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Researcher : Saykal BOBUŞOVA ✕
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Dr. Saykal BOBUŞOVAZiraat Fakültesi
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Identification and Prevalence of Ralstonia Solanacearum From Potato Fields of Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed duri ...More

Article 2014 Ziraat Fakültesi MANAS Journal of Agriculture, Veterinary and Life Science (MJAVL)4 ( 1 ) 65 Views
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Endophytic fungi diversity of wild terrestrial plants in Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

A total of 255 species of wild medicinal plants were analyzed in Northern and Northeastern Kyrgyzstan; from 150 plant species, 278 endophytic fungal isolates were identified. Endophytic fungi were frequently recorded on members of large plant families: Asteraceae, Ranunculaceae, Rutaceae, Fabaceae,Lamiaceae, Boraginaceae, Moraceae, Iridaceae, Euphorbiaceae and Brassicaceae. In the foothill ecosystems, rich biodiversity and numerous species of endophytic fungi genera - Fusarium, Acremonium, Cladosporium, Penicillium, Curvularia, Monilia, Rhizoctonia, Papularia, Botrytis and Stemphylium - were r ...More

Article 2014 Ziraat Fakültesi Global Advanced Research Journal of Microbiology3 ( 10 ) 65 Views
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Микробные сообщества высокогорных и низкотемпературных почв долины Сон-Куль Кыргызстана

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

С помощью классического метода микробиологии и анализа последовательностей 16SrRNA впервые было изучено количественное соотношение и биоразнообразие микробных комплексов низкотемпературных почв долины Сон-Куль. Структура видового состава бактерий отличалась в почвах под различным покровам растительности по мере возрастания высоты местности. Анализ последовательностей 16SrRNA показал, что в летний период в почвах высокогорной долины Сон-Куль доминирующими филогенетическими группами были Actinobacteria (55,0%), Gammaproteobacteria ( 33,0%) и Firmicutes phyla (22,0%). Видовой состав обнаруженных ...More

Article 2012 Ziraat Fakültesi Фундаментальные исследования ( 9; Часть 2 ) 79 Views
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Screening of Wild-Type Fungal Isolates for Cellulolytic Activity

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Background: In this study, wild-type fungal isolates, producing highly effective cellulolytic enzymes were selected for bioconversion of residues and waste from agriculture and rational utilization of energy resources for food production. Methods: We screened wild-type fungal isolates of Aspergillus, Penicillium, and Trichoderma with an enhanced ability to produce extracellular cellulase. We carried out solid-state fermentation on a medium of agricultural waste products, including wheat bran, beet peels, and cotton oil cake, as well as additional sources of nitrogen and mineral elements. Enzym ...More

Article 2011 Ziraat Fakültesi Microbiology Insights ( 4 ) 74 Views
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Biological Activity of Ag and Cu Monometallic Nanoparticles and Ag-Cu Bimetallic Nanocomposites against Plant Pathogens and Seeds

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

The antibacterial activities of silver (Ag) and copper (Cu) monometallic and bimetallic nanoparticles (MNPs and BNPs) against the most economically important and common plant bacterial diseases in Kyrgyzstan were tested, as well as their phytotoxic effects on wheat and corn seeds. To achieve this, a range of Ag and Cu MNPs and BNPs were synthesized using different methods and were tested at different concentrations to compare their biological effects. The antibacterial activities of Ag and Cu MNPs and Ag-Cu BNPs against the plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and ...More

Article 2022 Ziraat Fakültesi Journal of Nanomaterials2022 95 Views
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Microbial communities of vegetable seeds and biocontrol microbes for seed treatment

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Thirty-one species of vegetable seeds from Kyrgyzstan's State Register were analysed for mycological and bacterial composition using various methods, from simple visual to molecular. The modified roll-towel method simultaneously assesses the degree of contamination and seed germination. Screening in agar medium allows analysis of species on the outer and inner surfaces of vegetable seeds that are not detected using the roll-towel method. As a result of the phytopathological examination, 15 fungal and three bacterial species were found. Direct DNA extraction and amplification of the universal 1 ...More

Article 2022 Ziraat Fakültesi Seed Science and Technology50 ( 1-3 ) 34 Views
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LOW TEMPERATURE AND VEGETATION EFFECTS ON THE SOIL BACTERIAL COMMUNITIES STRUCTURE IN HIGH MOUNTAINOUS AND COLD BIOTOPES IN KYRGYZSTAN

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

It is well known that soil microorganisms play essential roles in the biogeochemical cycling of biogenic elements and soil-forming processes. However, little is known about the effect of the vegetation type on the bacterial community structures in soils from cold regions. For these reasons, we have analysed the bacterial communities of eight biotopes covered with different plants and two biotopes without vegetation in the Son-Kull Valley as the coldest corner in Kyrgyzstan. Using the culture-depended and culture-independent (16S rRNA gene sequencing) methods, we found 4 phylum (Actinobacteria- ...More

Article 2022 Ziraat Fakültesi Applied Ecology and Environmental Research20 ( 5 ) 43 Views
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Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bac ...More

Article 2019 Ziraat Fakültesi European Journal of Plant Pathology155 ( 2 ) 55 Views
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Characterization of Beauveria bassiana isolates from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Sezim COLDOŞBEKOVA

We report on the enzootic foci of the insect pathogenic fungus, Beauveria bassiana, found in high meadows in the middle mountain steppes of Kyrgyzstan, at elevations from 1000 m to 2200 m. The growth characteristics of various B. bassiana isolates on different media and as a function of temperature were studied. In addition, the ability of the fungal isolates to produce enzymes with amylase, protease and lipase activities was investigated. Dense biomass production on inexpensive solid media (oatmeal and bean oil meal) produced conidia used for insect bioassays targeting white grub larvae (Phyl ...More

Article 2019 Ziraat Fakültesi Journal of Invertebrate Pathology167 36 Views
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Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

In Kyrgyzstan, many former storehouses and dump sites for obsolete pesticides exist. In 2009/2010, an inventory and assessment of these sites including risks of environmental hazard has been conducted by FAO and the World Bank. Monitoring revealed high concentration of pesticides listed as persistent organic pollutants (POPs). The purpose of this research was to study the microbial structural complexes of the pesticide-contaminated soils in these dumping zones, and to search for and select microorganism’s destructors with cytochrome P450 genes for pesticide degradation. Culture-dependent and c ...More

Article 2018 Ziraat Fakültesi Environmental Science and Pollution Research25 ( 32 ) 62 Views
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Copper Nanoparticles: Synthesis and Biological Activity

Emil ÖMÜRZAK | Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

By means of XRD and FESEM analysis, it is established that copper nanoparticles with sizes less than 10 nm are formed during the chemical reduction, which form aggregates mainly with spherical shape. Presence of gelatin during the chemical reduction of copper induced formation of smaller size distribution nanoparticles than that of nanoparticles synthesized without gelatin and it can be related to formation of protective layer. Synthesized Cu nano-powders have sufficiently high activity against the Erwinia amylovora bacterium, and the bacterial growth inhibition depends on the Cu nanoparticles ...More

Proceedings Paper 2018 Mühendislik Fakültesi 2017 International Conference on Aerospace Technology, Communications and Energy Systems (ATCES 2017)302 ( UNSP 012075 ) 187 Views
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Scab Disease Caused by Venturia inaequalis on Apple Trees in Kyrgyzstan and Biological Agents to Control This Disease

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Among the most economically important fruit crops in South and North Kyrgyzstan affected by scab disease are apples (Malus domestica). Natural isolates of Venturia inaequalis were isolated from diseased parts of apple trees, selected from different regions, through sedimentation of air microflora on the crowns of fruit trees. Two biocontrol agents as Trichoderma viride and Streptomyces sр. were tested for apple scab control in vitro and field conditions. Two applications of Trichoderma viride within 35 days completely stopped the scab disease in seedling leaves. Within 40 days, the seedlings w ...More

Article 2017 Ziraat Fakültesi Advances in Microbiology7 ( 6 ) 60 Views
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