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Index Type : Diğer Hakemli Dergi ✕Researcher : Tinatin DOOLOTKELDİE ... ✕
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Prof. Dr. Tinatin DOOLOTKELDİEVAZiraat Fakültesi
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Biological Control of Erwinia carotovora ssp. carotovora by Streptomyces Species

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Ten isolates of Erwinia carotovora ssp. carotovora (Ecc) were isolated from infected potato tubers of Picasso, Sante, and Nevskiy varieties collected from different regions in Kyrgyzstan. Isolates were identified as Erwinia carotovora ssp. carotovora (Ecc) by standard bacteriological techniques and pathogenicity tests on tubers and also by PCR analyses. Tests on the pathogenicity of E. carotovora ssp. carotovora (Ecc) strains to host plants by artificial inoculation have shown a high sensibility of the Picasso variety. As a result, five isolates were chosen, three isolates (EcPo1, EcPo2, and E ...More

Article 2016 Ziraat Fakültesi Advances in Microbiology6 ( 2 ) 81 Views
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Fire Blight Disease Caused by Erwinia amylovora on Rosaceae Plants in Kyrgyzstan and Biological Agents to Control This Disease

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Erwinia amylovora species were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical and pathogenicity tests, alongside PCR analyses, were conducted to identify the local isolates of Erwinia amylovora. The alternative antagonistic microorganisms which combat bacterium E. amylovora were tested within in vitro and in vivo conditions. The results revealed the ability of Streptomyces antagonistic bacteria to decrease fire blight severity on pear and apple trees during ...More

Article 2016 Ziraat Fakültesi Advances in Microbiology6 ( 11 ) 133 Views
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Identification of Ralstonia Solanacearum in Kyrgyzstan's Potato Fields and the Possibility of Using Biocontrol Agents Against this Pathogen

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

In this study, we have used well-known, efficient methods and bioassay for systematic screening of R. solanacearum for identification of its phenotype and biochemical profile, as well as for pathogenicity and virulence. As a result, an aggressive race — Biovar 3 — was most isolated from the potato fields of the Issyk-Kul region, especially in fields where the Picasso variety was grown. The isolated indigenous strains of Streptomyces diastatochromogenesstrain sk-6 and Streptomyces bambergiensis strain k1-3 has the potential to be used as a biocontrol agent for the management of the bacterial wi ...More

Article 2016 Ziraat Fakültesi International Journal of Environmental & Agriculture Research (IJOEAR)2 ( 5 ) 56 Views
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Effects of Streptomyces Biofertilizer to Soil Fertility and Rhizosphere’s Functional Biodiversity of Agricultural Plants

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

In the present study, a biofertilizer on the basis of Streptomyces fumanus gn-2 was used for the treatment of wheat and soybean seeds (dose 104 spore/ml) before planting them in soil with low fertility in order to determine the effect of this biological agent on germination rate; the growth of seedlings, shoots, and the maturation phase of plants; the rhizosphere’s functional biodiversity; and the resistance of these plants to pathogens. Seeds were soaked in the suspension for a period of two or three hours. During the growing season of the crop, no additional fertilizing and spraying of a bio ...More

Article 2015 Ziraat Fakültesi Advances in Microbiology5 ( 7 ) 60 Views
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Bacterial Community Structure In Petroleum Contaminated And Uncontaminated Soils Of Mountain Ecosystem

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

We compared a microbial diversity in uncontaminated and contaminated with petroleum soils in mountain ecosystems in Kyrgyzstan. Culture-dependent and culture - independent methods were used to analyze microbial diversity. PCR primers that target conserved iron binding motifs in alkane monooxygenase (alkB) and cytochrome P450 alkane hydroxylase were used to analyze genes of alkane degrading bacteria in chronically contaminated and uncontaminated sites, using an enrichment culture with dodecane. Analysis of 16S rRNA showed that in contaminated soils BetaProteobacteria phylum populations were abu ...More

Article 2015 Ziraat Fakültesi MANAS Journal of Agriculture, Veterinary and Life Science (MJAVL)5 ( 1 ) 57 Views
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Impact of Biocontrol Agent Streptomyces fumanus on Bacterial Communities in the Rhizosphere of Wheat and Soybean in Newly Cultivated Soil

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

wheat and soybean seeds biocontrol agent rhizosphere microflora in vegetation phases impact of streptomyces fumanus on biodiversity of rhizosphere

Article 2015 Ziraat Fakültesi Global Advanced Research Journal of Agricultural Science4 ( 4 ) 146 Views
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Identification and Prevalence of Ralstonia Solanacearum From Potato Fields of Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed duri ...More

Article 2014 Ziraat Fakültesi MANAS Journal of Agriculture, Veterinary and Life Science (MJAVL)4 ( 1 ) 65 Views
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Endophytic fungi diversity of wild terrestrial plants in Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

A total of 255 species of wild medicinal plants were analyzed in Northern and Northeastern Kyrgyzstan; from 150 plant species, 278 endophytic fungal isolates were identified. Endophytic fungi were frequently recorded on members of large plant families: Asteraceae, Ranunculaceae, Rutaceae, Fabaceae,Lamiaceae, Boraginaceae, Moraceae, Iridaceae, Euphorbiaceae and Brassicaceae. In the foothill ecosystems, rich biodiversity and numerous species of endophytic fungi genera - Fusarium, Acremonium, Cladosporium, Penicillium, Curvularia, Monilia, Rhizoctonia, Papularia, Botrytis and Stemphylium - were r ...More

Article 2014 Ziraat Fakültesi Global Advanced Research Journal of Microbiology3 ( 10 ) 65 Views
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Alkane monooxygenase diversity recovered from Central Asia sediments

Tinatin DOOLOTKELDİEVA

Previous studies in our lab and elsewhere showed that different microbial gene groups are obtained from geographically distant sites. Alkane monooxygenases activate terminal carbon-carbon bonds in the first step of alkane degradation with the addition of an oxygen to the compound, resulting in an alcohol product. The aim of this study was to test the hypothesis that unique microbial populations and alkane monooxygenase (alkB and Cytochrome P450) genes can be recovered from geographically distinct sites, with varying degrees of anthropogenic impact. Sediment was collected from six river and str ...More

Chapter of Books 2012 Ziraat Fakültesi Diversity of biodegradative gene populations in aquatic sediments examined by gene-targeted metagenomics ( Chapter 3 ) 55 Views
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Scab Disease Caused by Venturia inaequalis on Apple Trees in Kyrgyzstan and Biological Agents to Control This Disease

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Among the most economically important fruit crops in South and North Kyrgyzstan affected by scab disease are apples (Malus domestica). Natural isolates of Venturia inaequalis were isolated from diseased parts of apple trees, selected from different regions, through sedimentation of air microflora on the crowns of fruit trees. Two biocontrol agents as Trichoderma viride and Streptomyces sр. were tested for apple scab control in vitro and field conditions. Two applications of Trichoderma viride within 35 days completely stopped the scab disease in seedling leaves. Within 40 days, the seedlings w ...More

Article 2017 Ziraat Fakültesi Advances in Microbiology7 ( 6 ) 60 Views
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