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The study was carried out to determine the effect of 2 ecological regions and lactation period on chemical (P < 0.01) and amino acid (P < 0.01) composition and nitrogen fraction (P < 0.01) of mares grazing on pastures at 1700 m (vegetation is dominated by shrubs and sub-shrubs) and 2200 m (vegetation is dominated by grasses, forbs, sub-shrubs) above sea level. The animals were kept under extensive pasture conditions and received no additional feed supplements. Milk samples were collected monthly from May to July and from May to August from mares grazing at 1700 m (Mountains) and 2200 m (Highla ...More