissue culture and gene transfer techniques are being developed and used throughout the years. As applications for the gene transfer, the Agrobacterium-mediated, particle bombardm ...More

Review 2022 Fen Fakültesi Journal of Natural Fibers19 ( 15 ) 115 Views
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Cotton Agriculture in Turkey and Worldwide Economic Impacts of Turkish Cotton

İbrahim İlker ÖZYİĞİT

Cotton is a product that provides wealth for humanity with its widespread and compulsory usage, and alongside creating employment opportunities in the producer countries. Due to the availability of suitable land for agriculture, seven countries including Turkey produce close to 80% of cotton worldwide. The average cotton yield in Turkey is above the world average, placing the country close to first place among the major producing countries. On the other hand, Turkey is one of the main countries engaged in organic cotton production by using only non-transgenic seeds and therefore, its cotton as ...More

Review 2022 Fen Fakültesi Journal of Natural Fibers19 ( 15 ) 147 Views
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An investigation on environmental pollution due to essential heavy metals: a prediction model through multilayer perceptrons

İbrahim İlker ÖZYİĞİT

This research is to predict heavy metal levels in plants, particularly in Robinia pseudoacacia L., and soils using an effective artificial intelligence approach with some ecological parameters, thereby significantly eliminating common defects such as high cost and seriously tedious and time-consuming laboratory procedures. In this respect, the artificial neural network (ANN) is employed to estimate the concentrations of essential heavy metals such as Fe, Mn and Ni, depending on the Cu and Zn concentrations of plant and soil samples collected from five different locations. The derived relative ...More

Article 2022 Fen Fakültesi International Journal of Phytoremediation25 ( 1 ) 104 Views
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Agrobacterium- mediated transformation of Turkish upland rice (Oryza sativa L.) for Dalapon herbicide tolerance

Yılmaz KAYA | İbrahim İlker ÖZYİĞİT | Ali ASLAN

Agrobacterium-mediated transformation of upland rice is established in few numbers of cultivars due to the high cultivar-specificity of regeneration from transformed explants. Further, dehalogenase E (dehE) gene had been characterized in Pseudomonas putida and it produces an enzyme that degrades dalapon. This study aimed to transform Turkish upland rice with the dehE herbicide resistant gene and addresses the challenges of transgenic rice recovery by identifying explant and transformation method. Constructed vector pCAMdehE carrying dehE gene was transferred into the rice shoot apex by Agrobac ...More

Article 2020 Fen Fakültesi Indian Journal of Biotechnology (IJBT)19 ( 4 ) 134 Views
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Heavy Metal Levels and Mineral Nutrient Status of Natural Walnut (Juglans regia L.) Populations in Kyrgyzstan: Nutritional Values of Kernels

Bakıt BORKOYEV | Kalıypa SALİYEVA | Ali Osman SOLAK | İbrahim İlker ÖZYİĞİT

In this study, mineral nutrient and heavy metal (Al, Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn) contents of the walnut kernels and their co-located soil samples collected from the four different zones of natural walnut forests (Sary-Chelek, Arslanbap, and Kara-Alma in Jalal-Abad Region and Kara-Shoro in Osh Region) in Kyrgyzstan were investigated. The highest concentrations for all elements determined in the soil samples were observed in the Sary-Chelek zone whereas the Arslanbap zone was found to be having the lowest concentrations except Fe and Zn. The highest concentrations in the kerne ...More

Article 2019 Mühendislik Fakültesi Biological Trace Element Research189 ( 1 ) 142 Views
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EVALUATION OF ALUMINUM STRESS INDUCED ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES IN ROMAN NETTLE

İbrahim İlker ÖZYİĞİT

In this current work, Roman nettle (Urtica pilulifera L.), a traditional medicinal plant that is very common and widespread species throughout Asia, Europe, and Northern Africa, was used as a model plant to investigate changes in antimicrobial activity following the application of aluminum stress. U pilulifera seedlings were grown in growth-room conditions and 0, 100, and 200 M AlCl3 were applied to the plants together with Hoagland solution (20 ml) for two months. The antimicrobial activities were tested against nine strains of bacteria (Salmonella sp., Staphyllococcus aureus, Escherichia col ...More

Article 2019 Fen Fakültesi Fresenius Environmental Bulletin28 ( 2A ) 63 Views
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ECOPHYSIOLOGICAL PROPERTIES OF TURKISH ENDEMIC CENTAUREA CONSANGUINEA DC

İbrahim İlker ÖZYİĞİT

This physiological and ecological study was performed on Centaurea consanguinea DC. to acquire information about certain characteristics (bioclimatic, edaphic, topographic, biotic etc.) of its habitat and distribution in Turkey, determine the requirements for germination, explore the soil-plant relations and obtain data on certain aspects of its population biology. Plant and co-located soil samples were collected from Amasya and Havza District (Samsun) in Turkey using standard methods. Various analyzes were employed for determining of physical, chemical and biological properties of the plant s ...More

Article 2019 Fen Fakültesi Fresenius Environmental Bulletin28 ( 2A ) 48 Views
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DNA fingerprinting and assessment of some physiological changes in Al-induced Bryophyllum daigremontianum clones

İbrahim İlker ÖZYİĞİT

Aluminum (Al) is one of the most important stress factors that reduce plant productivity in acidic soils. Present work thereby analyzed Al-induced genomic alterations in Bryophyllum daigremontianum clones using RAPD and ISSR markers, and investigated responding changes in photosynthetic pigment (chlorophyll a, b, a/b, total chlorophyll and carotenoid) contents and total soluble protein amounts in plant leaves. The main reason for the use of bulbiferous spurs originated clone plants was to increase reliability and acceptability of RAPD and ISSR techniques in DNA fingerprinting. Raised 40 clone ...More

Article 2019 Fen Fakültesi Molecular Biology Reports46 ( 3 ) 65 Views
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COMPARATIVE ANALYSIS OF MATURE AND PREPROPROTEIN FORM OF THIONINS IN SOME PLANT SPECIES; BIOINFORMATICS APPROACHES

İbrahim İlker ÖZYİĞİT

WOS:000471836900075

Article 2019 Fen Fakültesi Fresenius Environmental Bulletin28 ( 5 ) 86 Views
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Comparative analyses of phytochelatin synthase (PCS) genes in higher plants

İbrahim İlker ÖZYİĞİT

Plants employ various defence strategies to ameliorate the effects of heavy metal exposures, leading to re-establishment of metal homeostasis. One of the strategies includes the biosynthesis of main heavy metal detoxifying peptides phytochelatins (PCs) by phytochelatin synthase (PCS). In the present study, 14 PCS homologues were identified in the genomes of 10 selected plants. The size of these PCSs was 452-545 amino acid residues, with characteristic phytochelatin and phytochelatin_C domains. The N-terminal site of the proteins is highly conserved, whereas the C-terminal site is less conserve ...More

Article 2019 Fen Fakültesi Biotechnology and Biotechnological Equipment33 ( 1 ) 62 Views
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Abiotic stress-induced regulation of antioxidant genes in different Arabidopsis ecotypes: microarray data evaluation

İbrahim İlker ÖZYİĞİT

Although stresses induce generation of reactive oxygen species (ROS), which are highly reactive and toxic, and cause severe damage to cellular components; plants have very efficient enzymatic ROS-scavenging mechanisms. Despite the substantial knowledge produced about these enzymes, we still have limited knowledge regarding their expression patterns in relation to the stress type, duration and strength. Thus, taking advantage of microarray data, this work evaluated the abiotic stresses (salt, cold, heat and light) induced regulation of six antioxidant enzymes, superoxide dismutase (SOD), catala ...More

Article 2019 Fen Fakültesi Biotechnology and Biotechnological Equipment33 ( 1 ) 100 Views
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