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Zero hungerEnd hunger, achieve food security and improved nutrition, and promote sustainable agriculture
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Trait profiling and genotype selection in oilseed rape using genotype by trait and genotype by yield*trait approaches

Hossein ZEİNALZADEH TABRİZİ

Selection and breeding for high-yielding in oilseed rape have always been one of the leading objectives for oilseed rape breeders. This process becomes more complicated when all quantitative traits are considered in selection in addition to grain yield. In the present study, 18 oilseed rape genotypes along with 2 check cultivars (RGS003 and Dalgan) were evaluated across 16 environments (a combination of 2 years and eight locations) in the tropical climate regions of Iran during 2018–2019 and 2019–2020 cropping seasons. The experiments were conducted in a format of randomized complete block des ...More

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Streptomyces bio-products against apple and pear diseases in organic orchards

Tinatin DOOLOTKELDİEVA

Erwinia amylovora as a fire blight pathogen and Venturia inaequalis as a scab pathogen were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical and pathogenicity tests, alongside PCR analyses were conducted to identify the local isolates of Erwinia amylovora and Venturia inaequalis. The alternative antagonistic microorganisms which combat bacterium E. amylovora and fungus Venturia inaequalis were tested within in vitro and in vivo conditions. The results revealed ...More

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Construction, characteristics and high throughput molecular screening methodologies in some special breeding populations: a horticultural perspective

Hasan CAN | İbrahim İlker ÖZYİĞİT | Mustafa PAKSOY

Advanced marker technologies are widely used for evaluation of genetic diversity in cultivated crops, wild ancestors, landraces or any special plant genotypes. Developing agricultural cultivars requires the following steps: (i) determining desired characteristics to be improved, (ii) screening genetic resources to help find a superior cultivar, (iii) intercrossing selected individuals, (iv) generating genetically hybrid populations and screening them for agro-morphological or molecular traits, (v) evaluating the superior cultivar candidates, (vi) testing field performance at different location ...More

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Occurrence, identification and phylogenetic analyses of cereal cyst nematodes in Kyrgyzstan

Şenol YILDIZ | Tair Esenalı Uulu

The study investigated the status of cereal cyst nematodes (CCN) in the main wheat-growing areas of Kyrgyzstan in 2020. Soil samples were taken from 69 different wheat fields located in Chuy and Issyk-Kul provinces. CCN were found in thirty-one out of the sixty-nine locations surveyed. The highest occurrence of CCN was in the Tyup location in Issyk-Kul province with 81 cysts (250 cm3 soil)-1. The CCN populations were identified by both morphological and molecular analyses. According to the results, all populations were identified as Heterodera filipjevi. No variations in rDNA-ITS sequencing da ...More

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Evaluation of wheat (Triticum aestivum L.) lines for drought tolerance in Kyrgyzstan

Venera İSAYEVA

The study was carried out to explore drought stress indices in F4 hybrid generations of Triticum aestivum L. to select drought stress tolerant lines for rainfed areas of Kyrgyzstan. Wheat is the main food crop in Kyrgyzstan. There are about 0.3 million ha allotted for wheat, more than half of these sown areas (0.2 million ha) are in rainfed farming zones, where the amount of precipitation rarely exceeds 300-400 mm per year. The study was conducted in 2019 at the experimental field of Agricultural Faculty of Kyrgyz-Turkish Manas University. Eighteen hybrid lines of spring wheat and two released ...More

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Nematode-citrus plant interactions: host preference, damage rate and molecular characterization of Citrus root nematode Tylenchulus semipenetrans

Şenol YILDIZ

center dot Citrus plants are host to several plant parasitic nematodes (PPNs), which are microscopic organisms. Among PPNs, the citrus root nematode, T. semipenetrans (Cobb 1913) (Tylenchida: Tylenchulidae), causes significant damage to citrus plantations worldwide. Understanding citrus nematode populations, precise identification, host preference among citrus species, and damage threshold are crucial to control T. semipenetrans. The minutiae of citrus plant-nematode interactions, nematode density and molecular nematode identification are not well understood. In this study, nematode species an ...More

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Identification of water stress-tolerant edible pumpkin seed (Cucurbita pepo) genotypes using seed yield-based tolerance indices

Atilla DURSUN

Pumpkin is usually cultivated in arid and semiarid regions, and the lack of water stress-tolerant cultivars is a major limiting factor. Therefore, this study was carried out to identify superior water stress-tolerant genotypes. For this purpose, 44 inbred lines with superior agronomic traits were selected from the gene pool. In addition, two hybrids (G1-Mert Bey F1 and G2-Sena Hanim F1) and two landraces (G3-Hatun Tırnağı and G4-Cercevelik) with high commercial value were used as commercial cultivars. The water stress indices were calculated from seed yields from the pumpkin genotypes grown in ...More

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Salt tolerance of endophytic root bacteria and their effects on seed germination and viability on tomato plants

Eren ÖZDEN

Salinity is one of the most brutal environmental factors limiting the productivity of agricultural lands worldwide. It is considered that the salinity may be one of the important reasons for the low yield in Igdir of the tomato plants, which is medium resistant (3-5 dS.m-1) among vegetables. Eco-friendly techniques such as endophytic root bacteria treatments (ERB) are needed to restore saline soils to agriculture and also to increase the yield of tomatoes. Endophytic bacteria colonizing the inside of plants increase plant growth by various mechanisms and also mitigate the adverse effects of bi ...More

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Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS se ...More

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Molecular evidence depicts genetic divergence among Agropyron elongatum and A. cristatum accessions from gene pool of Iran

Hossein ZEİNALZADEH TABRİZİ

The genus Agropyron has an important role in soil protection and forage production in rangelands. The investigation utilized 37 ISSR primers, resulting in the detection of 956 loci within the A. elongatum genome and 705 loci within the A. cristatum genome. The findings revealed a high level of polymorphism, with 97 of loci in A. elongatum and 84 of loci in A. cristatum exhibiting variability. Notably, the primer (AC)8GCT emerged as a promising candidate for evaluating genetic diversity due to its ability to amplify numerous loci in both species. Using both the UPGMA algorithm and Bayesian anal ...More

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Heritability, genetic advance and sequential path analysis of oil yield and related traits in spring oilseed rape genotypes

Hossein ZEİNALZADEH TABRİZİ

The efficiency of crop breeding programs is largely driven by the availability of genetic diversity and the inheritance of desirable traits. Heritability, genetic advance and sequential path analysis were assessed in 26 spring oilseed rape genotypes in a randomized complete block design with three replicates over two crop years 2018-2020 at the experimental field of Moghan Agricultural Research Station, Parsabad, Iran. Several morpho-physiological characteristics were recorded during the experiments. Genetic characteristics, heritability, and genetic advance in oilseed rape genotypes were esti ...More

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Identification of Microbial Populations Present in Agricultural and Nonarable Soils in the Talas Valley, Northern Kyrgyzstan, in Autumn

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Soil bacterial and fungal communities were investigated in relation to soil type and farm management practices after vegetation harvesting in autumn. Soils from fields cultivated with Phaseolus vulgaris (bean) and Pyrus comminus (pear) and nonarable, natural areas were studied. Microbial diversity was analysed using cultivation-dependent methods (isolation of pure cultures) and cultivation-independent methods (direct extraction of DNA from soil, followed by PCR amplification of the 16S rRNA and 18S rRNA genes). The use of cultivation-dependent methods revealed that there were no differences in ...More

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