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In this study, the presence, prevalence, and genotypes of Anaplasma phagocytophilum, A. ovis, and A. capra in sheep were investigated based on 16 S SSU rRNA, groEL, and gtlA gene-specific polymerase chain reaction (PCR), respectively. The sequences of the genes were used for detection of the phylogenetic position of the species. Additionally, a restriction fragment length polymorphism (RFLP) were carried out for discrimination of A. phagocytophilum and related variants (A. phagocytophilum-like 1 and 2). The prevalence of Anaplasma spp. was found as 25.8 (101/391), while it was found that A. ov ...Более
Hemoplasma species cause clinical infections ranging from asymptomatic to life-threatening in cattle. To date, two hemoplasma species, Mycoplasma wenyonii (M. wenyonii) and Candidatus Mycoplasma haemobos (Ca. M. haemobos), have been identified from cattle. There is no data on the presence and distributions of bovine hemoplasma species in cattle in Kyrgyzstan, notwithstanding their potential negative economic impact on cattle production industries. In the current study, we investigated the molecular presence and distributions of bovine hemoplasma species from 358 cattle samples obtained from di ...Более
Background Dirofilaria immitisandDirofilaria repensare the causative agents of cardiopulmonary and subcutaneous dirofilariosis, respectively. This neglected disease mainly seen in dogs, cats and wild carnivores is re-emerging recent years. No study was conducted on dirofilariosis in dogs in Kyrgyzstan. Purpose The goal of this study was to investigateDirofilariaspecies using PCR and sequencing in dogs from Kyrgyzstan. Method Dirofilariaspp. infection in dogs was screened via convential PCR and sequencing in 337 dogs from Kyrgyzstan. Result The overall prevalence ofDirofilariaspp. was 0.59% (2/ ...Более