Ulaş ACARÖZ | Fatih Ramazan İSTANBULLUGİL | Damla ARSLAN ACARÖZ
The discovery of antibiotics saved many lives. Infections were not as deadly a problem for clinicians as they once were. However, due to inappropriate and excessive use of antibiotics, antibiotic resistance has increased dramatically worldwide. Infectious diseases are becoming more challenging to control, and they cause increased morbidity and mortality. Also, a significant risk to human health is posed by infections associated with biofilms. To combat these drug-resistant microorganisms, several novel and alternative strategies have been identified. Bee products such as honey, bee pollen, pro ...Daha fazlası
Bu çalışma, Merinos ırkı koyunlarda gebeliğin perifer kan lenfositlerinin alfa naftil asetat esteraz (ANAE) ve asit fosfataz (ACP-az) aktiviteleri ile perifer kan lenfosit oranları üzerindeki etkilerinin belirlenmesi amacıyla yapıldı. Hayvanlardan her grupta 20’şer adet olacak şekilde gebe olmayan-kontrol, bir aylık, iki aylık, üç aylık, dört aylık ve beş aylık gebeler olmak üzere toplam 6 dönemde perifer kanlar alındı. Histolojik incelemeler sonucunda en düşük ANAE pozitif-lenfosit oranı (%63.5) bir aylık gebe koyunlarda tespit edilirken aynı dönemde null lenfosit sayısı da en yüksek seviyede ...Daha fazlası
Bu çalışmanın amacı, Kangal Akkaraman Koyunu ıslah projesi kapsamında, populasyon seviyesinde genetik çeşitliliğin tespiti ve DNA seviyesinde ebeveyn tayininin olasılığının araştırılmasıdır. Pilot bir çalışma olarak, kapalı ve kan yakınlığı değerlerinin yüksek olduğu tahmin edilen iki Kangal Akkaraman Koyunu sürüsünde damızlık olarak kullanılan toplam 13 koçtan kan örnekleri alınmıştır. Toplam 20 mikrosatellit lokusu kullanılarak polimeraz zincir reaksiyonu (PZR) ile spesifik genom bölgeleri çoğaltılmıştır. PZR ürünleri kapiller elekroforez ile ayrıştırılarak allel ve genotipleri belirlenmişti ...Daha fazlası
Encephalitic listeriosis is the most significant purulent encephalitis in ruminants and is a very common endemic problem in sheep, cattle, and goats. In this study, it was aimed to compare the presence of Listeria (L.) monocytogenes revealed by immunohistochemical (IHC) and Real-Time PCR methods with histopathological findings obtained from the archive materials. The study material consisted of pons and medulla oblongata paraffin tissue of 100 ruminants (9 cattle, 4 calves, 44 sheep, 38 lambs, and 5 goats). Positivity was obtained by the IHC method in 46 (46) and by the Real-Time PCR method in ...Daha fazlası
Ayperi Aytmırza Kızı | Zarima CUMAKANOVA | Ayday CUNUŞOVA
Canine hepatozoonosis is a tick-borne protozoan disease and widespread in Europe, Africa, Asia and America. There is not any available data about the presence of Hepatozoon infections in dogs in Kyrgyzstan. In the study we aimed that investigate the presence of Hepatozoon canis and the prevalance of Hepatozoon infections in dogs from Kyrgyzstan using polymerase chain reaction (PCR) and sequence analysis. To determine the prevelance of hepatozoonosis in dogs, a total of 170 blood samples were applied to PCR to amplify a fragment of 666 bp found in 185 SSU rRNA gene of Hepatozoon spp. The PCR re ...Daha fazlası
İhsan KISADERE | Nariste KADIRALİYEVA | Ertan ORUC
The aim of this study was to determine the physiological, hematological parameters and ANAE positivity of donkeys in Kyrgyzstan. Animals (n=24) were clinically examined and blood samples were taken. The average pulsation and respiration rates of male and female donkeys were measured as 52.25±9.27; 49.16±4.80 beats/minute and 18.41±4.21; 18.58±3.30 breaths/minute, respectively. HGB, MCV, MCH and MCHC values did not different in the groups. RBC and WBC values were higher in females. Mean ANAE-positive PBL ratio of donkeys was found as 42.90±1.18%. Consequently, some physiological, hematological ...Daha fazlası
In this study, it was aimed to investigate polymorphisms in seven genes (GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2) related to reproductive traits in dairy heifers. Frequency distributions of the genotypes between fertile and repeat breeder heifers groups were investigated. Allele effects on fertility were also analyzed. Blood samples were taken from a total of 160 Holstein-Friesian heifers and they were divided into two groups according to their artificial insemination numbers (AI). The heifers becoming pregnant after the first AI were used as the fertile heifers (FH, n= 80) and the heifers w ...Daha fazlası
The aim of this study was to evaluate a simple and rapid DNA extraction method combined with a multiplex polymerase chain reaction (mPCR) for the identification of the major mastitis pathogens (Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli and Mycoplasma bovis) from milk samples. Of the 200 California Mastitis Test (CMT) positive milk samples, 45 (22.5%), 21 (10.5%) and 11 (5.5%) were detected as positive for the presence of S. aureus, S. agalactiae and E. coli by culture, respectively. In mPCR by DNA isolation method optimised here, S. aureus, S. agalactiae and E. coli wer ...Daha fazlası
Bu çalışma, gebeliğin farklı dönemlerindeki fare desidua bazalis dokusunda periyodik-asit Schiff (PAS)-pozitif granüllere sahip uterus doğal katil (uNK) hücrelerinin dağılımının belirlenmesi amacıyla yapıldı. Bu amaçla, 12-14 haftalık fareler, gebe olmayan-kontrol grubu ile gebeliğin birinci, ikinci ve üçüncü haftalarının ortasına karşılık gelecek şekilde erken, orta ve geç gebelik dönemi (sırasıyla gebeliğin 3, 10. ve 17. günleri) olmak üzere 4 gruba ayrıldılar (n= 6). Plasentasyonun ilerlemesiyle birlikte metriyal bölge (MLAp; mesometrial lymphoid aggregate of pregnancy), desidua bazalis, ba ...Daha fazlası
This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma phagocytophilum in small ruminants from Bingol, Elazig, Malatya and Mus provinces. A total of 422 (291 sheep and 131 goats) blood samples were collected from apparently healthy animals. To determine of A. ovis and A. phagocytophilum in small ruminants, species-specific PCRs were set up using 60 kDA chaperonin gene (cpn60, also known as hsp60 or groEL) and 16S SSU rRNA gene primer sets, respectively. A total of 301 (71.32%) animals were found infected with A. ovis and/or A. phagocytophilum . The p ...Daha fazlası
Bu çalışma, farelerde gebeliğin T-lenfosit, null lenfosit ve asit fosfataz (ACP-az) pozitif lenfositlerin perifer kandaki oranı ve endometriyumun desidua bazalis bölgesindeki dağılımı üzerindeki etkilerinin belirlenmesi amacıyla yapıldı. Bu amaçla, 12-14 haftalık fareler, gebe olmayan-kontrol grubu ile gebeliğin birinci, ikinci ve üçüncü haftalarının ortasına karşılık gelecek şekilde erken, orta ve geç gebelik dönemi (sırasıyla gebeliğin 3., 10. ve 17. günleri) olmak üzere 4 gruba ayrıldılar (n = 6). En düşük T-lenfosit oranı perifer kanda (%43.83) ve desidua bazalis dokusunda (10.83 adet/0.1 ...Daha fazlası
Mare's milk and koumiss are two very important basic foodstuffs in central Asia.This study aimed to research the AFM(1) contamination in mare's milk and koumiss samples collected from local producers in the highlands in Naryn, Issyk-Kul, Bishkek and Talas regions of Kyrgyz Republic. For this purpose, 75 raw mare's milk and 75 koumiss samples collected in May, June and July 2017 were analyzed using the ELISA method. ARM was detected in 13.3% of the 75 raw mare's milk samples with levels ranging between 6.48 and 25.45 ng/L. Approximately 53.3% of the koumiss samples were found to contain AFM(1), ...Daha fazlası