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Publication year : 2021 ✕Researcher : Tinatin DOOLOTKELDİE ... ✕
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Prof. Dr. Tinatin DOOLOTKELDİEVAZiraat Fakültesi
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The Improving Conditions for the Aerobic Bacteria Performing the Degradation of Obsolete Pesticides in Polluted Soils

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Currently, in the territory of Kyrgyzstan, 50 storage facilities of obsolete pesticides exist; they store about 5000 tons of these hazardous chemicals. The storage conditions have become unusable for a long time. They pose a serious threat to the people living there, livestock, and the environment. The main purpose of this research was the use of selected bacteria with cytochrome P450 genes for the bioremediation of polluted soils around the burial sites in model soil experiments. In the first trial of biodegradation experiments, one contaminated soil was used without any changes in chemical c ...More

Article 2021 Ziraat Fakültesi Air, Soil and Water Research14 150 Views
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Genetic characterization of Erwinia amylovora isolates detected in the wild walnut-fruit forest of South Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Erwinia amylovora, the causal agent of fire blight, was first reported in Kyrgyz orchards in 2008 and, within a few years, the disease has spread throughout most of the northern part of the country. As fire blight expanded and penetrated new areas, it also reached the unique ecosystem constituted by the Arslonbob wild walnut-fruit forests in South Kyrgyzstan. Here, we present the results of field surveys in the forests and in the nearby orchards and gardens of the Jalal-Abad region during the 2019 season. Further, we use genetic profiling of the CRISPR regions to compare the genotypes of the o ...More

Article 2021 Ziraat Fakültesi Journal of Plant Pathology103 ( SUPPL 1 ) 47 Views
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Isolation, Identification, and Characterization of the Nematophagous Fungus Arthrobotrys oligospora from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Ayperi MURATBEKOVA

Purpose Predatory fungi have been the subject of fundamental studies and their potential as biological control agents against parasitic plant nematodes has been assessed. The aim of the present study was to isolate and identify predatory fungi, performing in vitro and in vivo screening to select highly active strains to control parasitic nematodes. Methods Different nutrient media were used to isolate predatory fungi and determine their morphological and cultural properties. Identification was performed by classical and molecular biology methods. In vitro and in vivo screening was conducted to ...More

Article 2021 Ziraat Fakültesi Acta Parasitologica66 ( 4 ) 95 Views
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Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer

Tinatin DOOLOTKELDİEVA

Fire blight is the most devastating disease affecting pome fruit production globally. The pathogen is native to North America and was imported to western Europe in the 1950s, progressively spreading over the continent in the ensuing decades. Previous phylogenetic studies have revealed the extreme genetic homogeneity of the pathogen outside its center of origin, which makes epidemiological studies difficult. These are generally only possible using hypervariable regions of the genome such as those represented by CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), which are, howe ...More

Article 2021 Ziraat Fakültesi Phytopathology Research3 ( 1 ) 102 Views
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