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Source : Pakistan Veterinary ... ✕Faculty / Institute : Veteriner Fakültesi ✕
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Quantification of Mitral Regurgitation in Anatolian Shepherd Dogs with Asymptomatic Degenerative Mitral Valve Disease

Hasan GÜZELBEKTEŞ

Degenerative mitral valvular disease (DMVD) is the most frequent cardiac disease, causing mitral regurgitation (MR) in dogs. The purpose of this study was to compare the ratio of the regurgitant jet area (RJA) to the left atrial area (LAA) (RJA/LAA) with subtracting method to quantify regurgitant volume (RegV) and regurgitant fraction (RF) in asymptomatic Anatolian Shepherd Dogs (ASHs) with DMVD. Thirty-eight ASHs with DMVD were used as experimental group. The control group consisted of 35 healthy ASHs. In 38 ASHs with DMVD (20 B1 dogs and 18 B2 dogs), the severity of MR was assessed by RJA/L ...More

Article 2016 Veteriner Fakültesi Pakistan Veterinary Journal36 ( 4 ) 81 Views
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Effect of different storage temperatures on the stability of bovine viral diarrhea virus RNA in blood samples

Orhan YAPICI

The present study was conducted to determine stability of Bovine viral diarrhea virus (BVDV) RNA stored at different temperatures. A total of 6 blood samples obtained from a private cattle farm, which were found to be antigen positive (Ag+) by direct ELISA method, were used in this study. BVDV Ag+ samples were stored separately at 4, 21 and 37oC for 1 month. The samples were analyzed on the 0, 1st, 2nd, 3rd and 4th weeks by ELISA for the presence of BVDV Ag and by RT-PCR for the presence of BVDV RNA. Stability of BVDV RNA was calculated using maximum concentration (Cmax) and area under the cur ...More

Article 2015 Veteriner Fakültesi Pakistan Veterinary Journal35 ( 4 ) 59 Views
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