Bacterial Community Structure In Petroleum Contaminated And Uncontaminated Soils Of Mountain Ecosystem

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

We compared a microbial diversity in uncontaminated and contaminated with petroleum soils in mountain ecosystems in Kyrgyzstan. Culture-dependent and culture - independent methods were used to analyze microbial diversity. PCR primers that target conserved iron binding motifs in alkane monooxygenase (alkB) and cytochrome P450 alkane hydroxylase were used to analyze genes of alkane degrading bacteria in chronically contaminated and uncontaminated sites, using an enrichment culture with dodecane. Analysis of 16S rRNA showed that in contaminated soils BetaProteobacteria phylum populations were abu ...Более