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The aims of this study were to determine the factors that affect follicle size at the time of artificial insemination (AI) and to detect the relationship between ovulatory follicle size at the time of AI and pregnancy rate in dairy cows. A total of 1428 follicle size measurements were obtained from Holstein-Friesian (HF) and Swedish Red (SR) cows that were inseminated with the fixed-timed AI protocols used in this study. Follicle size was affected by breed (P - 0.0001), milk production (P - 0.01), parity (P - 0.05), and season (P = 0.04). Follicle size was greater (P = 0.0001) in the HF (15.55 ...More
The aim of this study was to determine the effects of three different dietary starch levels on postpartum milk yield, blood metabolites, and reproductive traits in cows. A total of 23 primiparous Holstein-Friesian cows were fed three different diets including different starch levels on a dry matter basis from parturition until day 80. Cows were randomly assigned into the three groups according to the starch levels: low (LS, 16%, n = 7), medium (MS, 20%, n = 8), and high (HS, 24%, n = 8). Milk yields were recorded daily, and body condition score (BCS) was determined every week. Transrectal ultr ...More
The fields in which lead (Pb) finds application in the modern world have increased dramatically in recent years. As a consequence of this intensive utilization of Pb, its toxicity tends to pose more and more environmental problems. The aim of this study was to evaluate the genotoxic potential of Pb and to characterize some physiological parameters in Secale cereale under Pb stress. Plants were subjected to different exposure levels of Pb (0, 100, 200 and 400 µmol/L) for two weeks. At the end of the experimental period, the effects of Pb exposure on the photosynthetic pigments content (chloroph ...More
Graphene oxide (GO) was covalently attached to glassy carbon (GC) electrode (GC–O–GO) for fabricating nanosensors to determine trace Pb2+ and Cd2+ using differential pulse anodic stripping voltammetry (DPASV). Surface characterization of the nanofilm-covered electrode was performed via electrochemical cyclic voltammetry (CV), transmission electron microscopy (TEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) techniques. Surface pKa of the GO covalent attached GC (GC–O–GO) was calculated via CV. Under optimal conditions, a linear response was found for Pb2+ and Cd2 ...More
Highly sensitive 3,8-diaminobenzo[c]cinnoline (3,8-DABCC) modified graphene oxide (GO) electrode was prepared, and it was denoted as pGO-DABCC. The electrode material was characterized with infrared spectroscopy (IR), X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). pGO-DABCC on glassy carbon (GC/pGO-DABCC) electrode was investigated for simultaneous determination of Cd2+ and Pb2+ in aqueous media by differential pulse anodic stripping voltammetry (DPASV). Optimum conditions of the electrode were determined with some parameters ...More
Degenerative mitral valvular disease (DMVD) is the most frequent cardiac disease, causing mitral regurgitation (MR) in dogs. The purpose of this study was to compare the ratio of the regurgitant jet area (RJA) to the left atrial area (LAA) (RJA/LAA) with subtracting method to quantify regurgitant volume (RegV) and regurgitant fraction (RF) in asymptomatic Anatolian Shepherd Dogs (ASHs) with DMVD. Thirty-eight ASHs with DMVD were used as experimental group. The control group consisted of 35 healthy ASHs. In 38 ASHs with DMVD (20 B1 dogs and 18 B2 dogs), the severity of MR was assessed by RJA/L ...More
The aim of this study was to determine the clinical relevance of cardiac biomarkers [troponin I and T, creatine kinase-MB fraction (CK-MB) and lactate dehydrogenase (LDH)] in premature calves with respiratory distress syndrome. Seventy premature calves were admitted to the clinic within 24 h after birth. Respiratory distress syndrome was diagnosed in premature calves by clinical examination and venous blood gas analysis. Ten healthy calves, aged 5 days, were used as control. Cardiac troponin I and T were analysed using ELISA and ELFA, respectively. Serum CK-MB and LDH were also analysed in an ...More
On a farm in the Kyrgyz Republic, several dead sheep were found without any history of illness. The sheep showed several ulcerations on lips and bare-skinned areas. At necropsy the lungs showed multiple firm nodules, which were defined as pox nodules histologically. In the rumen hyperkeratotic plaques were visible. With electron microscopy pox viral particles were detected and confirmed with qPCR as Capripoxviruses. Although all members of the Capripoxvirus genus are eradicated in western countries, this study should remind us of the classical lesions observed in poxvirus infections.
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In the present study, virological and serological investigations were performed to determine the presence and prevalence of Canine corona virus (CCoV) infection in dog population in Turkey. Sera samples were analyzed for specific antibodies against CCoV by indirect enzyme linked immunosorbent assay (i-ELISA) while leukocyte samples were inoculated onto monolayers of Madin Darby Canine Kidney permanent cell culture. The cells were examined for viral antigen by direct immunofluorescence (IF) test after third passage. CCoV seropositivity was found in 46 (24.46%) of 188 dogs by indirect ELISA whil ...More
The purpose of the study reported here was to determine echocardiographic findings in naturally heartworm-infected dogs, and evaluate these findings to use in the diagnosis of adult worms in the pulmonary artery. Sixteen male dogs (8.8%) of the 180 dogs in our study, weighing 7.5-42.0 kg, ranging in age from 1 to 8 years, were determined to be positive for Dirofilaria immitis according to the results of membrane filtration and antigen ELISA tests. Dogs identified with adult heartworms in their blood were evaluated by parasitological. The mean worm burden in infected dogs was 4730 ± 5479 (min: ...More
This preliminary study was aimed at determining the occurrence of haemosporidian parasites in the blood of sparrowhawks. For this purpose, blood samples were prepared from 9 sparrowhawks. All birds had been collected from different areas of Northeast Turkey. Blood smears were stained with 5% Giemsa solution and examined under the light microscopy. A total of 7 individuals were infected (77.8%). Haemoproteus spp., Leucocytozoon spp. and Plasmodium spp. were detected in 5 (55.6%), 6 (66.7%) and 4 (44.4%) birds, respectively. There were no haemosporidian parasites in 2 birds (22.2%). We observed ...More
This current work aims to assess the efficacy and tolerability of trabeculectomy procedure for the management of congenital glaucoma in two cats. Filtration surgery was performed using a limbus-based approach, and flap was raised smoothly to nearly limbus on sclera. Sclera-corneal trabecular block was removed both with surgical knife and microsurgery scissors. Successful result was defined as resolution of leakage with no need for additional surgery, together with maintaining intraocular pressure in the range of 5-21 mmHg with anti-glaucoma medications. In conclusion, trabeculectomy decreased ...More