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Researcher : Saykal BOBUŞOVA ✕
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Dr. Saykal BOBUŞOVAZiraat Fakültesi
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Characterization of Pseudomonas syringae PV. syringae from diseased stone fruits in Kyrgyzstan and testing of biological agents against pathogen

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan. The conventional phenotypic (LOPAT, API tests) and PCR-assisted isolation were used for the identificationof Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach (Prunus persica), cherry (Prunus subgen), apricot (Prunus armeniaca), and plum (Prunus salicina) samples taken from the Chy, Is ...More

Article 2020 Ziraat Fakültesi International Journal of Phytopathology9 ( 2 ) 16 Views
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An app for apples: Citizen-led mapping of fire blight in Central Asia

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Fire blight, caused by the bacterial pathogen Erwinia amylovora, is a severe bacterial disease of apple and pear that can quickly destroy whole plants. In the last decade, it was also detected in Central Asia, where wild pomaceous fruit plants represent the dominant species in mid-altitude forests and constitute a critical foundation for the entire ecosystem. Efficiently informing farmers, forestry services and private persons about the instances and dangers of fire blight, the correct way to recognize the symptoms, and the methods of disease control is thus of paramount importance in a vast a ...More

Article 2023 Ziraat Fakültesi Journal of Plant Pathology ( Published online: 30 May 2023 ) 36 Views
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Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS se ...More

Article 2023 Ziraat Fakültesi Journal of Fungi9 ( 10 ) 26 Views
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Identification of Microbial Populations Present in Agricultural and Nonarable Soils in the Talas Valley, Northern Kyrgyzstan, in Autumn

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Soil bacterial and fungal communities were investigated in relation to soil type and farm management practices after vegetation harvesting in autumn. Soils from fields cultivated with Phaseolus vulgaris (bean) and Pyrus comminus (pear) and nonarable, natural areas were studied. Microbial diversity was analysed using cultivation-dependent methods (isolation of pure cultures) and cultivation-independent methods (direct extraction of DNA from soil, followed by PCR amplification of the 16S rRNA and 18S rRNA genes). The use of cultivation-dependent methods revealed that there were no differences in ...More

Article 2023 Ziraat Fakültesi Applied and Environmental Soil Science2023 17 Views
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In vitro and in vivo screening of bacterial species from contaminated soil for heavy metal biotransformation activity

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

Heavy metals (HMs) are widely used in various industries. High concentrations of HMs can be severely toxic to plants, animals and humans. Microorganism-based bioremediation has shown significant potential in degrading and detoxifying specific HM contaminants. In this study, we cultivated a range of bacterial strains in liquid and solid nutrient medium containing different concentrations of different HMs to select and analyze bacteria capable of transforming HMs. The bacterial strains most resistant to selected HMs and exhibiting the ability to remove HMs from contaminated soils were identified ...More

Article 2024 Ziraat Fakültesi Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes ( Published online: 27 Apr 2024 ) 0 Views
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In situ microbial bioremediation of obsolete pesticides at their disposal sites

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

Soil pollution by pesticides is a global problem in many countries, including Kyrgyzstan. Currently, 50 storage facilities for obsolete banned pesticides exist, severely threatening the surrounding populations and environment. In this study, in situ bioremediation technology was used to remove pesticide contamination from soil around the storage facilities. Three conditions were tested: application of fertile soil and bioproduct, application of fertile soil and no bioproduct (Control 1), and application of fertile soil and no bioproduct (Control 2). An aerobic bioremediation approach was used: ...More

Article 2024 Ziraat Fakültesi Bioremediation Journal ( Published online: 25 Apr 2024 ) 0 Views
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Biological Control of Erwinia carotovora ssp. carotovora by Streptomyces Species

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Ten isolates of Erwinia carotovora ssp. carotovora (Ecc) were isolated from infected potato tubers of Picasso, Sante, and Nevskiy varieties collected from different regions in Kyrgyzstan. Isolates were identified as Erwinia carotovora ssp. carotovora (Ecc) by standard bacteriological techniques and pathogenicity tests on tubers and also by PCR analyses. Tests on the pathogenicity of E. carotovora ssp. carotovora (Ecc) strains to host plants by artificial inoculation have shown a high sensibility of the Picasso variety. As a result, five isolates were chosen, three isolates (EcPo1, EcPo2, and E ...More

Article 2016 Ziraat Fakültesi Advances in Microbiology6 ( 2 ) 81 Views
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Fire Blight Disease Caused by Erwinia amylovora on Rosaceae Plants in Kyrgyzstan and Biological Agents to Control This Disease

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Erwinia amylovora species were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical and pathogenicity tests, alongside PCR analyses, were conducted to identify the local isolates of Erwinia amylovora. The alternative antagonistic microorganisms which combat bacterium E. amylovora were tested within in vitro and in vivo conditions. The results revealed the ability of Streptomyces antagonistic bacteria to decrease fire blight severity on pear and apple trees during ...More

Article 2016 Ziraat Fakültesi Advances in Microbiology6 ( 11 ) 133 Views
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Identification of Ralstonia Solanacearum in Kyrgyzstan's Potato Fields and the Possibility of Using Biocontrol Agents Against this Pathogen

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

In this study, we have used well-known, efficient methods and bioassay for systematic screening of R. solanacearum for identification of its phenotype and biochemical profile, as well as for pathogenicity and virulence. As a result, an aggressive race — Biovar 3 — was most isolated from the potato fields of the Issyk-Kul region, especially in fields where the Picasso variety was grown. The isolated indigenous strains of Streptomyces diastatochromogenesstrain sk-6 and Streptomyces bambergiensis strain k1-3 has the potential to be used as a biocontrol agent for the management of the bacterial wi ...More

Article 2016 Ziraat Fakültesi International Journal of Environmental & Agriculture Research (IJOEAR)2 ( 5 ) 56 Views
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Effects of Streptomyces Biofertilizer to Soil Fertility and Rhizosphere’s Functional Biodiversity of Agricultural Plants

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

In the present study, a biofertilizer on the basis of Streptomyces fumanus gn-2 was used for the treatment of wheat and soybean seeds (dose 104 spore/ml) before planting them in soil with low fertility in order to determine the effect of this biological agent on germination rate; the growth of seedlings, shoots, and the maturation phase of plants; the rhizosphere’s functional biodiversity; and the resistance of these plants to pathogens. Seeds were soaked in the suspension for a period of two or three hours. During the growing season of the crop, no additional fertilizing and spraying of a bio ...More

Article 2015 Ziraat Fakültesi Advances in Microbiology5 ( 7 ) 60 Views
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Bacterial Community Structure In Petroleum Contaminated And Uncontaminated Soils Of Mountain Ecosystem

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

We compared a microbial diversity in uncontaminated and contaminated with petroleum soils in mountain ecosystems in Kyrgyzstan. Culture-dependent and culture - independent methods were used to analyze microbial diversity. PCR primers that target conserved iron binding motifs in alkane monooxygenase (alkB) and cytochrome P450 alkane hydroxylase were used to analyze genes of alkane degrading bacteria in chronically contaminated and uncontaminated sites, using an enrichment culture with dodecane. Analysis of 16S rRNA showed that in contaminated soils BetaProteobacteria phylum populations were abu ...More

Article 2015 Ziraat Fakültesi MANAS Journal of Agriculture, Veterinary and Life Science (MJAVL)5 ( 1 ) 57 Views
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Impact of Biocontrol Agent Streptomyces fumanus on Bacterial Communities in the Rhizosphere of Wheat and Soybean in Newly Cultivated Soil

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

wheat and soybean seeds biocontrol agent rhizosphere microflora in vegetation phases impact of streptomyces fumanus on biodiversity of rhizosphere

Article 2015 Ziraat Fakültesi Global Advanced Research Journal of Agricultural Science4 ( 4 ) 146 Views
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