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Faculty / Institute : Veteriner Fakültesi ✕Researcher : Murat KARAHAN ✕
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Prof. Dr. Murat KARAHANVeteriner Fakültesi
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Development of A Multiplex PCR Method for Direct Detection of Common Mastitis Pathogens in Bovine Milk Samples = Mastitisli İnek Sütlerinde Önemli Patojenlerin Direkt Tespiti İçin Bir Multipleks PCR Yönteminin Geliştirilmesi

Murat KARAHAN

The aim of this study was to evaluate a simple and rapid DNA extraction method combined with a multiplex polymerase chain reaction (mPCR) for the identification of the major mastitis pathogens (Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli and Mycoplasma bovis) from milk samples. Of the 200 California Mastitis Test (CMT) positive milk samples, 45 (22.5%), 21 (10.5%) and 11 (5.5%) were detected as positive for the presence of S. aureus, S. agalactiae and E. coli by culture, respectively. In mPCR by DNA isolation method optimised here, S. aureus, S. agalactiae and E. coli wer ...More

Article 2017 Veteriner Fakültesi Kafkas Üniversitesi Veteriner Fakültesi Dergisi23 ( 6 ) 95 Views
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Determination of the Important Toxin Genes of Staphylococcus aureus Isolated from Meat Samples, Food Handlers and Food Processing Surfaces in Turkey

Murat KARAHAN

Staphylococcusaureus is a pathogenic microorganism causing food intoxications and producing many heat-stable toxins. In this study S. aureus prevalence, the presence of enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sej, sei) together with the toxic shock syndrome toxin gene (tsst) were investigated by mPCR. A total of 500 samples were obtained from different animal's meat (sheep, goat, cattle and chicken), hands of staff and surfaces in contact with food. Of 500 samples, 126 (25.2%) were contaminated with S. aureus. One or more staphylococcal enterotoxin (se) gene was determined in 87 ...More

Article 2020 Veteriner Fakültesi Israel Journal of Veterinary Medicine75 ( 2 ) 66 Views
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