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Life on landProtect, restore and promote sustainable use of terrestrial ecosystems; sustainably manage forests; combat desertification; halt and reverse land degradation; halt biodiversity loss
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Agrobacterium- mediated transformation of Turkish upland rice (Oryza sativa L.) for Dalapon herbicide tolerance

Yılmaz KAYA | İbrahim İlker ÖZYİĞİT | Ali ASLAN

Agrobacterium-mediated transformation of upland rice is established in few numbers of cultivars due to the high cultivar-specificity of regeneration from transformed explants. Further, dehalogenase E (dehE) gene had been characterized in Pseudomonas putida and it produces an enzyme that degrades dalapon. This study aimed to transform Turkish upland rice with the dehE herbicide resistant gene and addresses the challenges of transgenic rice recovery by identifying explant and transformation method. Constructed vector pCAMdehE carrying dehE gene was transferred into the rice shoot apex by Agrobac ...More

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Inhibitory effects of allelopathic crops on seedling growth of weed Hypericum triquetrifolium Turra

İbrahim İlker ÖZYİĞİT

In Laboratory bioassays and Pot culture, we determined the effects of aqueous extracts of 7-allelopathic crops [Alfalfa (Medicago sativa L.), Sorghum (Sorghum bicolor L.), Sunflower (Helianthus annuus L.), Rape (Brassica napus L.), Rye (Secale cereal L.), Barley (Hordeum vulgare L) and Oat (Avena saliva L)] on the seedling growth of weed Hypericum triquetrifolium. In first Lab. bioassay, barley and rape were found most phytotoxic to Hypericum seedlings growth. Then these two crops effects were further tested in pot experiments, with different residues rates (R1, R2, R3 for rape and B1, B2, B3 ...More

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GENETIC DIVERSITY AND PHYLOGENETIC ANALYSES OF TURKISH RICE VARIETIES REVEALED BY ISSR MARKERS AND CHLOROPLAST trnL-F REGION

İbrahim İlker ÖZYİĞİT

A set of 17 Turkish rice (Oryza sativa L.) varieties (TRVs) were characterized using inter simple sequence repeat (ISSR) markers to study genetic diversity. Also, the sequences of trnL((UAA))-F-(GAA) intergenic spacer (IGS) regions were used to evaluate the phylogenetic relationships of TRVs. According to ISSR data, the observed number of alleles (Na), effective number of alleles (Ne), Nei

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MINERAL NUTRIENT COMPOSITIONS OF FIELD-GROWN WEED AND MAIZE (Zea mays L.) PLANTS IN TERMS OF COMPETITION

İbrahim İlker ÖZYİĞİT

Amaranthus retroflexus L., Convolvulus arvensis L., Cynodon dactylon (L.) Pers. var. villosus, Datura stramonium L., Echinochloa crus-galli (L.) Beauv and Portulaca oleracea L. are weeds commonly seen in agricultural fields. Weeds pose serious threat to agricultural production. This study focuses on mineral nutrient uptake competition arisen between maize and weed species. Maize (Zea mays L.) was planted in a model field in Emirli Village-Istanbul/Turkey and the above-mentioned weeds were allowed to be grown along with maize. Then, weed and maize species along with their co-located soil sample ...More

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Phytoremediation using genetically engineered plants to remove metals: a review

İbrahim İlker ÖZYİĞİT | Hasan CAN

Contamination by heavy metals including As, Cd, Co, Cu, Fe, Hg, Mn, Ni and Zn in agricultural fields is a global safety issue. Indeed, excessive accumulations of metals have detrimental effects on life by altering cell components such as lipids, proteins, enzymes and DNA. Phytoremediation appears as a solution to remove metals from contaminated sites, yet metal uptake is usually low in most common plants. Therefore, genetically engineered plants have been designed for higher efficiency of metal accumulation. Here, we review metal phytoremediation by genetically engineered plants with focus on ...More

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Particle bombardment technology and its applications in plants

İbrahim İlker ÖZYİĞİT

Particle bombardment, or biolistics, has emerged as an excellent alternative approach for plant genetic transformation which circumvents the limitations of Agrobacterium-mediated genetic transformation. The method has no biological constraints and can transform a wide range of plant species. Besides, it has been the most efficient way to achieve organelle transformation (for both chloroplasts and mitochondria) so far. Along with the recent advances in genome editing technologies, conventional gene delivery tools are now being repurposed to deliver targeted gene editing reagents into the plants ...More

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