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Databases : Ulakbim (DergiPark) ✕Researcher : Saykal BOBUŞOVA ✕
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Dr. Saykal BOBUŞOVAZiraat Fakültesi
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Bacterial Community Structure In Petroleum Contaminated And Uncontaminated Soils Of Mountain Ecosystem

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

We compared a microbial diversity in uncontaminated and contaminated with petroleum soils in mountain ecosystems in Kyrgyzstan. Culture-dependent and culture - independent methods were used to analyze microbial diversity. PCR primers that target conserved iron binding motifs in alkane monooxygenase (alkB) and cytochrome P450 alkane hydroxylase were used to analyze genes of alkane degrading bacteria in chronically contaminated and uncontaminated sites, using an enrichment culture with dodecane. Analysis of 16S rRNA showed that in contaminated soils BetaProteobacteria phylum populations were abu ...More

Article 2015 Ziraat Fakültesi MANAS Journal of Agriculture, Veterinary and Life Science (MJAVL)5 ( 1 ) 57 Views
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Identification and Prevalence of Ralstonia Solanacearum From Potato Fields of Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed duri ...More

Article 2014 Ziraat Fakültesi MANAS Journal of Agriculture, Veterinary and Life Science (MJAVL)4 ( 1 ) 65 Views
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