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Устойчивое развитие ООН : Ликвидация голода ... ✕Ученые : Saykal BOBUŞOVA ✕

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Устойчивое развитие ООН [3]

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Dr. Saykal BOBUŞOVAZiraat Fakültesi

Ликвидация голодаПоложить конец голоду, достичь продовольственной безопасности и здорового питания, и поддерживать устойчивое сельское хозяйство

Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy

Saykal BOBUŞOVA | Tinatin DOOLOTKELDİEVA

Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS se ...Более

Статья 2023 Ziraat Fakültesi Journal of Fungi9 ( 10 ) 26 Просмотры

Identification of Microbial Populations Present in Agricultural and Nonarable Soils in the Talas Valley, Northern Kyrgyzstan, in Autumn

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Mahabat KONURBAYEVA

Soil bacterial and fungal communities were investigated in relation to soil type and farm management practices after vegetation harvesting in autumn. Soils from fields cultivated with Phaseolus vulgaris (bean) and Pyrus comminus (pear) and nonarable, natural areas were studied. Microbial diversity was analysed using cultivation-dependent methods (isolation of pure cultures) and cultivation-independent methods (direct extraction of DNA from soil, followed by PCR amplification of the 16S rRNA and 18S rRNA genes). The use of cultivation-dependent methods revealed that there were no differences in ...Более

Статья 2023 Ziraat Fakültesi Applied and Environmental Soil Science2023 17 Просмотры

Microbial communities of vegetable seeds and biocontrol microbes for seed treatment

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Thirty-one species of vegetable seeds from Kyrgyzstan's State Register were analysed for mycological and bacterial composition using various methods, from simple visual to molecular. The modified roll-towel method simultaneously assesses the degree of contamination and seed germination. Screening in agar medium allows analysis of species on the outer and inner surfaces of vegetable seeds that are not detected using the roll-towel method. As a result of the phytopathological examination, 15 fungal and three bacterial species were found. Direct DNA extraction and amplification of the universal 1 ...Более

Статья 2022 Ziraat Fakültesi Seed Science and Technology50 ( 1-3 ) 34 Просмотры

Characterization of Beauveria bassiana isolates from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Sezim COLDOŞBEKOVA

We report on the enzootic foci of the insect pathogenic fungus, Beauveria bassiana, found in high meadows in the middle mountain steppes of Kyrgyzstan, at elevations from 1000 m to 2200 m. The growth characteristics of various B. bassiana isolates on different media and as a function of temperature were studied. In addition, the ability of the fungal isolates to produce enzymes with amylase, protease and lipase activities was investigated. Dense biomass production on inexpensive solid media (oatmeal and bean oil meal) produced conidia used for insect bioassays targeting white grub larvae (Phyl ...Более

Статья 2019 Ziraat Fakültesi Journal of Invertebrate Pathology167 36 Просмотры

Isolation, Identification, and Characterization of the Nematophagous Fungus Arthrobotrys oligospora from Kyrgyzstan

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Ayperi MURATBEKOVA

Purpose Predatory fungi have been the subject of fundamental studies and their potential as biological control agents against parasitic plant nematodes has been assessed. The aim of the present study was to isolate and identify predatory fungi, performing in vitro and in vivo screening to select highly active strains to control parasitic nematodes. Methods Different nutrient media were used to isolate predatory fungi and determine their morphological and cultural properties. Identification was performed by classical and molecular biology methods. In vitro and in vivo screening was conducted to ...Более

Статья 2021 Ziraat Fakültesi Acta Parasitologica66 ( 4 ) 95 Просмотры

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