Characterization of cereal cyst nematodes in wheat using morphometrics, SCAR markers, RFLP, and rDNA-ITS sequence analyses

Şenol YILDIZ

In this study, morphological and molecular characterizations of twenty-four Heterodera populations (cereal cyst nematodes, CCNs) collected from wheat production fields in Turkey were carried out. Light microscopy, species-specific markers, RFLP, and ITS sequencing were used to identify the nematode populations. The obtained CCN populations were identified as Heterodera avenae, H. filipjevi, and H. latipons according to the morphometric analysis, which was confirmed by the molecular techniques. The ITS region sequencing analysis confirmed the species identification, and phylogenetic analysis of ...More

An app for apples: Citizen-led mapping of fire blight in Central Asia

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Fire blight, caused by the bacterial pathogen Erwinia amylovora, is a severe bacterial disease of apple and pear that can quickly destroy whole plants. In the last decade, it was also detected in Central Asia, where wild pomaceous fruit plants represent the dominant species in mid-altitude forests and constitute a critical foundation for the entire ecosystem. Efficiently informing farmers, forestry services and private persons about the instances and dangers of fire blight, the correct way to recognize the symptoms, and the methods of disease control is thus of paramount importance in a vast a ...More

First report of Penicillium expansum causing postharvest fruit rot on pear in Kyrgyzstan

İsmail ERPER | Sezim COLDOŞBEKOVA

Keywords; penicillium fruit rot; postharvest; pyrus communis; RPB2

First report of damping-off on melon seedlings caused by Rhizoctonia solani AG 4 HG-II in Kyrgyzstan

İsmail ERPER

Potential of Turkish Beauveria bassiana isolates for the management of the polyphagous planthopper, Orosanga japonica Melichar 1898 (Hemiptera: Ricaniidae)

İsmail ERPER

Background Entomopathogenic fungi are a potential biological control agent for many pests. In this study, 14 native Beauveria bassiana isolates were molecularly identified and assessed for their virulence and mortality against adults of the polyphagous planthopper Orosanga japonica, Melichar (Hemiptera: Ricaniidae) a polyphagous sap-feeding insect, under laboratory conditions. Results Isolates obtained from naturally infected adults O. japonica were molecularly identified as B. bassiana by sequencing the internal transcribed spacer and 26S large subunit of ribosomal DNA. In the bioassay, the r ...More

Tuta absoluta continues to disperse in Asia: damage, ongoing management and future challenges

Tair Esenalı Uulu

Since its initial detection in Turkey in 2009, the invasive destructive pest South American tomato pinworm Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) has started its new invasion journey to east and invaded most Asian countries. This pest currently causes extensive damage to tomato production and potentially threatens key production areas such as China. To provide an overview of current status of T. absoluta in Asia, we have briefly reviewed the damage and economic impacts by this pest locally and discussed why this species has spread so rapidly among the countries. Moreover, ongoing i ...More

First report of charcoal rot caused by Macrophomina phaseolina on kiwifruit in Turkey

İsmail ERPER

During June and August in 2018, kiwifruit (cv. Hayward) plants exhibiting symptoms of charcoal rot including chlorosis, necrosis, leaf shedding, root rot, and trunk dry rot were observed with an incidence of up 5?n three commercial orchards located in Altınordu, Fatsa and Perşembe districts of Ordu province. After surface sterilization with 1?aClO, small pieces excised from root tissues of diseased plants were rinsed twice in sterile distilled water, dried on sterile filter papers and aseptically transferred to plates containing potato dextrose agar. Ten isolates from the plates were identifie ...More

Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Fire Blight, an economically relevant disease of apple, pear, and quince trees that is caused by the Gram-negative bacterium Erwinia amylovora, was first reported from Kyrgyzstan in 2008. One decade later, the disease has spread across the northern part of the country, affecting fruit orchards mainly in Chuy and Issyk-kul regions. Using semi-selective cultural media, bacteria have been isolated from plant material sampled in infested orchards from different locations in Kyrgyzstan, and 16S rRNA gene sequence determination together with diagnostic PCR have been used to identify E. amylovora bac ...More

Genetic Diversity in Apple Accessions Belong to Different Species Collected from Natural Populations of Tianshan Mountains, South-West Kyrgyzstan

Abdıkerim ABDULLAYEV

There isn't any concrete consensus about the origin of domesticated apple. However, it is generally thought that the origin of this apple primarily comes from M. sieversii, also known as Central Asia wild apple. The second significant contribution is thought to be supplied by M. sylvestris. Central Asia is an important origin region for apples. Within this region, mountainous and forestlands of Kyrgyzstan constitute a significant source for wild apple species. The present study was conducted to put forth the genetic relationships between 65 accessions belonging to M. kirgishorum, M. domestica, ...More

Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities

Tinatin DOOLOTKELDİEVA | Mahabat KONURBAYEVA | Saykal BOBUŞOVA

In Kyrgyzstan, many former storehouses and dump sites for obsolete pesticides exist. In 2009/2010, an inventory and assessment of these sites including risks of environmental hazard has been conducted by FAO and the World Bank. Monitoring revealed high concentration of pesticides listed as persistent organic pollutants (POPs). The purpose of this research was to study the microbial structural complexes of the pesticide-contaminated soils in these dumping zones, and to search for and select microorganism’s destructors with cytochrome P450 genes for pesticide degradation. Culture-dependent and c ...More

Control of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in open field tomato crops using the mating disruption technique

Tair Esenalı Uulu

This study was conducted in Meram district of Konya province (Turkey) in 2018 and 2019 to evaluate the efficacy of mating disruption against the tomato leafminer, Tuta absoluta in an open-field tomato growing area. Three pheromone-treated fields and three control fields were used to evaluate the effectiveness of mating disruption in both years. In pheromone-treated fields, mating disruption pheromone was applied once at a rate of 2500 g/ha with a caulking gun. In control fields, only pheromone traps were hung for monitoring and comparing the pest population. Male captures in monitoring pheromo ...More

First report of Rhizoctonia solani AG 1-IB causing root and stem rot of kale (Brassica oleracea var. acephala) in Turkey

İsmail ERPER