Impact of Biocontrol Agent Streptomyces fumanus on Bacterial Communities in the Rhizosphere of Wheat and Soybean in Newly Cultivated Soil

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

wheat and soybean seeds biocontrol agent rhizosphere microflora in vegetation phases impact of streptomyces fumanus on biodiversity of rhizosphere

Identification and Prevalence of Ralstonia Solanacearum From Potato Fields of Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed duri ...More

Endophytic fungi diversity of wild terrestrial plants in Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

A total of 255 species of wild medicinal plants were analyzed in Northern and Northeastern Kyrgyzstan; from 150 plant species, 278 endophytic fungal isolates were identified. Endophytic fungi were frequently recorded on members of large plant families: Asteraceae, Ranunculaceae, Rutaceae, Fabaceae,Lamiaceae, Boraginaceae, Moraceae, Iridaceae, Euphorbiaceae and Brassicaceae. In the foothill ecosystems, rich biodiversity and numerous species of endophytic fungi genera - Fusarium, Acremonium, Cladosporium, Penicillium, Curvularia, Monilia, Rhizoctonia, Papularia, Botrytis and Stemphylium - were r ...More

Biological Control Agents for Crop Protection and Sustainability of Agro-ecosystems in Kyrgyztan

Tinatin DOOLOTKELDİEVA

The main agricultural activities in the Kyrgyz Republic are include the production of cereal grains, vegetables, potatoes and commercial crops such as cotton, tobacco, sugar beets and livestock. The sustainability of the agriculture sector was severely affected by the large scale mono-cropping approach of the Soviet era. Intensive agriculture has caused environmental pollution due to the excessive use of mineral fertilizers and pesticides. To combat this menace we have isolated and introduced non-chemical crop protection agents, the antifungal micro-organisms Streptomyces spp., producing antib ...More

Alkane monooxygenase diversity recovered from Central Asia sediments

Tinatin DOOLOTKELDİEVA

Previous studies in our lab and elsewhere showed that different microbial gene groups are obtained from geographically distant sites. Alkane monooxygenases activate terminal carbon-carbon bonds in the first step of alkane degradation with the addition of an oxygen to the compound, resulting in an alcohol product. The aim of this study was to test the hypothesis that unique microbial populations and alkane monooxygenase (alkB and Cytochrome P450) genes can be recovered from geographically distinct sites, with varying degrees of anthropogenic impact. Sediment was collected from six river and str ...More

Микробные сообщества высокогорных и низкотемпературных почв долины Сон-Куль Кыргызстана

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

С помощью классического метода микробиологии и анализа последовательностей 16SrRNA впервые было изучено количественное соотношение и биоразнообразие микробных комплексов низкотемпературных почв долины Сон-Куль. Структура видового состава бактерий отличалась в почвах под различным покровам растительности по мере возрастания высоты местности. Анализ последовательностей 16SrRNA показал, что в летний период в почвах высокогорной долины Сон-Куль доминирующими филогенетическими группами были Actinobacteria (55,0%), Gammaproteobacteria ( 33,0%) и Firmicutes phyla (22,0%). Видовой состав обнаруженных ...More

Screening of Wild-Type Fungal Isolates for Cellulolytic Activity

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Background: In this study, wild-type fungal isolates, producing highly effective cellulolytic enzymes were selected for bioconversion of residues and waste from agriculture and rational utilization of energy resources for food production. Methods: We screened wild-type fungal isolates of Aspergillus, Penicillium, and Trichoderma with an enhanced ability to produce extracellular cellulase. We carried out solid-state fermentation on a medium of agricultural waste products, including wheat bran, beet peels, and cotton oil cake, as well as additional sources of nitrogen and mineral elements. Enzym ...More

Microbiological Control of Flour-Manufacture: Dissemination of Mycotoxins Producing Fungi in Cereal Products

Tinatin DOOLOTKELDİEVA

Wheat grain and its products are widely consumed as fodder and basic daily food stuffs in Kyrgyzstan. Mycobiota is known to produce hazardous effects to a consumer since it produces mycotoxins. Henceforth, mycobiota starting from the field stage to flour, grain and flour samples were selected for mycological analysis from eight sites of flour manufacture: grain stored in storehouses before milling, mechanically cleaned grain, washed grain, grain dried and prepared for mill, roughly-milled flour, first grade flour and high grade flour. The samples were analyzed using classical mycological and i ...More

New Strains of Streptomyces as Producers of Biofungicides and Biological Stimulators for Protection of the Shoots and Seedlings of Tiang-Shang Spruce Fir (Picea Schrenkiana)

Tinatin DOOLOTKELDİEVA

During this work we detected 5 species of pathogenic fungi that damage seedlings and saplings of the Tiang-Shang spruce fir, which cause a perceptible damage to the coniferous nurseries of Northern Kyrgyzstan and require activities to protect the woods. The biological activity of Streptomyces metabolites for raising the resistance of seedlings to phytopathogenic fungi was tested in laboratory and field conditions during three seasons. In order to evaluate the biological activity of Streptomyces preparations as biological agents, we have soaked the seeds in the suspension of Streptomyces metabo ...More

Ecology and Prevalence of Actynomyces-Antagonists in Soils of Kyrgyzstan

Tinatin DOOLOTKELDİEVA

Biological Activity of Ag and Cu Monometallic Nanoparticles and Ag-Cu Bimetallic Nanocomposites against Plant Pathogens and Seeds

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

The antibacterial activities of silver (Ag) and copper (Cu) monometallic and bimetallic nanoparticles (MNPs and BNPs) against the most economically important and common plant bacterial diseases in Kyrgyzstan were tested, as well as their phytotoxic effects on wheat and corn seeds. To achieve this, a range of Ag and Cu MNPs and BNPs were synthesized using different methods and were tested at different concentrations to compare their biological effects. The antibacterial activities of Ag and Cu MNPs and Ag-Cu BNPs against the plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and ...More

Microbial communities of vegetable seeds and biocontrol microbes for seed treatment

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Thirty-one species of vegetable seeds from Kyrgyzstan's State Register were analysed for mycological and bacterial composition using various methods, from simple visual to molecular. The modified roll-towel method simultaneously assesses the degree of contamination and seed germination. Screening in agar medium allows analysis of species on the outer and inner surfaces of vegetable seeds that are not detected using the roll-towel method. As a result of the phytopathological examination, 15 fungal and three bacterial species were found. Direct DNA extraction and amplification of the universal 1 ...More