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Researcher : Tinatin DOOLOTKELDİE ... ✕
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Prof. Dr. Tinatin DOOLOTKELDİEVAZiraat Fakültesi
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LOW TEMPERATURE AND VEGETATION EFFECTS ON THE SOIL BACTERIAL COMMUNITIES STRUCTURE IN HIGH MOUNTAINOUS AND COLD BIOTOPES IN KYRGYZSTAN

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

It is well known that soil microorganisms play essential roles in the biogeochemical cycling of biogenic elements and soil-forming processes. However, little is known about the effect of the vegetation type on the bacterial community structures in soils from cold regions. For these reasons, we have analysed the bacterial communities of eight biotopes covered with different plants and two biotopes without vegetation in the Son-Kull Valley as the coldest corner in Kyrgyzstan. Using the culture-depended and culture-independent (16S rRNA gene sequencing) methods, we found 4 phylum (Actinobacteria- ...More

Article 2022 Ziraat Fakültesi Applied Ecology and Environmental Research20 ( 5 ) 43 Views
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Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bac ...More

Article 2019 Ziraat Fakültesi European Journal of Plant Pathology155 ( 2 ) 55 Views
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Characterization of Beauveria bassiana isolates from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Sezim COLDOŞBEKOVA

We report on the enzootic foci of the insect pathogenic fungus, Beauveria bassiana, found in high meadows in the middle mountain steppes of Kyrgyzstan, at elevations from 1000 m to 2200 m. The growth characteristics of various B. bassiana isolates on different media and as a function of temperature were studied. In addition, the ability of the fungal isolates to produce enzymes with amylase, protease and lipase activities was investigated. Dense biomass production on inexpensive solid media (oatmeal and bean oil meal) produced conidia used for insect bioassays targeting white grub larvae (Phyl ...More

Article 2019 Ziraat Fakültesi Journal of Invertebrate Pathology167 36 Views
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Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

In Kyrgyzstan, many former storehouses and dump sites for obsolete pesticides exist. In 2009/2010, an inventory and assessment of these sites including risks of environmental hazard has been conducted by FAO and the World Bank. Monitoring revealed high concentration of pesticides listed as persistent organic pollutants (POPs). The purpose of this research was to study the microbial structural complexes of the pesticide-contaminated soils in these dumping zones, and to search for and select microorganism’s destructors with cytochrome P450 genes for pesticide degradation. Culture-dependent and c ...More

Article 2018 Ziraat Fakültesi Environmental Science and Pollution Research25 ( 32 ) 62 Views
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Copper Nanoparticles: Synthesis and Biological Activity

Emil ÖMÜRZAK | Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

By means of XRD and FESEM analysis, it is established that copper nanoparticles with sizes less than 10 nm are formed during the chemical reduction, which form aggregates mainly with spherical shape. Presence of gelatin during the chemical reduction of copper induced formation of smaller size distribution nanoparticles than that of nanoparticles synthesized without gelatin and it can be related to formation of protective layer. Synthesized Cu nano-powders have sufficiently high activity against the Erwinia amylovora bacterium, and the bacterial growth inhibition depends on the Cu nanoparticles ...More

Proceedings Paper 2018 Mühendislik Fakültesi 2017 International Conference on Aerospace Technology, Communications and Energy Systems (ATCES 2017)302 ( UNSP 012075 ) 187 Views
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Scab Disease Caused by Venturia inaequalis on Apple Trees in Kyrgyzstan and Biological Agents to Control This Disease

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Among the most economically important fruit crops in South and North Kyrgyzstan affected by scab disease are apples (Malus domestica). Natural isolates of Venturia inaequalis were isolated from diseased parts of apple trees, selected from different regions, through sedimentation of air microflora on the crowns of fruit trees. Two biocontrol agents as Trichoderma viride and Streptomyces sр. were tested for apple scab control in vitro and field conditions. Two applications of Trichoderma viride within 35 days completely stopped the scab disease in seedling leaves. Within 40 days, the seedlings w ...More

Article 2017 Ziraat Fakültesi Advances in Microbiology7 ( 6 ) 60 Views
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The Improving Conditions for the Aerobic Bacteria Performing the Degradation of Obsolete Pesticides in Polluted Soils

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Currently, in the territory of Kyrgyzstan, 50 storage facilities of obsolete pesticides exist; they store about 5000 tons of these hazardous chemicals. The storage conditions have become unusable for a long time. They pose a serious threat to the people living there, livestock, and the environment. The main purpose of this research was the use of selected bacteria with cytochrome P450 genes for the bioremediation of polluted soils around the burial sites in model soil experiments. In the first trial of biodegradation experiments, one contaminated soil was used without any changes in chemical c ...More

Article 2021 Ziraat Fakültesi Air, Soil and Water Research14 150 Views
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Genetic characterization of Erwinia amylovora isolates detected in the wild walnut-fruit forest of South Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Erwinia amylovora, the causal agent of fire blight, was first reported in Kyrgyz orchards in 2008 and, within a few years, the disease has spread throughout most of the northern part of the country. As fire blight expanded and penetrated new areas, it also reached the unique ecosystem constituted by the Arslonbob wild walnut-fruit forests in South Kyrgyzstan. Here, we present the results of field surveys in the forests and in the nearby orchards and gardens of the Jalal-Abad region during the 2019 season. Further, we use genetic profiling of the CRISPR regions to compare the genotypes of the o ...More

Article 2021 Ziraat Fakültesi Journal of Plant Pathology103 ( SUPPL 1 ) 47 Views
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Isolation, Identification, and Characterization of the Nematophagous Fungus Arthrobotrys oligospora from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Ayperi MURATBEKOVA

Purpose Predatory fungi have been the subject of fundamental studies and their potential as biological control agents against parasitic plant nematodes has been assessed. The aim of the present study was to isolate and identify predatory fungi, performing in vitro and in vivo screening to select highly active strains to control parasitic nematodes. Methods Different nutrient media were used to isolate predatory fungi and determine their morphological and cultural properties. Identification was performed by classical and molecular biology methods. In vitro and in vivo screening was conducted to ...More

Article 2021 Ziraat Fakültesi Acta Parasitologica66 ( 4 ) 95 Views
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Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer

Tinatin DOOLOTKELDİEVA

Fire blight is the most devastating disease affecting pome fruit production globally. The pathogen is native to North America and was imported to western Europe in the 1950s, progressively spreading over the continent in the ensuing decades. Previous phylogenetic studies have revealed the extreme genetic homogeneity of the pathogen outside its center of origin, which makes epidemiological studies difficult. These are generally only possible using hypervariable regions of the genome such as those represented by CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), which are, howe ...More

Article 2021 Ziraat Fakültesi Phytopathology Research3 ( 1 ) 102 Views
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Synthesis of biologically active silver and copper nanocomposites

Tinatin DOOLOTKELDİEVA | Emil ÖMÜRZAK

In the joint chemical reduction of silver and copper ions in the presence of gelatin, regardless of the composition of the reaction medium, a nanocomposite was formed consisting of nanodispersed silver and copper particles. In this research, the dependence of phase composition and dispersity on the synthesis conditions, as well as antibacterial activity of synthesized silver and copper nanocomposites were studied. It has been established that the most highly dispersed silver and copper nanocomposites were formed in an alkaline medium. We have revealed that nanocomposites of Ag-Cu system which ...More

Article 2018 Ziraat Fakültesi Materials Research Express5 ( 8 ) 78 Views
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