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Publication year : 2014 ✕Faculty / Institute : Ziraat Fakültesi ✕
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Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Ali İrfan İLBAŞ

Plant tissue culture is an efficient technique for conserving endemic plant species. A reproducible in vitro regeneration protocol was developed for the endemic Iris sari and Iris schachtii in the present study. The highest number of shoots per explant was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L thidiazuron (TDZ) plus 0.5 mg/L α-naphthaleneacetic acid (NAA) and 1 mg/L TDZ plus 0.5 mg/L NAA, whereby 96.88% and 100% shoot induction with 9.55 and 11.34 shoots per explant of Iris sari and Iris schachtii were recorded, respectively. Regenerated shoots were successfull ...More

Article 2014 Ziraat Fakültesi Turkish Journal of Agriculture and Forestry38 ( 3 ) 103 Views
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Identification and Prevalence of Ralstonia Solanacearum From Potato Fields of Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed duri ...More

Article 2014 Ziraat Fakültesi MANAS Journal of Agriculture, Veterinary and Life Science (MJAVL)4 ( 1 ) 65 Views
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Endophytic fungi diversity of wild terrestrial plants in Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

A total of 255 species of wild medicinal plants were analyzed in Northern and Northeastern Kyrgyzstan; from 150 plant species, 278 endophytic fungal isolates were identified. Endophytic fungi were frequently recorded on members of large plant families: Asteraceae, Ranunculaceae, Rutaceae, Fabaceae,Lamiaceae, Boraginaceae, Moraceae, Iridaceae, Euphorbiaceae and Brassicaceae. In the foothill ecosystems, rich biodiversity and numerous species of endophytic fungi genera - Fusarium, Acremonium, Cladosporium, Penicillium, Curvularia, Monilia, Rhizoctonia, Papularia, Botrytis and Stemphylium - were r ...More

Article 2014 Ziraat Fakültesi Global Advanced Research Journal of Microbiology3 ( 10 ) 65 Views
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