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The aim of this study was to determine the normal hematological and biochemical parameters of the Thoroughbred Appaloosa horse breeds which reared in Tong and Issık-Kul regions of Kyrgyzstan. Fifty-six (n 56) healthy horse breeds in different ages (0–12 months and 1–5 years) and gender (n 29 male and n 27 female) were clinically examined and blood samples were taken. Red blood cell (RBC) count, white blood cell (WBC) count, the count (#); the percentage () of the lymphocytes (lymph), monocytes (mid), granulocytes (gran); and the values of hemoglobin (HGB), hematocrit (HCT), blood clot cell ...More
Background: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxoplasmosis form in visceral organs such as the liver, lung, and spleen. Some epidemiological studies have reported an association of T. gondii infection with liver cirrhosis. Methods. Acute infection was induced in fifteen 30-day-old normal Swiss albino mice. The mice were infected by intraperitoneal ...More
In this study, the presence, prevalence, and genotypes of Anaplasma phagocytophilum, A. ovis, and A. capra in sheep were investigated based on 16 S SSU rRNA, groEL, and gtlA gene-specific polymerase chain reaction (PCR), respectively. The sequences of the genes were used for detection of the phylogenetic position of the species. Additionally, a restriction fragment length polymorphism (RFLP) were carried out for discrimination of A. phagocytophilum and related variants (A. phagocytophilum-like 1 and 2). The prevalence of Anaplasma spp. was found as 25.8 (101/391), while it was found that A. ov ...More
Hemoplasma species cause clinical infections ranging from asymptomatic to life-threatening in cattle. To date, two hemoplasma species, Mycoplasma wenyonii (M. wenyonii) and Candidatus Mycoplasma haemobos (Ca. M. haemobos), have been identified from cattle. There is no data on the presence and distributions of bovine hemoplasma species in cattle in Kyrgyzstan, notwithstanding their potential negative economic impact on cattle production industries. In the current study, we investigated the molecular presence and distributions of bovine hemoplasma species from 358 cattle samples obtained from di ...More
Bovine piroplasmosis is a tick-borne disease caused by apicomplexan hemoparasites of the genera Theileria and Babesia. This study was carried out to assess the presence and frequency of piroplasm parasites in apparently healthy cattle in Kyrgyzstan. A total of 454 blood samples were collected from animals of various ages in eight villages located in the Chu valley and around the Lake Issyk Kul. The hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene was amplified with a set of primers specific targeting members of the genera Theileria and Babesia. Amplified PCR products were hybridize ...More
Background Dirofilaria immitisandDirofilaria repensare the causative agents of cardiopulmonary and subcutaneous dirofilariosis, respectively. This neglected disease mainly seen in dogs, cats and wild carnivores is re-emerging recent years. No study was conducted on dirofilariosis in dogs in Kyrgyzstan. Purpose The goal of this study was to investigateDirofilariaspecies using PCR and sequencing in dogs from Kyrgyzstan. Method Dirofilariaspp. infection in dogs was screened via convential PCR and sequencing in 337 dogs from Kyrgyzstan. Result The overall prevalence ofDirofilariaspp. was 0.59% (2/ ...More