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Sivas Yöresinde Koyun Keçi ve Kenelerde Theileria ve Babesia Türlerinin Moleküler Yöntemlerle Araştırılması

Kürşat ALTAY

This study was carried out to investigate the presence and distribution of Theileria and Babesia species with microscopic examination and reverse line blotting (RLB) techniques in sheep, goats and ticks from Sivas (Sivas, Kangal, Koyulhisar, Yıldızeli) of Central Anatolia region of Turkey. For this purpose 309 blood samples (206 sheep and 103 goats) and 478 tick samples that divided into 27 pools as the species were collected from sheep and goats. The total genomic DNAs were extracted from 309 blood samples and 27 tick pools were tested by RLB for Theileria and Babesia species. A total of 8 an ...Более

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Şanlıurfa yöresinde koyun ve keçilerde Anaplasma phagocytophilum’un moleküler yöntemlerle araştırılması

Nazir DUMANLI

This study was performed to determine the presence and prevalence of Anaplasma phagocytophilum in sheep and goats in the Şanlıurfa region. Whole blood samples were taken from a total of 208 animals (134 sheep and 74 goats) in the Şanlıurfa region. Polymerase chain reaction (PCR) using primers specific to the A. phagocytophilum 16S SSU rRNA gene of the bacterium was applied to the blood samples. The amplified DNA fragments in PCR positive samples were sequenced in order to confirm their identity. PCR amplification indicated the presence of A. phagocytophilum in 9 animals (4.33 %). A. phagocytop ...Более

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Survey of Anaplasma infections in Small Ruminants from East Part of Turkey = Doğu Anadolu Bölgesinde Koyun ve Keçilerde Anaplasma Enfeksiyonlarının Araştırılması

Kürşat ALTAY

This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma phagocytophilum in small ruminants from Bingol, Elazig, Malatya and Mus provinces. A total of 422 (291 sheep and 131 goats) blood samples were collected from apparently healthy animals. To determine of A. ovis and A. phagocytophilum in small ruminants, species-specific PCRs were set up using 60 kDA chaperonin gene (cpn60, also known as hsp60 or groEL) and 16S SSU rRNA gene primer sets, respectively. A total of 301 (71.32%) animals were found infected with A. ovis and/or A. phagocytophilum . The p ...Более

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