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Найденный: 6 Экземпляр 0.002 sn
Базы данных : Web of Science Core ... ✕Тематический рубрикатор : PCR ✕
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First Molecular Evidence of Babesia vogeli, Babesia vulpes, and Theileria ovis in Dogs from Kyrgyzstan

Ayperi Aytmırza Kızı

Tick-borne parasitic diseases cause mild to severe infections among vertebrate hosts, including dogs. Species in the genus Babesia are important tick-borne pathogens and have worldwide distributions. Although there are data on the prevalence and distribution of Babesia species among dogs around the world, there is no information available in Kyrgyzstan, according to a literature review. In this study, 337 dogs were screened by nested PCR for the presence of the 18S small subunit ribosomal RNA (18S SSU rRNA) gene of piroplasm species. Overall prevalence was 6.23 (21/337) for Babesia/Theileria s ...Более

Статья 2023 Veteriner Fakültesi Pathogens12 ( 8 ) 32 Просмотры
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Molecular Survey of Hepatozoonosis in Natural Infected Dogs: First Detection and Molecular Characterisation of Hepatozoon canis in Kyrgyzstan

Ayperi Aytmırza Kızı | Zarima CUMAKANOVA | Ayday CUNUŞOVA

Canine hepatozoonosis is a tick-borne protozoan disease and widespread in Europe, Africa, Asia and America. There is not any available data about the presence of Hepatozoon infections in dogs in Kyrgyzstan. In the study we aimed that investigate the presence of Hepatozoon canis and the prevalance of Hepatozoon infections in dogs from Kyrgyzstan using polymerase chain reaction (PCR) and sequence analysis. To determine the prevelance of hepatozoonosis in dogs, a total of 170 blood samples were applied to PCR to amplify a fragment of 666 bp found in 185 SSU rRNA gene of Hepatozoon spp. The PCR re ...Более

Статья 2019 Veteriner Fakültesi Kafkas Üniversitesi Veteriner Fakültesi Dergisi25 ( 1 ) 60 Просмотры
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First molecular survey of piroplasm species in cattle from Kyrgyzstan

Ruslan SALIKOV

Bovine piroplasmosis is a tick-borne disease caused by apicomplexan hemoparasites of the genera Theileria and Babesia. This study was carried out to assess the presence and frequency of piroplasm parasites in apparently healthy cattle in Kyrgyzstan. A total of 454 blood samples were collected from animals of various ages in eight villages located in the Chu valley and around the Lake Issyk Kul. The hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene was amplified with a set of primers specific targeting members of the genera Theileria and Babesia. Amplified PCR products were hybridize ...Более

Статья 2019 Veteriner Fakültesi Parasitology Research118 ( 8 ) 49 Просмотры
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CHARACTERIZATION OF TEA (CAMELLIA SINENSISL.) GENOTYPES GROWN IN TURKEY BY ISSR MARKERS

Ahmet AYGÜN

The aim of this study was to determine the genetic diversity of 18 tea (Camellia sinensis) genotypes and 6 varieties collected from different provinces in the Black Sea region of Turkey. DNA isolation was performed according to the CTAB method of tea samples. PCR was performed for all DNA samples with 59 ISSR primers. In order to determine the genetic relationship between tea genotypes among these primers, 15 polymorphic ISSR primers were used. At the end of the PCR procedures, the polymorphism rate was determined as 77?rom the bands formed. As a result of the analysis of products obtained by ...Более

Статья 2021 Ziraat Fakültesi Applied Ecology and Environmental Research19 ( 5 ) 96 Просмотры
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First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan = Kırgızistan’da Asemptomatik Barınak Köpeklerinde Mycoplasma haemocanis ve Candidatus Mycoplasma haematoparvum İçin İlk Moleküler Kanıt

Ayperi Aytmırza Kızı | Zarima CUMAKANOVA | Ayday CUNUŞOVA

Mycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp) have been investigated using species specific PCR and sequencing in 170 dogs from Kyrgyzstan. Maximum likelihood estimation (MLE) of the infection rates with 95% confidence intervals (CI) was calculated. The molecular prevalence of hemoplasma infection was 5.29% (CI 2.57-9.34). It was found that, five (2.94%, CI 1.06-6.22) samples were found to be infected with Mhc, one (0.59%, CI 0.03-2.57) sample with CMhp and three (1.76%, CI 0.44-4.52) samples with both species. These results demonstrate that dogs can be exposed to ...Более

Статья 2020 Veteriner Fakültesi Kafkas Üniversitesi Veteriner Fakültesi Dergisi26 ( 1 ) 34 Просмотры
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First Molecular Detection of Dirofilaria immitis and D. repens in Dogs from Kyrgyzstan

Ayperi Aytmırza Kızı

Background Dirofilaria immitisandDirofilaria repensare the causative agents of cardiopulmonary and subcutaneous dirofilariosis, respectively. This neglected disease mainly seen in dogs, cats and wild carnivores is re-emerging recent years. No study was conducted on dirofilariosis in dogs in Kyrgyzstan. Purpose The goal of this study was to investigateDirofilariaspecies using PCR and sequencing in dogs from Kyrgyzstan. Method Dirofilariaspp. infection in dogs was screened via convential PCR and sequencing in 337 dogs from Kyrgyzstan. Result The overall prevalence ofDirofilariaspp. was 0.59% (2/ ...Более

Статья 2020 Veteriner Fakültesi Acta Parasitologica65 ( 4 ) 59 Просмотры
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