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Construction, characteristics and high throughput molecular screening methodologies in some special breeding populations: a horticultural perspective

Hasan CAN | İbrahim İlker ÖZYİĞİT | Mustafa PAKSOY

Advanced marker technologies are widely used for evaluation of genetic diversity in cultivated crops, wild ancestors, landraces or any special plant genotypes. Developing agricultural cultivars requires the following steps: (i) determining desired characteristics to be improved, (ii) screening genetic resources to help find a superior cultivar, (iii) intercrossing selected individuals, (iv) generating genetically hybrid populations and screening them for agro-morphological or molecular traits, (v) evaluating the superior cultivar candidates, (vi) testing field performance at different location ...More

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Heavy Metal Levels and Mineral Nutrient Status of Natural Walnut (Juglans regia L.) Populations in Kyrgyzstan: Nutritional Values of Kernels

Bakıt BORKOYEV | Kalıypa SALİYEVA | Ali Osman SOLAK | İbrahim İlker ÖZYİĞİT

In this study, mineral nutrient and heavy metal (Al, Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn) contents of the walnut kernels and their co-located soil samples collected from the four different zones of natural walnut forests (Sary-Chelek, Arslanbap, and Kara-Alma in Jalal-Abad Region and Kara-Shoro in Osh Region) in Kyrgyzstan were investigated. The highest concentrations for all elements determined in the soil samples were observed in the Sary-Chelek zone whereas the Arslanbap zone was found to be having the lowest concentrations except Fe and Zn. The highest concentrations in the kerne ...More

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DNA fingerprinting and assessment of some physiological changes in Al-induced Bryophyllum daigremontianum clones

İbrahim İlker ÖZYİĞİT

Aluminum (Al) is one of the most important stress factors that reduce plant productivity in acidic soils. Present work thereby analyzed Al-induced genomic alterations in Bryophyllum daigremontianum clones using RAPD and ISSR markers, and investigated responding changes in photosynthetic pigment (chlorophyll a, b, a/b, total chlorophyll and carotenoid) contents and total soluble protein amounts in plant leaves. The main reason for the use of bulbiferous spurs originated clone plants was to increase reliability and acceptability of RAPD and ISSR techniques in DNA fingerprinting. Raised 40 clone ...More

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Construction, characteristics and high throughput molecular screening methodologies in some special breeding populations: a horticultural perspective

Hasan CAN | İbrahim İlker ÖZYİĞİT | Mustafa PAKSOY

Advanced marker technologies are widely used for evaluation of genetic diversity in cultivated crops, wild ancestors, landraces or any special plant genotypes. Developing agricultural cultivars requires the following steps: (i) determining desired characteristics to be improved, (ii) screening genetic resources to help find a superior cultivar, (iii) intercrossing selected individuals, (iv) generating genetically hybrid populations and screening them for agro-morphological or molecular traits, (v) evaluating the superior cultivar candidates, (vi) testing field performance at different location ...More

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Heavy Metal Levels and Mineral Nutrient Status in Different Parts of Various Medicinal Plants Collected from Eastern Mediterranean Region of Turkey

İbrahim İlker ÖZYİĞİT

Medicinal plants have been used for treatment of many diseases since the ancient times with traditional knowledge being transferred from generation to generation. However, in recent years, many natural habitats have been contaminated due to increased anthropogenic activities. Plants which are exposed to heavy metal toxicity may experience several serious problems. Furthermore, the inclusion of these plants into the food chain poses a threat to human health as well. Additionally, presence of heavy metals directly effect mineral nutrition and consequently the food quality. The aim of this study ...More

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Heavy metals and trace elements detected in the leaves of medicinal plants collected in the southeast part of Turkey

İbrahim İlker ÖZYİĞİT

The present study focused on the heavy metal and essential element contents that were detected in 37 different plants for making assessments to reveal possible heavy metal contamination and its effects on plant and human health. These plants having medicinal properties are used widely in the southeast part of Turkey. ICP-OES was employed for the determination of Al, B, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn concentrations in the leaves of some selected medicinal plants. The concentration rates (in mg kg(-1)) obtained in ascending order were as in the following ranges: 8.68-278.83 fo ...More

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Multidimensional Scaling of the Mineral Nutrient Status and Health Risk Assessment of Commonly Consumed Fruity Vegetables Marketed in Kyrgyzstan

Hasan CAN | İbrahim İlker ÖZYİĞİT

Intensive production of fruits and vegetables causes heavy metal accumulation. Consumption of this kind of foodstuff is a growing concern of the modern world with the additional distress of the supply of enough foodstuffs. To contribute to this global purpose, this research aimed to find out the mineral nutrient and heavy metal concentrations of commonly consumed fruity vegetables in Kyrgyzstan. Totally, ten different fruity type vegetables were collected from five different large bazaars of Kyrgyzstan. From these, 20 samples, including washed/unwashed rinds of vegetables, were quantified in t ...More

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Gene transfer to plants by electroporation: methods and applications

İbrahim İlker ÖZYİĞİT

Developing gene transfer technologies enables the genetic manipulation of the living organisms more efficiently. The methods used for gene transfer fall into two main categories; natural and artificial transformation. The natural methods include the conjugation, transposition, bacterial transformation as well as phage and retroviral transductions, contain the physical methods whereas the artificial methods can physically alter and transfer genes from one to another organisms

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Environment-Based Impairment in Mineral Nutrient Status and Heavy Metal Contents of Commonly Consumed Leafy Vegetables Marketed in Kyrgyzstan: a Case Study for Health Risk Assessment

Hasan CAN | İbrahim İlker ÖZYİĞİT

Leafy vegetables are important components of the human diet for providing mineral nutrients. However, due to the tendency of metal accumulation, metal contents of leafy vegetables need not only to be determined but also estimated health risk for revealing possible health effects on humans. The aims of this study are (I) to examine comprehensive concentrations of trace/heavy metals along with some macroelements including Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn in selected leafy vegetables from Kyrgyzstan; (II) to assess recommended dietary allowances (RDA); and (III) to evaluate hazard qu ...More

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Phytoremediation using genetically engineered plants to remove metals: a review

İbrahim İlker ÖZYİĞİT | Hasan CAN

Contamination by heavy metals including As, Cd, Co, Cu, Fe, Hg, Mn, Ni and Zn in agricultural fields is a global safety issue. Indeed, excessive accumulations of metals have detrimental effects on life by altering cell components such as lipids, proteins, enzymes and DNA. Phytoremediation appears as a solution to remove metals from contaminated sites, yet metal uptake is usually low in most common plants. Therefore, genetically engineered plants have been designed for higher efficiency of metal accumulation. Here, we review metal phytoremediation by genetically engineered plants with focus on ...More

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Particle bombardment technology and its applications in plants

İbrahim İlker ÖZYİĞİT

Particle bombardment, or biolistics, has emerged as an excellent alternative approach for plant genetic transformation which circumvents the limitations of Agrobacterium-mediated genetic transformation. The method has no biological constraints and can transform a wide range of plant species. Besides, it has been the most efficient way to achieve organelle transformation (for both chloroplasts and mitochondria) so far. Along with the recent advances in genome editing technologies, conventional gene delivery tools are now being repurposed to deliver targeted gene editing reagents into the plants ...More

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