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An electrochemical biosensor was developed for the determination of Escherichia coli (E. coli) in water. For this purpose, silver-gold core-shell (Ag@Au) bioconjugates and anti-E. coli modified PS-microwells were designed in a sandwich-type format in order to obtain higher sensitivity and selectivity. Ag@Au bimetallic nanoparticles were synthesized by co-reduction method. The core-shell formation was analyzed by using UV-Vis spectroscopy and transmission electron microscopy. Biotin labeled anti-E. coli antibodies were coupled with Ag@Au nanoparticles to form bioconjugates. The electrochemical ...More
An updated checklist of the species of Turkish Raphidioptera and further analysis concerning their zoogeographical distribution are presented in this study. The present checklist has been based on studies from the literature and includes records from extensive material that I have identified over the last few years. The Turkish Raphidioptera presently comprises 40 species in 8 genera and 2 families. There are 38 species of Raphidiidae, while there are only 2 species of Inocelliidae in Turkey. Among these species, 19 species are recorded as endemic and the endemism rate is 47.5%. Distributions ...More
Zeki SEVEROĞLU | İlhan DOĞAN | Gülbübü KURMANBEKOVA
Uncontrolled and unplanned urbanization and industrialization due to increase of population and rapid industrial development have created severe environmental problems in Kyrgyzstan during the last few decades. In this study, Juniperus virginiana, a dioecious species, was employed in order to make assessment of the heavy metal pollution rate in the area and of the heavy metal pollution impact on the mineral nutrient status of the plant. For this study, leaf (washed and unwashed) and bark samples of J. virginiana, and its co-located soil samples were collected from eight different stations, all ...More
The present work investigated the effect of long-term stack-storage after harvesting on selected chemical and rheological properties of Kyrgyz rice cultivars, Ozgon. The performance indicators of in stack-stored Ozgon rice samples after 4, 6, and 12 days was grain size, grain weight, color, moisture, total starch, amylose, protein, fat and ash contents, and mineral composition as well as the rheological properties of rice flour dough. The change of stored rice is caused by the organic modification of the starch structure, decreasing of moisture as well as proteolysis of proteins in the endospe ...More
The gradual Cl replacement reactions of NN (1–3) or NO spirocyclic monoferrocenyl cyclotriphosphazenes (4 and 5) with the potassium salt of 4-hydroxy-3-methoxybenzaldehyde (potassium vanillinate) resulted in the mono (1a–5a), geminal (gem-1b–5b), non-geminal (cis-5b and trans -1b–4b), tri (1c, 3c–5c) and tetra-vanillinato-substituted phosphazenes (1d–5d). All the phosphazene derivatives have stereogenic P-center(s), except tetra-substituted ones. The vanillinatophosphazenes have reversible voltammograms with one-electron anodic and cathodic peaks which are attributed to ferrocenyl redox probe. ...More
The present study was conducted to determine stability of Bovine viral diarrhea virus (BVDV) RNA stored at different temperatures. A total of 6 blood samples obtained from a private cattle farm, which were found to be antigen positive (Ag+) by direct ELISA method, were used in this study. BVDV Ag+ samples were stored separately at 4, 21 and 37oC for 1 month. The samples were analyzed on the 0, 1st, 2nd, 3rd and 4th weeks by ELISA for the presence of BVDV Ag and by RT-PCR for the presence of BVDV RNA. Stability of BVDV RNA was calculated using maximum concentration (Cmax) and area under the cur ...More
Species composition and distribution of intestinal ciliates were investigated in the feces from 15 domestic horses living in Bishkek, Kyrgyzstan. Twenty-three species belonging to 14 genera were identified. This is the first study on intestinal ciliates in domestic horses living in Kyrgyzstan. The mean number of ciliates was 14.1 +/- 6.8 x 10(4) cells ml(-1) of feces and the mean number of ciliate species per host was 6.0 +/- 3.2. No endemic or new species were detected. Blepharocorys was the major genus as these ciliates were detected in high proportions. In contrast Holophryoides, Allantosom ...More
Bu çalışma, gebeliğin farklı dönemlerindeki fare desidua bazalis dokusunda periyodik-asit Schiff (PAS)-pozitif granüllere sahip uterus doğal katil (uNK) hücrelerinin dağılımının belirlenmesi amacıyla yapıldı. Bu amaçla, 12-14 haftalık fareler, gebe olmayan-kontrol grubu ile gebeliğin birinci, ikinci ve üçüncü haftalarının ortasına karşılık gelecek şekilde erken, orta ve geç gebelik dönemi (sırasıyla gebeliğin 3, 10. ve 17. günleri) olmak üzere 4 gruba ayrıldılar (n= 6). Plasentasyonun ilerlemesiyle birlikte metriyal bölge (MLAp; mesometrial lymphoid aggregate of pregnancy), desidua bazalis, ba ...More
The presented research is about characterization of Cellulose Triacetate (CTA) based Polymer Inclusion Membranes (PIMs) which incorporated the commercial extractant Aliquat 336, Tributylphosphate (TBP) as modifier and 2-Nitro Phenyl Pentyl Ether (NPPE) as plasticizer, for the preparation of the membranes. Chemical and physical characteristics of the synthesized membranes especially membrane thickness and side difference effects were investigated. Different surface structures and membrane thickness affect the extraction efficiency of membranes. Membrane extraction experiments were studied where ...More
The present stu dy is aimed at comparing the effects of ice massage versus cold-water immersion on creatinekinase (CK) levels and delayed-onset muscle soreness (DOMS) values of elite wrestlers. To ac hieve the objectives of the research twenty elite wrestlers participated in the stu dy. Participants were separ ated into two groups (ice massage vs. cold water immersion) of whose weights were equal. Between the sets, experim ent group was applied ice message for 8 minu tes while control group was given cold-water immersion. Blood samples of the subjects were tak en before and after the exercises ...More
The vascular flora alongside the railway track between Haydarpasa and Gebze in Turkey was investigated and the floristic features of the study area are presented here. The investigation was undertaken during 2003-2009. In total 194 plant taxa were determined. Out of these 174 were naturally growing and 20 were exotics and cultivated. Native taxa within the flora belonged to 135 genera and 50 families, exotic and cultivated taxa belonged to 20 genera and 15 families. The members of Dicotyledonae constituted of 82.76% and Monocotyledonae 16.09% of the native taxa. The highest number of these tax ...More
The purpose of this study was to investigate the effects of sports drinks (isotonic) and water consumptions of the elite wrestlers during the exercise. Eighteen-trained elite wrestlers participated in this study. The subjects were divided into two groups according to their body-weight and were also given either water or isotonic sports drink. Water and sports drinks get rid of the dehydration during the trainings (P>0.05). Blood samples were taken before and after the exercises,and were analyzed for Sodium (Na), Potassium (K) and Chlorine (Cl). Body temperatures of subjects were measured as ºC ...More