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Cotton Biotechnology: An Efficient Gene Transfer Protocol via Agrobacterium tumefaciens for a Greater Transgenic Recovery

İbrahim İlker ÖZYİĞİT | Yılmaz KAYA

Due to its economic worth, cotton (Gossypium hirsutum L.) is grown in almost 70 countries and provides income for more than 250 million people. Therefore, producing cotton with having some desired characteristics that includes extended biotic and abiotic stress tolerance, improved fiber quality, promoted nutritional content and increased yield is the main objective for cotton biotechnology. To achieve this goal, many tissue culture and gene transfer techniques are being developed and used throughout the years. As applications for the gene transfer, the Agrobacterium-mediated, particle bombardm ...Более

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Cotton Agriculture in Turkey and Worldwide Economic Impacts of Turkish Cotton

İbrahim İlker ÖZYİĞİT

Cotton is a product that provides wealth for humanity with its widespread and compulsory usage, and alongside creating employment opportunities in the producer countries. Due to the availability of suitable land for agriculture, seven countries including Turkey produce close to 80% of cotton worldwide. The average cotton yield in Turkey is above the world average, placing the country close to first place among the major producing countries. On the other hand, Turkey is one of the main countries engaged in organic cotton production by using only non-transgenic seeds and therefore, its cotton as ...Более

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An investigation on environmental pollution due to essential heavy metals: a prediction model through multilayer perceptrons

İbrahim İlker ÖZYİĞİT

This research is to predict heavy metal levels in plants, particularly in Robinia pseudoacacia L., and soils using an effective artificial intelligence approach with some ecological parameters, thereby significantly eliminating common defects such as high cost and seriously tedious and time-consuming laboratory procedures. In this respect, the artificial neural network (ANN) is employed to estimate the concentrations of essential heavy metals such as Fe, Mn and Ni, depending on the Cu and Zn concentrations of plant and soil samples collected from five different locations. The derived relative ...Более

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Comparative analyses of phytochelatin synthase (PCS) genes in higher plants

İbrahim İlker ÖZYİĞİT

Plants employ various defence strategies to ameliorate the effects of heavy metal exposures, leading to re-establishment of metal homeostasis. One of the strategies includes the biosynthesis of main heavy metal detoxifying peptides phytochelatins (PCs) by phytochelatin synthase (PCS). In the present study, 14 PCS homologues were identified in the genomes of 10 selected plants. The size of these PCSs was 452-545 amino acid residues, with characteristic phytochelatin and phytochelatin_C domains. The N-terminal site of the proteins is highly conserved, whereas the C-terminal site is less conserve ...Более

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Abiotic stress-induced regulation of antioxidant genes in different Arabidopsis ecotypes: microarray data evaluation

İbrahim İlker ÖZYİĞİT

Although stresses induce generation of reactive oxygen species (ROS), which are highly reactive and toxic, and cause severe damage to cellular components; plants have very efficient enzymatic ROS-scavenging mechanisms. Despite the substantial knowledge produced about these enzymes, we still have limited knowledge regarding their expression patterns in relation to the stress type, duration and strength. Thus, taking advantage of microarray data, this work evaluated the abiotic stresses (salt, cold, heat and light) induced regulation of six antioxidant enzymes, superoxide dismutase (SOD), catala ...Более

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Economic Impacts of Bt (Bacillus thuringiensis) Cotton

İbrahim İlker ÖZYİĞİT

In recent years, high-yield production has gained importance due to the decrease in agricultural fields in the world and the rapid increase in the population. Cotton is an important source of income for farmers worldwide and is an important crop, especially for the textile industry. Bt-cotton technology, which entered our lives in 1996, has been quickly adopted in many developed and developing countries. It has been shown to provide advantages in many areas. In particular, the economic growth is gaining importance every year. Additionally, it has also brought an environmentally friendly soluti ...Более

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Lemna minor, a hyperaccumulator shows elevated levels of Cd accumulation and genomic template stability in binary application of Cd and Ni: a physiological and genetic approach

İbrahim İlker ÖZYİĞİT

In this study, to determine whether having potential to be used as hyperaccumulator for Cd and Ni, numerous experiments were designed for conducting assessments for physiological and genotoxic changes along with defining possible alterations on mineral nutrient status of Lemna minor L. by applying Cd-Ni binary treatments (0, 100, 200 and 400 mu M). Our study revealed that there were increases in the concentrations of B, Cr, Fe, K, Mg, and Mn whereas decreases were noticed in the concentrations of Na and Zn and the levels of Ca were inversely proportional to Cd-Ni applications showing tendency ...Более

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Assessment of pollution at the former uranium waste dumpsite near kaji-Say Village/Kyrgyzstan: a genetic and physiological investigation

İbrahim İlker ÖZYİĞİT | Bermet KIDIRALİYEVA | Kadırbay ÇEKİROV | Gülbübü KURMANBEKOVA | Nurzat ŞAYKİYEVA

Kyrgyzstan still suffers from the past practices of radioactive industry operated by the Soviet Union that caused significant impact on ecosystems in this region, especially related with storage of uranium in inadequate conditions. There are at least 50 abandoned sites used as radioactive waste dumps in the country. Due to the pressure of natural and anthropogenic reasons, the structural foundations of mine waste dumps are gradually losing their integrities. Here, particular interest of our research is to reveal current state of radioactive contamination and to make evaluation on the radiologi ...Более

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Morphological variation, genetic diversity and phylogenetic relationships of Hypericum triquetrifolium Turra populations from Tunisia

İbrahim İlker ÖZYİĞİT

Hypericum triquetrifolium Turra is an ecologically, medicinally and economically important species in Tunisia. Thirty-six Hypericum individuals sampled from 6 northern Tunisian locations were investigated for their diversity and relationships using 10 inter-simple sequence repeats (ISSR) markers and 10 morphological features at vegetative stage. The phylogenetic analysis, using 308 bp of sequenced ITS1 region, identified the Hypericum individuals as H. triquetrifolium that clustered with members of genus Hypericum section 9, 9a, 9b and 27, in agreement with the previous molecular classificatio ...Более

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Deciding Heavy Metal Levels in Soil Based on Various Ecological Information through Artificial Intelligence Modeling

İbrahim İlker ÖZYİĞİT

The aim of this paper is to decide on heavy metal levels based on ecological parameters by effectively eliminating common disadvantages such as high cost and serious time-consuming laboratory procedures via an effective artificial intelligence approach. Therefore, this study is hinged on an artificial intelligence technique, ANN, because of its low cost and high accuracy in overcoming the mentioned limitations and obstacles in the determination process of the amounts of elements. The ANNs have thus been employed to determine essential heavy metals, such as Fe, Mn, and Zn depending on Ca, K, an ...Более

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Exploration of two major boron transport genes BOR1 and NIP5;1 in the genomes of different plants

İbrahim İlker ÖZYİĞİT

Boron (B) is an essential plant micronutrient but studies regarding its transport are still limited to a few plants. This work identified two major B transport sequences in plants, NIP5;1 boric acid channel protein and BOR1 transporter. 80 BOR1 and 34 NIP5;1 homologs were identified in 18 different plant genomes. BOR1 homologs had a HCO3-transporter domain, 649-737 amino-acid residues with mainly basic nature, putative 8-11 transmembrane domains (TMDs) and 11-13 exons. NIP5;1 homologs had a MIP family domain, 294-311 amino-acid residues with basic nature, 5-6 putative TMDs and 3-5 exons. Tyros ...Более

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Genetic Diversity and Phylogenetic Analyses of Turkish Cotton (Gossypium HirsutumL.) Lines Using ISSR Markers and ChloroplasttrnL-FRegions

İbrahim İlker ÖZYİĞİT

In this study, genetic diversity and phylogenetic relationships of 22 cotton (Gossypium hirsutumL.) lines widely cultivated in Turkey were investigated. For this purpose, 20 ISSR primers were used, and five of which generated amplification products. The polymorphic locus ratio was calculated as 87.21% and a moderate level of genetic diversity was found between the genotypes. Percentage of the highest polymorphic locus was calculated as 12.79% for the Beren line, whereas ADN 98, ADN 710, and ozbek 105 lines showed the lowest value with 0.00%.trnL-Fintergenic spacer sequences were used in order ...Более

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