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Prof. Dr. Orhan YAPICIVeteriner Fakültesi
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Canine coronavirus infection in dogs in Turkey: Virological and serological evidence

Orhan YAPICI

In the present study, virological and serological investigations were performed to determine the presence and prevalence of Canine corona virus (CCoV) infection in dog population in Turkey. Sera samples were analyzed for specific antibodies against CCoV by indirect enzyme linked immunosorbent assay (i-ELISA) while leukocyte samples were inoculated onto monolayers of Madin Darby Canine Kidney permanent cell culture. The cells were examined for viral antigen by direct immunofluorescence (IF) test after third passage. CCoV seropositivity was found in 46 (24.46%) of 188 dogs by indirect ELISA whil ...More

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Effect of different storage temperatures on the stability of bovine viral diarrhea virus RNA in blood samples

Orhan YAPICI

The present study was conducted to determine stability of Bovine viral diarrhea virus (BVDV) RNA stored at different temperatures. A total of 6 blood samples obtained from a private cattle farm, which were found to be antigen positive (Ag+) by direct ELISA method, were used in this study. BVDV Ag+ samples were stored separately at 4, 21 and 37oC for 1 month. The samples were analyzed on the 0, 1st, 2nd, 3rd and 4th weeks by ELISA for the presence of BVDV Ag and by RT-PCR for the presence of BVDV RNA. Stability of BVDV RNA was calculated using maximum concentration (Cmax) and area under the cur ...More

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A serological investigation of Blue Tongue Virus infection in sheep breeds in Karaman province = Karaman ilinde yetiştirilen koyunlarda Blue Tongue Virus enfeksiyonunun serolojik olarak araştırılması

Orhan YAPICI

Aim: The aim of this study was to describe the seroprevalence rate of Blue Tongue Virus in sheep flocks in Karaman. Materials and Methods: A total of 350 sheep blood serum samples were collected from 5 flocks (70 from each flocks) that were randomly selected. Samples were tested against to Blue Tongue Virus antibodies by a commercial competitive enzyme linked immunosorbent assay (cELISA). Results: Prevalence of antibodies to Blue Tongue Virus in flocks was 32.85%, 28.57%, 25.71%, 37.14%, 41.42%, respectively. Out of 350 serum samples, 116 (33.14%) were positive for Blue Tongue Virus specific a ...More

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First report on seroprevalence of bluetongue, border disease and peste des petits ruminants virus infections in sheep in Kyrgyzstan

Orhan YAPICI

In this study, the presence of antibodies against Bluetongue Virus (BTV), Border Disease Virus (BDV) and Peste des Petits Ruminants virus (PPRV), were evaluated for the first time in Kyrgyzstan. A total of 655 serum samples were collected from healthy sheep (Jaydara breed) from different regions of Kyrgyzstan (144 from Issik Gol region, 208 from Narin region, 189 from Talas region and 114 from Çuy region). Commercially available competitive ELISA kits were used to detect antibodies against tested samples. Seroprevalence was found to be 36.94%, 7.32% and 35.11% against BTV, BDV and PPRV infecti ...More

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Detection of Antibodies Against Blue Tongue Virus in Yaks (Bos Grunniens) in Issyk Kul, First Report

Orhan YAPICI

Bluetongue virus (BTV) caused infection is commonly determined in ruminants including sheep and cattle. However, there is a little information about the seroprevalence of BTV in yaks. The aim of this study was to describe the seroprevalence rate BTV in unvaccinated yaks in Issyk Kul, Kyrgyzstan. A total of 168 serum samples were collected from yaks between September to November 2012. Antibodies to BTV in sera were detected using a commercially available competitive enzyme-linked immunosorbent assay. 2.38% seroprevalence (n: 4) was determined in the samples as positive for BTV antibodies. This ...More

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The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs

Orhan YAPICI

Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from 111 dogs, which were admitted to the Internal Medicine Clinic of Selcuk University, Faculty of Veterinary Medicine, with clinical symptoms. Seventy-seven dogs were sampled from Isparta and Burdur dog shelters by random sampling, regardless of the clinical findings. Dogs showed a systemic disease, characterized by fever, ...More

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Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem

Orhan YAPICI

Aim: The aim of this study is to determine the presence of Bovine Viral Diarrhea Virus (BVDV) infection in a cattle herd with abortion problem in Konya. Materials and Methods: Totally 228 blood serum and 228 leukocytes taken from cattle selected according to criteria for infertile and abortion problems were examined for antigens and antibodies to BVDV by Enzyme Linked Immunosorbent Assay. Results: In this research, 41 (17.9%) sera were found seropositive and 4 (1.7%) leukocytes were BVDV antigen positive. Of these 4 BVDV antigen positive cattle, a number of 2 (0.8%) were detected seropositive ...More

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Sütçü sığırların kan ve süt serumlarında Bovine Viral Diarrhea Virus antikorlarının ELISA ile belirlenmesi

Orhan YAPICI

Amaç: Bu çalışma kan ve süt serumlarının Bovine Viral Diarrhea Virus (BVDV) antijen varlığı ve BVDV'ye karşı gelişen antikorların tespit edilmesi amacıyla yapıldı. Gereç ve Yöntem: Çalışmada toplam 202 adet sığır kan ve süt serum örnekleri kullanıldı. Çiftliklerden elde edilen serum örnekleri BVDV antijen varlığı yönünden direkt Enyzme Linked Immunosorbent Assay (ELISA) ile BVDV'ye karşı gelişen antikor varlığı yönünden ise indirekt ELISA ile incelendi. Bulgular: 167 adet (%82.6) kan serumu ve 160 adet (%79.2) süt serum örneği BVDV antikorları yönünden pozitif belirlendi. Araştırmada 148 adet ...More

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Köpeklerde Canine Parainfluenza Virus Tip 2'nin immunfloresan ile araştırılması

Orhan YAPICI

Amaç: Köpeklerde Canine Parainfluenza Virus Tip 2 (CPIV- 2)'nin varlığının immunfloresan (IF) ile araştırılması amaçlandı. Gereç ve Yöntem: Klinik olarak solunum sistemi belirtileri gösteren 61 adet köpekten nazal ve konjuktival svap örnekleri alındı. Svaplar (toplam 132 adet) Madin-Darby Canine Kidney (MDCK) devamlı hücre kültürlerinde 3 kez pasajlandı. Son pasajdan elde edilen hücre kültürü süpernatantları CPIV-2 antijen varlığı yönünden IF ile incelendi. Bulgular: Svap örnekleri MDCK hücre kültürlerine inokule edildikten sonra 3 kez pasajlandı, ancak herhangi bir CPE tespit edilmedi. Tüm ör ...More

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Sütçü İneklerin Kan ve Süt Serumlarında Bovine Leukosis Virus Enfeksiyonunun ELISA ile Araştırılması = Investigation of Bovine Leukosis Virus Infection in Blood and Milk Serum in Dairy Cattle by ELISA

Orhan YAPICI

Bu çalışma kan ve süt serumlarında Bovine Leukosis Virus (BLV)'a karşı gelişen antikorların tespit edilmesi ve kan serumu yerine süt serumu örneklerinin kullanılıp kullanılamayacağının belirlenmesi amacıyla yapıldı. Mart-Eylül 2010 tarihleri arasında Isparta'nın ilçelerindeki (Keçiborlu, Gönen, Şarkikaraağaç, Aksu, Yalvaç, Sütçüler, Atağbey, Eğirdir) özel işletmelerde bulunan klinik olarak sağlıklı görünümde BLV'ye karşı aşılanmamış 428 baş Holştayn ırkı (yaşları 2 ile 9 arasında) sağmal inekten serolojik incelemeler amacıyla kan ve süt örnekleri alındı. Aynı hayvanlardan elde edilen kan ve sü ...More

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Serologic investigation for West Nile Virus Infection in Commercial Domestic Chickens (Gallus gallus domesticus)

Orhan YAPICI

In this study, West Nile Virus antibody presence (WNV) in white leghorn chickens located in various commercial domestic chicken establishments in Konya was studied serologically. Blood sampling from 380 white leghorn chickens within the age range of 20-40 weeks was carried out. Blood serum samples were studied by West Nile Competition ELISA kit. All samples were found seronegative.

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