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This work presents Fe3O4 and AgFe nanoparticles with an average diameter of 25 and 15 nm synthesized by chemical reduction of corresponding salts under a mild condition. Cubic crystal structure and spherical shape of the nanoparticles were studied by X-ray diffraction, Field emission SEM and energy-dispersive spectroscopy analysis. For biomedical applications, the nanoparticles were tested against bacteria E.coli and results revealed AgFe nanoparticles